Laboratory Safety ManualLaboratory Safety Manual
Purpose of This Manual
The purpose of this manual is to supplement the basic regulatory requirements of the OSHA Laboratory Standard for the development of a Chemical Hygiene Plan and to provide laboratories with useful recommendations that can help achieve compliance with the intent of the OSHA Lab Standard. Throughout this document, areas where regulatory or University requirements exist will be clearly identified using words such as “must”, “required”, “shall”, and “it is the responsibility”, etc. All other information provided within this document are recommendations that Environment, Health, and Safety (EHS) encourages laboratories to follow as best management practices.
To take advantage of the Internet, this document is formatted to be a “front door” to other resources, including useful web links. Where appropriate, web links will be embedded within the document and identified as a hyperlinked word that can be clicked on to view the webpage. Please note, by clicking on these external resources you will be leaving the Laboratory Safety Manual and will need to click the “Back” button on your browser to return to the manual. For those internal hyperlinks, including the table of contents, you can move around the document by clicking on the back and forward hyperlink arrow buttons located in the upper left hand side of your toolbar. If this toolbar is not visible, then go to your menu command “View”, then “Toolbars”, and then select the “Web” or “Navigation” button to make the toolbar and hyperlink arrow buttons accessible. You can search both the PDF and web-based versions of the Laboratory Safety Manual using the function CTRL-F.
If you encounter a broken web link, please send an email to askEHS@cornell.edu and include the section and page number of the manual and the name of the link you were trying to reach. If you have any suggestions to improve this document, including sharing useful web links or would like to request a section or topic to be added, then please send an email to the Chemical Hygiene Officer. This Laboratory Safety Manual should be considered a living document and will be reviewed at least annually and updated with your participation, comments, and suggestions.
TABLE OF CONTENTSTABLE OF CONTENTS
Chapter 1.0 - Introduction
Chapter 1 - IntroductionChapter 1 - Introduction
The Cornell University Health and Safety Policy 8.6 outlines safety responsibilities and training requirements to ensure individual and institutional compliance with relevant environmental health and safety laws, regulations, policies, and guidelines. This Laboratory Safety Manual supplements the University’s Chemical Hygiene Plan and recommendations for good laboratory practices to serve as a useful resource and to assist laboratories in designing their own site-specific laboratory safety procedures to meet these requirements.
The Occupational Safety and Health Administration (OSHA) regulation 29 CFR 1910.1450, "Occupational Exposure to Hazardous Chemicals in Laboratories”, mandates health and safety practices and procedures in laboratories that use hazardous chemicals. The Standard became effective May 1, 1990 and requires that a Chemical Hygiene Plan be developed for each laboratory workplace. The purpose of the Laboratory Standard is to protect laboratory employees from harm due to chemicals while they are working in a laboratory. This regulation applies to all employers engaged in the laboratory use of hazardous chemicals which OSHA defines as:
"Laboratory" means a facility where the "laboratory use of hazardous chemicals" occurs. It is a workplace where relatively small quantities of hazardous chemicals are used on a non-production basis.
"Laboratory scale" means work with substances in which the containers used for reactions, transfers, and other handling of substances are designed to be easily and safely manipulated by one person. "Laboratory scale" excludes those workplaces whose function is to produce commercial quantities of materials.
“Hazardous chemical” means a chemical for which there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed employees. The term “health hazard” includes chemicals which are carcinogens, toxic or highly toxic agents, reproductive toxins, irritants, corrosives, sensitizers, hepatotoxins, nephrotoxins, neurotoxins, agents which act on the hematopoietic systems and agents which damage the lungs, skins, eyes, or mucous membranes. Appendix A and Appendix B of the Hazard Communication standard (29 CFR 1910.1200) provide further guidance in defining the scope of health hazards and determining whether or not a chemical is to be considered hazardous for the purposes of this standard.
A complete description of definitions applicable to laboratories can be found in the OSHA Laboratory Standard.
In all other areas that use chemicals, but do not fall under the OSHA definition of a “laboratory”, the OSHA regulation 29 CFR 1910.1200 – "Hazard Communication Standard” applies.
Most laboratories at Cornell using chemicals are subject to the requirements of the Laboratory Standard. In addition to employees who ordinarily work full-time within a laboratory space, other employees (such as office, custodial, maintenance and repair personnel) who regularly spend a significant amount of their time within a laboratory environment as part of their duties, also may fall under the requirements of the Laboratory Standard. OSHA considers graduate students who get paid for working in a lab as employees who are subject to the requirements of the Laboratory Standard.
The OSHA Laboratory Standard requires employers to develop a Chemical Hygiene Plan (CHP), designate a Chemical Hygiene Officer, and ensure laboratory employees are provided with the proper information and training, including knowing the location of the Chemical Hygiene Plan, and how to work safely in their labs. The main goals of the OSHA Laboratory Standard and the requirement to develop a Chemical Hygiene Plan are; to protect employees from health hazards associated with use of hazardous chemicals in the laboratory, and keep exposures below the permissible exposure limits as specified in 29 CFR Part 1910, subpart Z – Toxic and Hazardous Substances and other resources such as NIOSH and ACGIH. In addition to other requirements, the OSHA Lab Standard specifies the Chemical Hygiene Plan to include “criteria the employer will use to determine and implement control measures to reduce employee exposure to hazardous chemicals including engineering controls, the use of personal protective equipment and hygiene practices; particular attention shall be given to the selection of control measures for chemicals that are known to be extremely hazardous.”
Cornell EHS has taken responsibility for maintaining an institutional Chemical Hygiene Plan. Each college, center, department, or laboratory may adopt or modify this plan or write their own chemical hygiene plan as long as the requirements of the OSHA Laboratory Standard are met. It is assumed if a college, center, department, or laboratory has not developed their own chemical hygiene plan, then that unit or laboratory has adopted the Cornell University Chemical Hygiene Plan. The Cornell University CHP is maintained by the Department of Environmental Health and Safety (EHS). The campus CHP is designed to supplement department and laboratory specific safety manuals and procedures that already address chemical safety in laboratories.
- OSHA Lab Standard
- OSHA Lab Standard Appendix A
- Environment, Health and Safety Policy 8.6
- University Policy 8.3 - Emergency Planning
- OSHA Hazcom Standard
- OSHA Lab Standard Appendix B
- OSHA Toxic and Hazardous Substance Standard
- Department Safety Representative (DSR) Program
1.1 Chemical Hygiene Plan Accessibility1.1 Chemical Hygiene Plan Accessibility
The OSHA Laboratory Standard requires the Chemical Hygiene Plan (CHP) to be readily available to employees, employee representatives and, upon request, to the Assistant Secretary of Labor for Occupational Safety and Health, U.S. Department of Labor, or designee. This means laboratory employees working with hazardous chemicals in a laboratory must know the location of the CHP, be familiar with the contents, and be able to produce the CHP for any state or federal regulatory inspectors upon request. While EHS recommends a hard copy be kept in the laboratory, electronic access is acceptable and encouraged.
It is the responsibility of Principal Investigators and laboratory supervisors to ensure that personnel working in laboratories under their control are familiar with the contents and location of the Chemical Hygiene Plan, including any lab specific standard operating procedures and any department or college level laboratory safety manuals, policies, and procedures.
1.2 Laboratory Safety Responsibilities1.2 Laboratory Safety Responsibilities
The ultimate responsibility for health and safety within laboratories lies with each individual who works in the laboratory; however, it is the responsibility of the Principal Investigator, Faculty, and laboratory supervisor to ensure that employees (including visiting scientists, fellows, volunteers, temporary employees, and student employees) have received all appropriate training, and have been provided with all the necessary information to work safely in laboratories under their control. Principal investigators, Faculty, and Lab Supervisors have numerous resources at their disposal for helping to ensure a safe and healthy laboratory that is compliant with state and federal regulations. A listing of EHS staff, responsibilities, and services available to campus personnel can be found on the EHS Subject Matter Experts.
1.2.1 Environment, Health and Safety1.2.1 Environment, Health and Safety
Environment, Health and Safety (EHS) will provide technical information and program support to assist in compliance with the OSHA Laboratory Standard. This includes developing policies, recommendations and guidelines (as found in this Laboratory Safety Manual), developing and providing training programs designed to meet regulatory requirements, and serving as consultants in providing health and safety information to laboratory personnel. EHS will maintain the campus Chemical Hygiene Plan and the institutional Chemical Hygiene Officer responsibilities.
1.2.2 Chemical Hygiene Officer1.2.2 Chemical Hygiene Officer
The role of the Chemical Hygiene Officer (CHO) is to facilitate the implementation of the campus Chemical Hygiene Plan and this Laboratory Safety Manual in laboratories across campus and outlying facilities, and to serve as a technical resource to the campus laboratory community. The Associate CHO will act in the absence of the CHO. The names of the current CHO and the Associate CHO can be found in EHS Subject Matter Experts. The major duties of the Chemical Hygiene Officer are:
- Work with campus stakeholders to evaluate, implement, review annually, and make updates as needed to the Chemical Hygiene Plan and Laboratory Safety Manual.
- Provide technical expertise to the laboratory community in the area of laboratory safety and health, and serve as a point of contact to direct inquiries to other appropriate resources.
- Ensure that guidelines are in place and communicated for particularly hazardous substances regarding proper labeling, handling, use, and storage, selection of proper personal protective equipment, and facilitating the development of standard operating procedures for laboratories using these substances.
- Serve as a resource to review academic research protocols and standard operating procedures developed by Principal Investigators and department personnel for the use, disposal, spill cleanup, and decontamination of hazardous chemicals, and the proper selection and use of personal protective equipment.
- Coordinate the acquisition, testing and maintenance of fume hoods and emergency safety showers and eyewashes in all laboratories where hazardous chemicals are used.
- Conduct laboratory safety training sessions for laboratory personnel and upon request, assist laboratory supervisors in developing and conducting hands-on training sessions with employees.
- Review reports for laboratory incidents, accidents, chemical spills, and near misses and recommend follow up actions where appropriate.
- Stay informed of plans for renovations or new laboratory construction projects and serve as a resource in providing code citations and internal standards to assist with the design and construction process.
- Keep the senior administration informed on the progress of continued implementation of the Chemical Hygiene Plan and Laboratory Safety Manual and bring campus-wide issues affecting laboratory safety to their attention.
1.2.3 Deans, Directors, and Department Chairpersons1.2.3 Deans, Directors, and Department Chairpersons
The Deans, Directors, and Department Chairpersons are responsible for laboratory safety within their department(s) and must know and understand the Environment, Health and Safety Policy 8.6 and know and understand the guidelines and requirements of the Laboratory Safety Manual. In addition to the responsibilities outlined within the University Health & Safety Policy, the laboratory safety responsibilities of Deans, Directors, and Department Chairpersons - which can be delegated to other authorized personnel within the department such as a Department Safety Representative (DSR) - are:
- Be familiar with and implement the University Health & Safety Policy within units under their control or designate a person in the department (such as the DSR) with the authority to carry out these requirements.
- Communicate and implement the University Health and Safety Policy and its requirements to faculty, staff (including temporary employees), visiting scholars, volunteers, and students working in laboratories within their units.
- Assist the Chemical Hygiene Officer with implementation of the Chemical Hygiene Plan and Laboratory Safety Manual.
- Ensure laboratory personnel develop and adhere to proper health and safety protocols.
- Direct individuals under their supervision, including but not limited to - Principal Investigators, supervisors, regular and temporary employees, visiting professors, and students employees - to obtain any required safety and health training before working with hazardous chemicals, biohazardous agents, radiation, and/or other physical/mechanical hazards found within their working or learning environments.
- Determine and ensure that safety needs and equipment for units/departments are met (e.g., engineering controls, training, protective equipment) and ensure corrective measures for noncompliance items identified in safety audits are corrected promptly.
- Encourage the formation of a college and/or department safety committee(s).
- Keep the DSR, Building Coordinator, and Chemical Hygiene Officer informed of plans for renovations or new laboratory construction projects.
- Ensure college and departmental procedures are established and communicated to identify and respond to potential accidents and emergency situations.
- Notify EHS before a faculty member retires or leaves the University so proper laboratory decommissioning occurs. For more information, see the Lab Decommissioning Process and Checklist in Appendix E.
- Establish college and departmental priorities, objectives, and targets for laboratory safety and health performance. Obtain assistance and guidance from EHS when necessary.
- Ensure college and departmental laboratory participation in EHS Research Area Inspections as a means to regularly check performance against regulatory requirements and identify opportunities for improvement.
- Ensure that research areas within their departments and units are registered using HASP in a timely manner upon notification by EHS and updated annually.
1.2.4 Principal Investigators, Faculty, and Laboratory Supervisors1.2.4 Principal Investigators, Faculty, and Laboratory Supervisors
Principal Investigators, faculty, and laboratory supervisors are responsible for laboratory safety in their research or teaching laboratories. In addition to the responsibilities outlined within the Environment, Health and Safety Policy 8.6, the laboratory safety duties of Principal Investigators, faculty, and laboratory supervisors (which can also be delegated to other authorized personnel within the laboratory) are:
- Implement and communicate the Environment, Health and Safety Policy 8.6 and all other University safety practices and programs, including the guidelines and procedures found within the Laboratory Safety Manual, in laboratories under your supervision or control.
- Establish laboratory priorities, objectives and targets for laboratory safety, health and environmental performance.
- Communicate roles and responsibilities of individuals within the laboratory relative to environmental, health, and safety according to this Laboratory Safety Manual.
- Conduct hazard evaluations for procedures conducted in the laboratory and maintain a file of standard operating procedures documenting those hazards.
- Ensure that specific operating procedures for handling and disposing of hazardous substances used in their laboratories are written, communicated, and followed and ensure laboratory personnel have been trained in these operating procedures and use proper control measures.
- Attend required health and safety training.
- Require all staff members and students under their direction to obtain and maintain required health and safety training commensurate with their duties and/or department requirements.
- Participate in EHS Research Area Inspections with their laboratory employees or designate someone in the laboratory to conduct these inspections.
- Ensure that all items identified during annual EHS research area inspections are corrected in a timely manner.
- Ensure that all appropriate engineering controls including chemical fume hoods and safety equipment are available and in good working order in their laboratories. This includes notifying EHS when significant changes in chemical use may require a re-evaluation of the laboratory ventilation.
- Ensure procedures are established and communicated to identify the potential for, and the appropriate response to accidents and emergency situations.
- Ensure that all incidents and near misses occurring in their laboratories are reported to their Director or Department Chairperson and/or Department Safety Representative and that a written Injury/Illness Report is filed with EHS for each injured person.
- Ensure laboratory personnel under your supervision know and follow the guidelines and requirements contained within the Laboratory Safety Manual.
- Follow the guidelines identified within this manual as Principal Investigator and laboratory supervisor responsibilities. A compiled version of these responsibilities can be found in the Appendix C.
- Notify EHS before retiring or leaves the University and ensure proper laboratory decommissioning occurs. For more information, see the Lab Decommissioning Process and Checklist in Appendix E.
- Keep EHS, the Department Safety Representative, Department Chairperson informed of plans for renovations or new laboratory construction projects.
- Ensure that research areas under their supervision are registered using HASP in a timely manner upon notification by EHS and updated annually.
1.2.5 Laboratory Employees1.2.5 Laboratory Employees
Laboratory employees are those personnel who conduct their work in a laboratory and are at risk of possible exposure to hazardous chemicals on a regular or periodic basis. These personnel include laboratory technicians, instructors, researchers, visiting researchers, administrative assistants, graduate assistants, student aides, student employees, and part time and temporary employees.
In addition to the responsibilities outlined within the Environment, Health and Safety Policy 8.6, the laboratory safety duties of laboratory employees are:
- Comply with the Environment, Health and Safety Policy 8.6 and all other health and safety practices and programs by maintaining class, work, and laboratory areas safe and free from hazards.
- Know the location of the Chemical Hygiene Plan and how to access safety data sheets (SDS).
- Attend health and safety training as designated by your supervisor.
- Inform your supervisor or instructor of any safety hazards in the workplace, classroom, or laboratory, including reporting any unsafe working conditions, faulty fume hoods, or other emergency safety equipment to the laboratory supervisor.
- Ensure an SDS is present for all new chemicals you purchase (either sent with the original shipment or available online. Review the SDSs for chemicals you are working with and check with your laboratory supervisor or principal investigator if you ever have any questions.
- Conduct hazard evaluations with your supervisor for procedures conducted in the laboratory and maintain a file of standard operating procedures documenting those hazards.
- Be familiar with what to do in the event of an emergency situation.
- Participate in laboratory self inspections and annual EHS Research Area Inspections.
- Follow the standard operating procedures for your laboratory and incorporate the guidelines and requirements outlined in this Laboratory Safety Manual into everyday practice.
1.2.6 Building Coordinators1.2.6 Building Coordinators
Building Coordinators serve as an important conduit for information with regard to building wide issues. This information includes reporting and coordinating routine maintenance issues, scheduling building shutdowns, and communicating building wide maintenance and repairs and building system shutdowns to all occupants.
Laboratory safety responsibilities of Building Coordinators include:
- Comply with the Environment, Health and Safety policy 8.6 and all other University health and safety practices and programs by maintaining common building areas safe and free from hazards.
- Attend health and safety training as designated by your supervisor.
- Keep the DSR, Department Chairperson, and EHS informed of plans for renovations or new laboratory construction projects, and the laboratory needs of new faculty and staff.
- Notify EHS of faculty retiring or leaving the University so appropriate laboratory decommissioning occurs. For more information, see the Lab Decommissioning Process and Checklist in Appendix E.
- Ensure that ticket requests for safety equipment (such as fume hoods and emergency eyewash/showers) and other laboratory equipment are processed in a timely manner.
- Ensure that requests from EHS related to building-wide laboratory safety issues are addressed.
- Be aware of building issues that could impact the health and safety of laboratory personnel and contact EHS at 607-255-8200 whenever building-wide health and safety issues occurs in laboratories.
- Be familiar with what to do in the event of an emergency situation.
- Assist emergency responders during emergencies by serving as a resource for control of building control systems (ventilation, turning off water main, etc.).
1.2.7 Department Safety Representatives1.2.7 Department Safety Representatives
The Department Safety Representative (DSR) serves a very important function in implementing the Chemical Hygiene Plan and Laboratory Safety Manual within the department. The role of the DSR is to assist the director, unit head, and/or department chairperson meet their responsibilities for safety and compliance as described in the Environment, Health and Safety Policy 8.6. A detailed description of DSR roles and responsibilities can be found in the separate document – Department Safety Representative Program.
Laboratory safety responsibilities of DSRs include:
- Comply with the Environment, Health and Safety policy 8.6 and all other University health and safety practices and programs.
- Request and coordinate assistance from EHS and other organizations that can provide guidance, training, and other services to assist laboratory personnel.
- Assist directors, unit heads, department chairpersons, supervisors, and individuals within the areas they represent to establish departmental, unit, or facility-wide safety programs, priorities, objectives and targets for safety, health, and environmental performance.
- Assist directors, unit heads, department chairpersons, supervisors, and individuals to identify (with assistance and guidance from EHS) if the safety needs for the areas they represent are met (e.g., training, protective equipment, acquisition of safety equipment, and corrective measures including noncompliance items identified in safety inspections).
- Encourage the formation of, and participate on college, unit, departmental, and/or facility-wide safety committee(s).
- Collaborate with unit Emergency Coordinator(s) on emergency planning efforts, response, and implementation of University Policy 8.3 - Emergency Planning.
- Work with EHS to stay knowledgeable about safety, health, and environmental services available, the University health and safety policies and procedures that apply to, and the health and safety issues that occur within the areas they represent.
- Communicate to individuals working within the areas they represent about health and safety policies and procedures, including this Laboratory Safety Manual, and the safety, health, and environmental services available to them.
- Conduct and/or facilitate routine inspections of work areas in the areas they represent using tools and resources provided by EHS, including participation in EHS Research Area Inspections. Facilitate corrective actions for any issues identified with the support and participation of EHS, including bringing issues of noncompliance to the attention of directors, unit heads and department chairpersons.
- Promote safety, health, and environmental training program and workshops (particularly EHS trainings) throughout the areas they represent by distributing fliers and EHS newsletters, and forwarding EHS training announcements and other announcements via email or hardcopy. Inform individuals working in areas they represent about the requirements to obtain necessary training as identified by their supervisor, department, college and EHS.
- Serve as a “conduit for information exchange” through facilitation and dissemination of safety, health and environmental information (particularly information sent out by EHS) to all personnel, including visiting faculty and researchers, and student employees, within the areas they represent.
- Communicate with supervisors in the areas they represent that all incidents and near misses should be reported and that a written Injury/Illness Report is completed.
- Attend EHS training programs (and other safety, health, and environmental training programs and workshops) to increase and maintain knowledge about safety, health, and environmental issues that are applicable to the areas they represent.
- Attend University DSR meetings and other college or unit level safety, health, and environmental related meetings and serve as the liaison for the areas they represent at these meetings.
- Be aware that changes in chemical use in a particular laboratory may require a re-evaluation of the laboratory ventilation.
- Notify EHS before a faculty member retires or leaves the University or laboratory groups move so proper laboratory decommissioning can occur. For more information, see the Lab Decommissioning Process and Checklist in Appendix E.
Chapter 2 - Engineering ControlsChapter 2 - Engineering Controls
Engineering controls are considered the first line of defense in the laboratory for the reduction or elimination of the potential exposure to hazardous chemicals. Examples of engineering controls used in laboratories at Cornell include dilution ventilation, local exhaust ventilation, chemical fume hoods, glove boxes and other containment enclosures, as well as ventilated storage cabinets.
The OSHA Laboratory Standard requires that "fume hoods and other protective equipment function properly and that specific measures are taken to ensure proper and adequate performance of such equipment." General laboratory room ventilation is not adequate to provide proper protection against bench top use of hazardous chemicals. Laboratory personnel need to consider available engineering controls to protect themselves against chemical exposures before beginning any new experiment(s) involving the use of hazardous chemicals.
The proper functioning and maintenance of fume hoods and other protective equipment used in the laboratory is the responsibility of a variety of service groups. Facilities Services and Engineering, Building Coordinators, EHS, and other groups service equipment such as fire extinguishers, emergency eyewash and showers, and mechanical ventilation. Periodic inspections and maintenance by these groups ensure proper functioning and adequate performance of these important pieces of protective equipment.
- American Glove Box Society
- OSHA Lab Standard
- EHS Online Training Programs
- Safe Fume Hood Use Posting
- Fume Hood Do Not Use Sign (docx)
- Fume Hood Commissioning Form
2.1 Chemical Fume Hoods2.1 Chemical Fume Hoods
Fume hoods and other capture devices are used to contain the release of toxic chemical vapors, fumes, and dusts. Bench top use of chemicals that present an inhalation hazard is strongly discouraged. Fume hoods are to be used when conducting new experiments with unknown consequences from reactions or when the potential for a fire exists.
To achieve optimum performance, the greatest personal protection and reduce energy usage when using a fume hood:
- Ensure the fume hood is working by checking the tell-tale (crepe paper hanging from hood sash) and air monitoring device if the hood is equipped with one. DO NOT use an improperly working fume hood.
- If the fume hood is not working properly, let other people in the lab know by hanging up a Do Not Use Sign (docx) on the hood.
- Work several inches inside the hood. This provides for the greatest amount of capture and removal of airborne contaminants. Also, do not place items on the airfoil or work with chemicals at the face of the hood.
- Do not block the baffles at the back of the hood. These allow for proper exhausting of contaminants from the hood.
- Keeping the hood sash lowered improves the performance of the fume hood by maintaining the internal vortex and containment. It also helps to conserve energy.
- Keep the fume hood sash closed all of the way whenever the fume hood is not being used. Shut the Sash!
- Do not use fume hoods to evaporate hazardous waste. Evaporating hazardous waste is illegal.
- For work involving particularly hazardous substances or chemicals that can form toxic vapors, fumes, or dusts, the hood or equipment within the hood may need to be fitted with condensers, traps, or scrubbers in order to prevent the vapors, fumes, and dusts from being released into the environment.
- Do not exhaust items, such as vacuum pumps, through the face of the fume hood as this will disrupt the airflow into the hood and may cause noncontainment. This will also not allow for the sash to be fully closed.
- As with any work involving chemicals, always practice good housekeeping and clean up all chemical spills immediately. Be sure to wash both the working surface and hood sash frequently and always maintain a clean and dry work surface that is free of clutter.
- In addition to annual fume hood inspection, face velocity testing, and dry ice capture testing, EHS also offers an online training program on the safe use of fume hoods. Additional information can be found in the Safe Fume Hood Use Guide.
2.1.1 Heating Perchloric Acid2.1.1 Heating Perchloric Acid
DO NOT use heated Perchloric acid in a regular fume hood. If heated Perchloric acid is used in a regular fume hood (without a wash down function), shock sensitive metallic perchlorate crystals can form inside the duct work, and could result in causing an explosion during maintenance work on the ventilation system. Use of heated Perchloric acid requires a special perchloric acid fume hood with a wash down function. If you suspect your fume hood has perchlorate contamination or would like more information on perchloric acid fume hoods, then contact askEHS.
Laboratory groups who use these hoods shall document standard procedures for the frequency and length of running the washdown system. In some cases, this may be with every use. This procedure shall also include how often a complete cleaning of the hood surfaces is required in order to ensure that dust and splatter on the inside of the hood, that may contain perchlorate salts, do not build up.
2.1.2 Fume Hood Inspection and Testing Program2.1.2 Fume Hood Inspection and Testing Program
EHS and Facilities and Campus Services share the responsibility for the annual testing and inspection of fume hoods on campus. After each inspection, an inspection sticker is affixed to the fume hood. If a hood is found to be unacceptable, a warning sign indicating the hood did not pass inspection and should not be used is fixed to the sash. This information will be provided to the building coordinator who will follow through with the repair and arrangements with other laboratories for the use of a different hood.
If your fume hood does not have an inspection sticker or if the existing inspection sticker on your fume hood indicates a year or more has passed since the hood was last inspected or for other questions please see the EHS page.
Fume hood testing and inspection consists of the following:
- The face velocity will be tested for compliance with American National Standards Institute (ANSI) and American Industrial Hygiene Association (AIHA) standard Z9.5;
- A visual performance inspection for compliance with the ANSI/American Society of Heating, Refrigerating, and Air Conditioning Engineers (ASHRAE) standard 110;
- Verification of proper room pressurization;
- A score will be taken based on the proper use and cleanliness of the hood.
- The higher the Hood Housekeeping Score, the more serious the concern.
|Hood Housekeeping Score (HHS)||Reason for Concern|
|2||Hood on, used for a single chemical process or well organized multiple purposes|
|3||Hood on, but empty or being used for storage|
|4||Hood on, crowded or used for competing multiple chemical uses|
|5||Hood on and contamination evident|
2.1.3 Installation of New Fume Hoods2.1.3 Installation of New Fume Hoods
Installation of a new fume hood requires careful planning and knowledge of the existing building ventilation systems and capabilities. Improperly installed fume hoods or other capture devices can seriously disrupt the existing ventilation system and have a negative impact in the immediate room, other fume hoods, and the ventilation system throughout the building.
All fume hoods and other capture devices must be installed in consultation with Facilities and Campus Services and EHS. All new installations of fume hoods must comply with Cornell Design Standards and be commissioned by EHS to be included in the inspection and testing program. To request a new or relocated fume hood be commissioned please submit the following form: Fume Hood Commissioning form.
EHS can provide information regarding the selection, purchase, and inspection requirements for laminar flow clean benches, biosafety cabinets, and ductless fume hoods.
2.1.4 Removal of Existing Fume Hoods2.1.4 Removal of Existing Fume Hoods
Any removal of fume hoods and capture devices requires prior consultation with your Building Coordinator, Facilities Services, and EHS. This is necessary to ensure building ventilation systems are not affected by removal of fume hoods and capture devices, and so utility services such as electrical lines, plumbing systems, and water and gas supply lines are properly disconnected. For more information about decommissioning of fume hoods, go to the Laboratory Ventilation page.
There is an additional concern for the presence of asbestos within the fume hood itself, and potentially in any pipe insulation associated with the ductwork and/or Mercury in cup sinks. Any asbestos must be properly removed and disposed of by a certified asbestos removal company. EHS can assist laboratories with the cleanup of any Mercury contamination. Contact EHS at 607-255-8200 for more information or questions about potential asbestos or Mercury contamination. See the Lab Move Guide in the appendix for more information.
2.1.5 Maintenance Procedures For Fume Hoods2.1.5 Maintenance Procedures For Fume Hoods
Please see the Laboratory Ventilation page for reference and training material.
2.2 Other Capture Devices2.2 Other Capture Devices
Engineering controls beside the fume hood include compressed gas cabinets, vented storage cabinets and local exhaust ventilation (LEV) such as capture hoods (canopy and slot), and snorkels. These work to capture and entrain chemical vapors, fumes and dusts at the point of generation. Examples where these devices would be appropriate are welding operations, atomic absorption units, vacuum pumps, work with dry nanomaterials and many other operations in the laboratory. Installation of any of these must be in the consultation of EHS and may include an engineering design to ensure the proper connection into the ventilation systems ductwork.
2.3 Glove Boxes2.3 Glove Boxes
Glove boxes are sealed enclosures that are designed to protect the user, the process or both, by providing total isolation of the contents from the outside environment. They are usually equipped with at least one pair of gloves attached to the enclosure. The user manipulates the materials inside using the gloves. Typically, a glove box has an antechamber that is used to take materials in and out of the box.Types of Glove boxes:
- Controlled Environment (dry box) - These create oxygen and moisture free conditions by replacing the air within the box with an inert gas, such as nitrogen, argon or helium, depending on the type of materials to be worked with. A "rotary vane vacuum pump" is used to remove the atmosphere. Additional accessories may be used, such a gas purifier, to further reduce oxygen and moisture levels for particularly sensitive operations. There are 4 types of this type of glove box based on their leak tightness. Class 1 having the lowest hourly leak rate. This should be inspected by a service company during commissioning, when the gloves are changed, or when there appears to be a problem with the functioning of the glove box.
- Ventilated Glove box (filtered glove box) - These have filters, either HEPA or ultra low particulate, on the inlet and outlet ends of the box and a blower to circulate the air. These provide protection to the user through this filtration and also if the exhaust is connected to building exhaust through a thimble connection. These can have serve in cleanroom applications by reversing the airflow in the chamber to positive pressure.
Regular maintenance and inspection is essential to ensure that a glove box is adequately protecting the user, the environment and/or the product/process. Routine maintenance procedures and the frequency of inspection (or certification) should follow the manufacturers and regulatory recommendations.
There are various tests that can be performed on glove boxes, the suitability of which depends on the glove box and the application. Tests may include pressure decay (for positive pressure), rate of rise (for negative pressure), oxygen analysis, containment integrity, ventilation flow characterization, and cleanliness. The source of a leak can be identified using a Mass Spectrometer Leak Detector, ultrasound, the soap bubble method or use of an oxygen analyzer. For an in-depth discussion of glove boxes and testing, see: AGS (American Glove Box Society) 2007 Guide for gloveboxes – Third Edition. AGS-G001-2007.
Please see other references at the bottom of this page for further details.
2.4 Water Protection in Labs2.4 Water Protection in Labs
Laboratory personnel must ensure that any piece of equipment or laboratory apparatus connected to the water supply utilizes backflow protection or is connected to a faucet with a vacuum breaker. The purpose of backflow prevention and vacuum breakers is to prevent water used in an experimental process or with a piece of equipment from back flowing and contaminating the laboratory’s and building’s water supply system. Examples of situations that can result from improper backflow protection include chemical contamination and/or temperature extremes (i.e. hot water coming from a drinking water fountain).
Chapter 3 - Personal Protective EquipmentChapter 3 - Personal Protective Equipment
Personal Protective Equipment (PPE) should be considered the last line of defense in protecting laboratory personnel against chemical hazards. PPE is not a substitute for good engineering controls, administrative controls, or good work practices, and are to be used in conjunction with these controls to ensure the safety and health of university employees and students.
The OSHA Personal Protective Equipment standard, 29 CFR 1910 Subpart I has the following requirements:
- Hazard assessment and equipment selection
- Employee training
- Record keeping requirements
- Guidelines for selecting PPE
- Hazard assessment certification
EHS has developed a written Personal Protective Equipment Program in compliance with the OSHA standard. More information on PPE can be found in the OSHA Safety and Health topics page on Personal Protective Equipment. Use the OSHA Eye and Face Protection eTool along with the Cornell PPE Assessment Form found in these 2 links while conducting a hazard assessment.
3.1 Laboratory Responsibilities for Personal Protective Equipment3.1 Laboratory Responsibilities for Personal Protective Equipment
Laboratory personnel need to conduct hazard assessments of specific operations occurring in their laboratories to determine what PPE is necessary to safely carry out the operations. PPE must be made available to laboratory workers to reduce exposures to hazardous chemicals in the lab. Proper PPE includes items such as gloves, eye protection, lab coats, face shields, aprons, boots, hearing protection, etc. PPE must be readily available and most equipment is provided at no cost to the employee.
When deciding on the appropriate PPE to wear when performing any operations or experiments, a number of factors must be taken into consideration such as:
- The chemicals being used, including concentration and quantity.
- The hazards the chemicals pose.
- The routes of exposure for the chemicals.
- The material the PPE is constructed of.
- The permeation and degradation rates specific chemicals will have on the material.
- The length of time the PPE will be in contact with the chemicals.
Careful consideration should be given to the comfort and fit of PPE to ensure that it will be used by laboratory personnel.
All personal protective equipment and clothing must be maintained in a sanitary and reliable condition. Only those items that meet NIOSH (National Institute of Occupational Safety and Health) or ANSI standards should be purchased or accepted for use.
There are a number of safety equipment suppliers who sell a wide variety of personal protective equipment. Be sure to check with the Purchasing department first to find out which supplier is the Cornell preferred vendor to take advantage of discounted pricing. If you have questions about what PPE is most appropriate for your applications, then contact EHS at askEH@cornell.edu.
3.2 Training for Personal Protective Equipment3.2 Training for Personal Protective Equipment
Laboratory personnel must be trained in the selection, proper use, limitations, care, and maintenance of PPE. Training requirements can be met in a variety of ways including videos, group training sessions, and handouts. Periodic retraining should be offered to both the employees and supervisors as appropriate. Examples of topics to be covered during the training include:
- When PPE must be worn.
- What PPE is necessary to carry out a procedure or experiment.
- How to properly put on, take off, adjust, and wear PPE.
- The proper cleaning, care, maintenance, useful life, limitations, and disposal of the PPE.
As with any training sessions, PPE training must be documented, including a description of the information covered during the training session and a copy of the sign-in sheet. Training records must be kept of the names of the persons trained, the type of training provided, and the dates when training occurred. EHS will maintain records of employees who attend EHS training sessions.
Information on the specific PPE required to carry out procedures within the laboratory using hazardous chemicals must also be included in the laboratory’s Standard Operating Procedures.
It is the responsibility of the Principal Investigator or laboratory supervisor to ensure laboratory staff have received the appropriate training on the selection and use of proper PPE, that proper PPE is available and in good condition, and laboratory personnel use proper PPE when working in laboratories under their supervision.
3.3 Eye Protection3.3 Eye Protection
Eye protection is one of the most important and easiest forms of PPE to wear. Laboratory personnel must use eye protection for biological, chemical and physical hazards found in laboratories including flying particles, broken glass, molten metal, acids or caustic liquids, chemical liquids, chemical gases or vapors, or potentially injurious light radiation.
It is the responsibility of Principal Investigators and laboratory supervisors to make use of eye protection a mandatory requirement for all laboratory personnel, including visitors, working in or entering laboratories under their control.
3.3.1 Eye Protection Selection3.3.1 Eye Protection Selection
All protective eye and face devices must comply with ANSI Z87.1-2003, "American National Standard Practice for Occupational and Educational Eye and Face Protection" and be marked to identify the manufacturer. When choosing proper eye protection, be aware there are a number of different styles of eyewear that serve different functions.
Prescription Safety Eyewear
OSHA regulations require that employees who wear prescription lenses while engaged in operations that involve eye hazards shall wear eye protection that incorporates the prescription in its design, or must wear eye protection that can be worn over the prescription lenses (goggles, face shields, etc.) without disturbing the proper position of the prescription lenses or the protective lenses. Any prescription eyewear purchase must comply with ANSI Z87.1-1989.
Safety glasses provide eye protection from moderate impact and particles associated with grinding, sawing, scaling, broken glass, and minor chemical splashes, etc. Side protectors are required when there is a hazard from flying objects. Safety glasses are available in prescription form for those persons needing corrective lenses. Safety glasses do not provide adequate protection for processes that involve heavy chemical use such as stirring, pouring, or mixing. In these instances, splash goggles should be used.
Splash goggles provide adequate eye protection from many hazards, including potential chemical splash hazards, use of concentrated corrosive material, and bulk chemical transfer. Goggles are available with clear or tinted lenses, fog proofing, and vented or non-vented frames. Be aware that goggles designed for woodworking are not appropriate for working with chemicals. These types of goggles can be identified by the numerous small holes throughout the facepiece. In the event of a splash, chemicals could enter into the small holes, and result in a chemical exposure to the face. Ensure the goggles you choose are rated for use with chemicals.
Welder’s goggles provide protection from sparking, scaling, or splashing metals and harmful light rays. Lenses are impact resistant and are available in graduated lens shades. Chippers'/Grinders' goggles provide protection from flying particles. A dual protective eyecup houses impact resistant clear lenses with individual cover plates.
Face shields provide additional protection to the eyes and face when used in combination with safety glasses or splash goggles. Face shields consist of an adjustable headgear and face shield of tinted or clear lenses or a mesh wire screen. They should be used in operations when the entire face needs protection and should be worn to protect the eyes and face from flying particles, metal sparks, and chemical/biological splashes. Face shields with a mesh wire screen are not appropriate for use with chemicals. Face shields must not be used alone and are not a substitute for appropriate eyewear. Face shields should always be worn in conjunction with a primary form of eye protection such as safety glasses or goggles.
Welding shields are similar in design to face shields but offer additional protection from infrared or radiant light burns, flying sparks, metal splatter, and slag chips encountered during welding, brazing, soldering, resistance welding, bare or shielded electric arc welding, and oxyacetylene welding and cutting operations.
Equipment fitted with appropriate filter lenses must be used to protect against light radiation. Tinted and shaded lenses are not filter lenses unless they are marked or identified as such.
LASER Eye Protection
A single pair of safety glasses is not available for protection from all LASER outputs. The type of eye protection required is dependent on the spectral frequency or specific wavelength of the laser source. If you have questions on the type of eyewear that should be worn with your specific LASER, contact the LASER Safety Officer at EHS at askEHS@cornell.edu. See the LASER Hazards section for more information.
3.4 Hand Protection3.4 Hand Protection
Most accidents involving hands and arms can be classified under four main hazard categories: chemicals, abrasions, cuts, and heat/cold. Gloves must be worn whenever significant potential hazards from chemicals, cuts, lacerations, abrasions, punctures, burns, biologicals, or harmful temperature extremes are present. The proper use of hand protection can help protect from potential chemical and physical hazards. Gloves must be worn when using chemicals that are easily absorbed through the skin and/or particularly hazardous substances (such as “select carcinogens”, reproductive toxins, and substances with a high degree of acute toxicity).
All glove materials are eventually permeated by chemicals; however, they can be used safely for limited time periods if specific use and other characteristics (i.e., thickness, permeation rate, and time) are known. EHS can provide assistance with determining the resistance to chemicals of common glove materials and determining the specific type of glove material that should be worn for use with a particular chemical.
3.4.1 Selecting the Proper Gloves3.4.1 Selecting the Proper Gloves
Before working with any chemical, always read manufacturer instructions and warnings on chemical container labels and SDSs. Recommended glove types are sometimes listed in the PPE section SDSs. If the recommended glove type is not listed on the SDS, then laboratory personnel should consult with the manufacturers’ glove selection charts. These charts typically include commonly used chemicals that have been tested for the manufacturers’ different glove types. Different manufacturers use different formulations so check the glove chart of the specific manufacturer for the glove you plan to use.
If the manufacturers’ glove chart does not list the specific chemical you will be using, then call the manufacturer directly and speak with their technical representatives to determine which glove is best suited for your particular application.
It is important to know that not all chemicals or mixtures have been tested by glove manufacturers. It is especially important in these situations to contact the glove manufacturer directly.
In some cases, you may need to consider hiring a testing laboratory that specializes in determining which glove material will be most resistant to the chemical you are using. For more information, contact EHS at askEHS@cornell.edu.
Some general guidelines for glove use include:
- Wear appropriate gloves when the potential for contact with hazardous materials exists. Laboratory personnel should inspect gloves for holes, cracks, or contamination before each use. Any gloves found to be questionable should be discarded immediately.
- Gloves should be replaced periodically, depending on the frequency of use and permeability to the substance(s) handled.
- Reusable Gloves should be rinsed with soap and water and then carefully removed after use.
- Discard disposable gloves after each use and whenever they become contaminated. Do not reuse disposable gloves as this poses a risk of cross-contamination and can compromise research and health.
- Due to potential chemical contamination, which may not always be visible, gloves must be removed before leaving the laboratory. Do not wear gloves while performing common tasks such as answering the phone, grabbing a door handle, using an elevator, etc. If you are required to have a glove on to hold something when leaving a lab, remove one glove and use the ungloved hand to touch door handles, elevators, etc.
3.4.2 Double Gloving3.4.2 Double Gloving
A common practice to use with disposable gloves is “double-gloving”. This is accomplished when two pairs of gloves are worn over each other to provide a double layer of protection. If the outer glove becomes contaminated, starts to degrade, or tears open, the inner glove continues to offer protection until the gloves are removed and replaced. The best practice is to check outer gloves frequently, watching for signs of degradation (change of color, change of texture, tears, etc.). At the first sign of degradation or contamination, always remove and dispose of the contaminated disposable gloves immediately and double-glove with a new set of gloves. If the inner glove appears to have any contamination or degradation, remove both pairs of gloves, and double glove with a new pair.
Another approach to double gloving is to wear a thin disposable glove (4 mil Nitrile) under a heavier glove (8 mil Nitrile). The outer glove is the primary protective barrier while the under glove retains dexterity and acts as a secondary barrier in the event of degradation or permeation of the chemical through the outer glove. Alternately, you could wear a heavier (and usually more expensive and durable) 8 mil Nitrile glove as an under glove and wear thinner, disposable 4 mil Nitrile glove as the outer glove (which can help improve dexterity). However, remember to change the thinner outer gloves frequently.
When working with mixtures of chemicals, it may be advisable to double glove with two sets of gloves made from different materials. This method can offer protection in case the outer glove material becomes permeated by one chemical in the mixture, while allowing for enough protection until both gloves can be removed. The type of glove materials selected for this type of application will be based on the specific chemicals used as part of the mixture. Check chemical manufacturers glove selection charts first before choosing which type of glove to use.
To properly remove disposable gloves, grab the cuff of the left glove with the gloved right hand and remove the left glove. While holding the removed left glove in the palm of the gloved right hand, insert a finger under the cuff of the right glove and gently invert the right glove over the glove in the palm of your hand and dispose of them properly. Be sure to wash your hands thoroughly with soap and water after the gloves have been removed.
3.4.3 Types of Gloves3.4.3 Types of Gloves
As with protective eyewear, there are a number of different types of gloves that are available for laboratory personnel that serve different functions:
Fabric gloves are made of cotton or fabric blends and are generally used to improve grip when handling slippery objects. They also help insulate hands from mild heat or cold. These gloves are not appropriate for use with chemicals because the fabric can absorb and hold the chemical against a user’s hands, resulting in a chemical exposure.
Leather gloves are used to guard against injuries from sparks, scraping against rough surfaces, or cuts from sharp objects like broken glass. They are also used in combination with an insulated liner when working with electricity. These gloves are not appropriate for use with chemicals because the leather can absorb and hold the chemical against a user’s hands, resulting in a chemical exposure.
Metal Mesh Gloves
Metal mesh gloves are used to protect hands from accidental cuts and scratches. They are most commonly used when working with cutting tools, knives, and other sharp instruments.
Cryogenic gloves are used to protect hands from extremely cold temperatures. These gloves should be used when handling dry ice and when dispensing or working with liquid nitrogen and other cryogenic liquids.
Chemically Resistant Gloves
Chemically resistant gloves come in a wide variety of materials. The recommendations given below for the specific glove materials are based on incidental contact. Once the chemical makes contact with the gloved hand, the gloves should be removed and replaced as soon as practical. Often a glove specified for incidental contact is not suitable for extended contact, such as when the gloved hand can become covered or immersed in the chemical in use. Before selecting chemical resistant gloves, consult the glove manufacturers' recommendations or their glove selection charts, or contact EHS at askEHS@cornell.edu for more assistance.
Some general guidelines for different glove materials include:
- Natural Rubber Latex - Resistant to ketones, alcohols, caustics, and organic acids. (See note below)
- Neoprene - Resistant to mineral acids, organic acids, caustics, alcohols, and petroleum solvents.
- Nitrile - Resistant to alcohols, caustics, organic acids, and some ketones.
- Norfoil- Rated for chemicals considered highly toxic and chemicals that are easily absorbed through the skin. These gloves are chemically resistant to a wide range of materials that readily attack other glove materials. These gloves are not recommended for use with Chloroform. Common brand names include: Silver Shield by North Hand Protection, 4H by Safety4, or New Barrier by Ansell Edmont.
- Polyvinyl chloride (PVC) - Resistant to mineral acids, caustics, organic acids, and alcohols.
- Polyvinyl alcohol (PVA) - Resistant to chlorinated solvents, petroleum solvents, and aromatics.
A note about latex gloves
The use of latex gloves, especially thin, disposable exam gloves, for chemical handling is discouraged because latex offers little protection from commonly used chemicals. Latex gloves can degrade severely in minutes or seconds, when used with common lab and shop chemicals. Latex gloves also can cause an allergic reaction in a percentage of the population due to several proteins found in latex. Symptoms can include nasal, eye, or sinus irritation, hives, shortness of breath, coughing, wheezing, or unexplained shock. If any of these symptoms become apparent in personnel wearing latex gloves, discontinue using the gloves and seek medical attention immediately.The use of latex gloves is only appropriate for:
- Most biological materials.
- Nonhazardous chemicals.
- Clean room requirements.
- Medical or veterinary applications.
- Very dilute, aqueous solutions containing <1% for most hazardous chemicals or less than 0.1% of a known or suspected human carcinogen.
Staff required to wear latex gloves should receive training on the potential health effects related to latex. Hypoallergenic, non-powdered gloves should be used whenever possible. If a good substitute glove material is available, then use nonlatex gloves. A general purpose substitute for disposable latex gloves are disposable Nitrile gloves.
See the appendix for a list of recommended gloves for specific chemicals, definitions for terms used in glove selection charts, glove materials and characteristics, and a list of useful references.
3.5 Protective Clothing3.5 Protective Clothing
Protective clothing includes lab coats or other protective garments such as aprons, boots, shoe covers, Tyvek coveralls, and other items, that can be used to protect street clothing from biological or chemical contamination and splashes as well as providing additional body protection from some physical hazards.
EHS requires that Principal Investigators and laboratory supervisors prohibit the wearing of shorts and skirts in laboratories using hazardous materials (chemical, biological, and radiological) by laboratory personnel, including visitors, working in or entering laboratories under their supervision.
The following characteristics should be taken into account when choosing protective clothing:
- The specific hazard(s) and the degree of protection required, including the potential exposure to chemicals, radiation, biological materials, and physical hazards such as heat.
- The type of material the clothing is made of and its resistance to the specific hazard(s) that will be encountered.
- The comfort of the protective clothing, which impacts the acceptance and ease of use by laboratory personnel.
- Whether the clothing is disposable or reusable - which impacts cost, maintenance, and cleaning requirements.
- How quickly the clothing can be removed during an emergency. It is recommended that lab coats use snaps or other easy to remove fasteners instead of buttons.
- Laboratory personnel who are planning experiments that may require special protective clothing or have questions regarding the best protective clothing to choose for their experiment(s) should contact EHS at askEHS@cornell.edu for recommendations.
3.6 Respirators3.6 Respirators
Respirators are an effective method of protection against designated hazards when properly selected and worn. Respiratory Protection includes all NIOSH approved respirators: Filtering Facepieces (Disposable respirators, N95’s, Dust Masks), Tight Fitting Half and Full Face Respirators, and Powered Air Purifying Respirators. Engineering controls, such as dilution ventilation, fume hoods, and other devices, which capture and remove dust, vapors, fumes, and gases from the breathing zone of the user are preferred over the use of respirators in most laboratory environments.
Please see the Cornell University Respiratory Protection Program web page for more information.
3.7 Hearing Protection3.7 Hearing Protection
Employees who are exposed to hazardous levels of noise in the workplace are at risk for developing noise‐induced hearing loss. Noise‐induced hearing loss is 100 percent preventable but once acquired, hearing loss is irreversible.
Please visit the Cornell Noise and Hearing Conservation Program web page for more information.
3.8 Foot Protection3.8 Foot Protection
Laboratory personnel (and other personnel) must wear foot protection at all times in laboratories, laboratory support areas, and other areas with chemical, biological and physical hazards are present. Laboratory personnel should not wear sandals or similar types of perforated or open toes shoes whenever working with or around hazardous chemicals or physical hazards. This is due to the potential exposure to toxic chemicals and the potential associated with physical hazards such as dropping pieces of equipment or broken glass being present. In general, shoes should be comfortable, and leather shoes are preferable to cloth shoes due to the better chemical resistance of leather compared to cloth. Leather shoes also tend to absorb fewer chemicals than cloth shoes. However, leather shoes are not designed for long term exposure to direct contact with chemicals. In such instances, chemically resistant rubber boots are necessary.
EHS strongly encourages Principal Investigators and laboratory supervisors to require the use of closed toed shoes for all laboratory personnel, including visitors, working in or entering laboratories and laboratory support areas under their supervision.
In some cases, the use of steel-toed shoes may be appropriate when heavy equipment or other items are involved. Chemically resistant boots or shoe covers may be required when working with large quantities of chemicals and the potential exists for large spills to occur. Cornell University sponsors a “Shoe-Mobile” every three months on campus for campus personnel to purchase a variety of steel-toed shoes for both work AND personal use at discounted prices. Contact EHS at askEHS@cornell.edu for more information on chemically resistant boots, or to find out when the “Shoe-Mobile” will be on campus.
Chapter 4 - Administrative ControlsChapter 4 - Administrative Controls
Administrative controls include policies and procedures that result in providing proper guidance for safe laboratory work practices and set the standard for behavior within the laboratory. Once developed, administrative controls must be implemented and adhered to by all personnel working in the laboratory.
Colleges and departments are responsible for developing policies and written guidelines to ensure laboratory workers are protected against exposure to hazardous chemicals as outlined in the OSHA Laboratory Standard and physical hazards that may be present, including the development of a written Chemical Hygiene Plan or adoption of this Laboratory Safety Manual.
It is the responsibility of the Principal Investigator and laboratory supervisor to ensure that personnel working in laboratories under their supervision are informed and follow laboratory specific, departmental, and campus wide policies and procedures related to laboratory safety – such as the guidelines and requirements covered in this Laboratory Safety Manual.
- OSHA Lab Standard
- How to write an SOP - Sample 1
- How to Prepare an SOP Form - Sample 2
- Chemical User Authorization Form
- Office of Risk Management and Insurance
- Office of Sponsored Programs
- Form 10
- University Policy 2.3 - Smoking
- Energy Star Website
- Energy Efficiency and Renewable Energy
- Cornell Preferred Vendors
- Self inspection checklist and explanation key
- What to do during an OSHA inspection
- What to do during an EPA inspection
- EHS Online Training Programs
- OSHA Sanitation Standard
- Blank SOP Form - Sample 1
- Blank SOP Form - Sample 2
- Example of completed SOPs
- Office of University Counsel
- Compliance certifications
- University Policy 6.5 - University Volunteers
- University Policy 2.8 - Pets on Campus
- EPA Pollution Prevention website for Green products
- Cornell University Energy Saving Tips
- EHS Signs and Labels webpage
- CU Design Standards
- Emergency Shower/Eyewash Commissioning Form
- Safe Fume Hood Use Guide
- Research Area Space Registration webpage
- CDC Lists of Biological Diseases and Chemical Agents
4.1 Standard Operating Procedures4.1 Standard Operating Procedures
The OSHA Laboratory Standard requires that Chemical Hygiene Plans include specific elements and measures to ensure employee protection in the laboratory. One such requirement is Standard Operating Procedures (SOPs) “relevant to safety and health considerations to be followed when laboratory work involves the use of hazardous chemicals.”
SOPs can be stand-alone documents or supplemental information included as part of research notebooks, experiment documentation, or research proposals. The requirement for SOPs is to ensure a process is in place to document and addresses relevant health and safety issues as part of every experiment.
At a minimum, SOPs should include details such as:
- The chemicals involved and their hazards.
- Special hazards and circumstances.
- Use of engineering controls (such as fume hoods).
- Required PPE.
- Spill response measures.
- Waste disposal procedures.
- Decontamination procedures.
- Description of how to perform the experiment or operation.
While the OSHA Laboratory Standard specifies the requirement for SOPs for work involving hazardous chemicals, laboratories should also develop SOPs for use with any piece of equipment or operation that may pose any physical hazards. Examples include:
- Safe use and considerations of LASERSs.
- Use of cryogenic liquids and fill procedures.
- Connecting regulators to gas cylinders and cylinder change outs.
- Use of equipment with high voltage.
SOPs do not need to be lengthy dissertations and it is perfectly acceptable to point laboratory personnel to other sources of information. Some examples of what to include as part of SOPs are:
EHS can assist laboratories with developing general and specific SOPs. Due to the variety of research and the large number of laboratories on the Cornell campus, it is the responsibility of each laboratory, department and college to ensure that SOPs are developed and the practices and procedures are adequate to protect lab workers who use hazardous chemicals.
Examples of Standard Operating Procedures and blank SOP templates include:
4.2 Form 104.2 Form 10
The Office of Sponsored Programs (OSP) administers awards for sponsored research, instruction, and extension projects at Cornell. The Form 10 is the internal academic approval form for sponsored programs. The form should be completed by the Principal Investigator and submitted with all proposals. Of particular note for laboratory personnel, compliance certifications are required for the following areas:
- Human Subjects
- Animal Use
- Recombinant DNA
- Genetically Modified Organisms
- Biological Agents and Toxins
- Hazardous Materials
As part of the Form 10 process, EHS will be notified upon submittal of the Form 10 to OSP when one or more of the following compliance items have been checked: animals, rDNA, GMOs, radiation, hazardous materials, or biological agents or toxins. Once EHS receives the Form 10, staff members within EHS will contact the Principal Investigator listed to discuss general aspects of the grant proposal and to ensure health and safety aspects have been taken into account. During the review process, EHS staff members will identify any special issues that may need to be addressed to ensure compliance with state or federal regulatory requirements.
More information can be obtained from the Office of Sponsored Programs website – Form 10: Internal Academic Approval of Sponsored Programs.
4.3 Procedural Controls4.3 Procedural Controls
Procedural controls incorporate best management practices for working in a laboratory. These practices serve not only to protect the health and safety of personnel, but are a common sense way of increasing productivity in a laboratory. Through implementation of good practices, laboratories can expect an increase in the efficient use of valuable lab space, in the reliability of experiments due to less potential contamination, and an increase in the awareness of health and safety issues by laboratory personnel. Following the practices outlined in this Lab Safety Manual should also result in a decrease in the number of accidents, injuries, and spills. This will result in a decrease in the overall liability for the Principal Investigator, laboratory supervisor, and the University. Procedural controls are fundamental to instilling safe work behaviors and helping to create a culture of safety within the laboratory environment.
4.4 Housekeeping4.4 Housekeeping
Housekeeping refers to the general condition and appearance of a laboratory and includes:
- Keeping all areas of the lab free of clutter, trash, extraneous equipment, and unused chemical containers. Areas within the lab that should be addressed include benches, hoods, refrigerators, cabinets, chemical storage cabinets, sinks, trash cans, etc.
- Keep all containers of chemicals closed when not in use.
- Cleaning up all chemicals spills immediately, regardless if the chemical is hazardous or not. When cleaning up a chemical spill, look for any splashes that may have resulted on nearby equipment, cabinets, doors, and counter tops. For more information on cleaning up spills, see the Chemical Spill Procedures section.
- Keeping areas around emergency equipment and devices clean and free of clutter. This includes items such as eyewash/emergency showers, electric power panels, fire extinguishers, and spill cleanup supplies.
- Keeping a minimum of three feet of clearance (as required by fire codes) between benches and equipment. Exits must be clear of obstacles and tripping hazards such as bottles, boxes, equipment, electric cords, etc. Combustible materials may not be stored in exits (including corridors and stairways), exit enclosures, boiler rooms, mechanical rooms, or electrical equipment rooms.
- When storing items overhead, keep heavier and bulkier items closer to the floor. New York State (NYS) Building Code prohibits the storage of combustible material (such as paper, boxes, plastics, etc.) within 24" of the ceiling in unsprinklered rooms. In sprinklered rooms, All storage, including both combustible and non-combustible materials, must be kept at least 18” below the level of the sprinkler head deflectors to ensure that fire sprinkler coverage is not impeded.
- Always use a stepladder when reaching for overhead items, do not stand on chairs or countertops. If you do not have a stepladder available, then contact your Building Coordinator.
In summary, good housekeeping has obvious health and safety benefits and can have a positive mental effect on laboratory personnel who work in a clean environment, which can lead to increased productivity. Also keep in mind that during an inspection by a state or federal regulatory agency, the general condition of the laboratory observed in the first few minutes of the inspection (the housekeeping of the lab) can have a significant impact (positive or negative) on the rest of the inspection process.
4.5 Personal Hygiene4.5 Personal Hygiene
Good chemical hygiene practices include the use of personal protective equipment (PPE) and good personal hygiene habits. Although PPE can offer a barrier of protection against chemicals and biological materials, good personal hygiene habits are essential to prevent chemical exposure, even when using PPE.Some general guidelines that should always be followed include:
- Do not eat, drink, chew gum, or apply cosmetics in a lab or other area where chemicals are used.
- Do not store food or drink in refrigerators that are used to store chemicals.
- Do not ever try starting a siphon or pipette by mouth, doing so can result in ingestion of chemicals or inhalation of chemical vapors. Always use a pipette aid or suction bulb to start a siphon.
- Always confine long hair, loose clothing, and jewelry.
- Wear a lab coat when working with hazardous materials.
- Shorts and sandals should not be worn in a lab when anyone is using corrosives or other chemicals that present a skin contact hazard or where the potential for physical hazards such as dropping pieces of equipment or broken glass are present.
- Remove laboratory coats, gloves, and other PPE immediately when chemical contamination occurs. Failure to do so could result in chemical exposure.
- After removing contaminated PPE, be sure to wash any affected skin areas with soap and water for at least 15 minutes.
- Always remove lab coats, scrubs, gloves, and other PPE before leaving the lab. Do not wear lab coats, scrubs, or other PPE (especially gloves) in areas outside the lab, particularly not in areas where food and drink are served, or other public areas.
- Always wash hands with soap and water after removing gloves and before leaving the lab or using items such as the phone, turning doorknobs, or using an elevator.
- Always wash lab coats separately from personal clothing. Be sure to identify contaminated lab coats to commercial laundry facilities to help protect their workers by placing the contaminated lab coat in a separate plastic bag and clearly identifying the bag with a note or label indicating the lab coat is contaminated.
- Smoking is prohibited in all lab areas at Cornell.
4.6 Eating, Drinking, and Applying Cosmetics in the Lab4.6 Eating, Drinking, and Applying Cosmetics in the Lab
Chemical and other toxic materials exposure can occur through ingestion of food or drink contaminated with these items. This type of contamination can occur when food or drinks are brought into a lab or when food or drinks are stored in refrigerators, freezers, or cabinets with laboratory materials. When this occurs, it is possible for the food or drink to absorb chemical vapors or other contaminants and thus lead to an exposure when the food or drink is consumed. Eating or drinking in areas exposed to toxic materials is prohibited by the OSHA Sanitation Standard, 29 CFR 1910.141(g)(2).
A similar principle of potential chemical or toxic materials exposure holds true with regard to the application of cosmetics (make-up, hand lotion, etc.) in a laboratory setting. In this instance, the cosmetics have the ability of absorbing chemical vapors, dusts, and mists from the air and when applied to the skin and result in skin exposure.
To prevent exposure to hazardous chemicals or toxic materials through ingestion, do not eat, drink, chew gum, or apply cosmetics in areas where these materials are used.
Wash your hands thoroughly after using any chemicals or other laboratory materials, even if you were wearing gloves, and especially before eating or drinking.
To help promote awareness, refrigerators and freezers should be properly labeled:
- Refrigerators for the storage of food should be labeled, “Food Only, No Chemicals” or “No Chemicals or Samples”.
- Refrigerators used for the storage of chemicals should be labeled “Chemicals Only, No Food”.
Free refrigerator labels are available from the EHS Signs and Labels webpage.
Keep in mind that some exposures can result in immediate effects (acute exposure) while other effects may not be seen for some time despite repeated exposure (chronic exposure). Consuming food or drink or applying cosmetics in the lab can result in both types of exposure.
4.7 Working Alone4.7 Working Alone
Whenever possible, laboratory personnel should avoid working alone when conducting research, especially when experiments involve hazardous substances and procedures. Laboratories should establish specific guidelines and standard operating procedures specifying when working alone is not allowed and develop notification procedures when working alone occurs. All work to be performed by someone working alone, and the monitoring system that is established, must be approved in advance by the Principal Investigator or laboratory supervisor. Check with your DSR to see if your department has specific requirements for working alone.
If a laboratory person determines it is necessary to work alone, consideration should be given to notifying someone else in the area – in an adjacent room, another lab on the same floor, or a lab on a different floor. It is recommended that a “buddy system” be established for regular, routine checks on personnel working alone, such as every 15 – 30 minutes, to ensure no accidents have occurred. This could be accomplished by physically walking to the room where the lab worker is or through the use of a phone. If the person working alone is doing highly hazardous work, then the person checking on the lab worker should not enter same room. A system of visual checks should be established to indicate there are no problems or to determine if help is needed.
In the event of an emergency that requires the buddy to leave prior to the completion of an experiment involving highly hazardous chemicals, the buddy should notify Cornell Police at 607-255-1111 of the name, location, and end time of the experiment involved. The buddy should also notify the person conducting the experiment. The person conducting the experiment should make an effort to complete the experiment in a safe manner and notify Cornell Police upon completion of the experiment.
Examples of activities where working alone would be permissible include:
- Office work such as writing papers, calculations, computer work, and reading.
- Housekeeping activities such as general cleaning, reorganization of supplies or equipment, etc., as long as no moving of large quantities of chemicals is involved.
- Assembly or modification of laboratory apparatus when no chemical, electrical, or other physical hazards are present.
- Routine lab functions which are part of a standard operating procedure which has been demonstrated to be safe and not involve hazardous materials.
Examples of activities where working using a “buddy system” should be considered include:
- Experiments involving toxic or otherwise hazardous chemicals, especially poison inhalation hazards.
- Experiments involving high-pressure equipment.
- Experiments involving large quantities of cryogenic materials.
- Experiments involving work with unstable (explosives) materials.
- Experiments involving Class 3b or 4 Lasers.
- Transfer of large quantities of flammable materials, acids, bases, and other hazardous materials.
- Changing out compressed gas cylinders containing hazardous materials.
4.8 Phones in Labs4.8 Phones in Labs
All labs are strongly recommended to have a means of communication in the event of an emergency. This can include a phone or cell phone (if service is available) or two-way radio within the lab or access to a central phone located in the hallway. If a phone is not available within the lab, it is advisable to post a sign and/or map indicating where the nearest phone is located.
4.9 Unattended Operations4.9 Unattended Operations
Whenever it is necessary to have unattended operations occurring in a lab, it is important to ensure safeguards are put into place in the event of an emergency. Laboratory personnel are strongly encouraged to adhere to the following guidelines when it is necessary to carry out unattended operations.
For unattended operations involving highly hazardous materials, a light should be left on and an appropriate warning/explanation sign should be placed on the laboratory door, or in a conspicuous place that could be easily seen without putting someone else in danger in the event of an emergency.
The warning sign should list the following information:
- The nature of the experiment in progress.
- The chemicals in use.
- Hazards present (electrical, heat, etc.)
- The name of the person conducting the experiment and a contact number. A secondary name and contact number is also recommended.
When setting up an experiment that will be left unattended, try to take into account potential incidents that could occur if something went wrong. For example:
- Use secondary containment such as trays to contain any spills that may occur.
- Use safety shields and keep the chemical hood sash down low to contain chemicals and glass in case an explosion occurs.
- Remove any chemicals or equipment that are not necessary for the experiment or items that could potentially react with the chemicals or other materials being used in the experiment.
- Whenever possible, use automatic shutoff devices to prevent accidents such as loss of cooling water shutoff, over-temperature shut off, etc.
- Use emergency power outlets for those pieces of equipment that could be negatively affected in the event electric service or other city utilities are interrupted.
4.10 Access to Laboratories4.10 Access to Laboratories
Access to Cornell University laboratories, workshops and other work areas housing hazardous materials or machinery is restricted to Cornell faculty, staff, students, or other persons on official business.
4.10.1 Visitors and Children in Labs4.10.1 Visitors and Children in Labs
Due to the potential hazards and liability issues, other persons, in particular children under the age of 16 are not permitted in hazardous work areas, with the exception of University-sanctioned activity, e.g., tours, open houses, or other University related business as authorized by the Principal Investigator or laboratory supervisor. In these instances, all children under the age of 16 must be under careful and continuous supervision. Check with your DSR to see if your department has specific procedures or policies in place for visitors.
4.10.2 Volunteers in Labs4.10.2 Volunteers in Labs
Volunteers in labs are restricted by the University’s Volunteer Policy. Please review this policy for guidance and/or consult with the University’s Office of Risk Management and Insurance for more information.
4.10.3 Visiting Scientists and Other Similar Users4.10.3 Visiting Scientists and Other Similar Users
There are potential risks associated with allowing access to labs and equipment by visiting scientists. These risks include: theft or questions of ownership for intellectual property, bodily injury, and property damage. Colleges and units should verify that all users of the lab have the required safety and health training prior to allowing access to the lab and/or specialized equipment. It is the user’s responsibility to have or obtain the appropriate training. Units are advised to consult with the University’s Office of Risk Management and Insurance and/or Office of University Counsel to obtain contracts and agreements to minimize risks associated with the use of labs and equipment by visiting scientists and others.
4.10.4 Pets in Labs4.10.4 Pets in Labs
The Cornell University Policy 2.8 – Pets on Campus, specifically states that pets are prohibited “from university-controlled buildings, except for those animals that are specifically exempted by this policy. In addition, while on university-controlled property, animals must be attended and restrained at all times.
4.11 Chemical Purchasing4.11 Chemical Purchasing
Before ordering new chemicals, search your existing inventories and use those chemicals currently in stock. An accurate and up-to-date chemical inventory can help to minimize purchase of chemicals already on hand and can facilitate acquisition of Safety Data Sheets (SDS). Cornell has an institutional subscription to the Vertere chemical inventory system that can assist with maintaining a chemical inventory. If you are interested in learning more about the Vertere system, contact askEHS@cornell.edu.
If it is necessary to purchase new chemicals, laboratory personnel should order the smallest size necessary to carry out the experiment. Avoid ordering extra quantities because the chemical “might be needed in the future”. Try to take advantage of chemical vendors “Just-In-Time” delivery rather than stockpiling chemicals in your lab. Before ordering chemicals, be sure to check Cornell purchasing guidelines for preferred vendors and pricing.
Some chemical purchases may require special approval or permits, such as the Drug Enforcement Administration (DEA) controlled substances and/or listed chemicals; Alcohol, Tobacco, and Firearms (ATF) listed substances; select agents or particularly hazardous substances. Building and fire codes restrict the amount of hazardous materials that can be stored in any one room, floor, and building at any given time. For more information, contact askEHS@cornell.edu.
4.12 Ordering New Equipment4.12 Ordering New Equipment
Whenever large pieces of equipment are planned to be purchased and installed in laboratories, especially equipment that is required to be hooked up to building utility services such as electric, water, or gas, laboratory personnel must first consult with Facilities Engineering, EHS, and the appropriate PDC shops to ensure the building has the necessary resources to support the new piece of equipment. Lab personnel should not assume they can purchase equipment first and then expect the building to be able to handle the service requirements later. By preplanning and communicating well in advance with appropriate campus groups (such as Facilities Engineering and EHS), any potential issues can be identified ahead of time, which in turn will help make the transition to getting new pieces of equipment up and running quickly after the purchase is made.
Additionally, as with installation of fume hoods, certain pieces of equipment require special installation due to their potential impact on the rest of the building ventilation system and utilities, and cannot be hooked up by laboratory personnel, building managers, or private contractors without first consulting with Facilities Engineering and EHS. Laboratory personnel are strongly encouraged to be proactive and to consult with the appropriate departments ahead of time, before purchasing new pieces of large equipment.
Laboratory personnel are strongly encouraged, as responsible campus members, to give consideration to purchasing “Energy Star” energy efficient pieces of equipment to help conserve natural resources and long-term operating costs. When discussing purchases of equipment with vendors and equipment manufacturers, ask about what “Energy Star” alternatives they carry. For more information, see Energy Conservation in Laboratories.
Before ordering new equipment, check the Cornell purchasing guidelines for preferred vendors and pricing.
4.13 Work Orders and Ticket Requests4.13 Work Orders and Ticket Requests
In the event of a maintenance issue or if repairs are needed to equipment, laboratory personnel should first consult with their Building Coordinator, who will submit the appropriate paperwork with Customer Service to have repairs initiated. Please note that due to NYS building codes and liability issues, laboratory personnel must not try to repair utility services (such as electrical, plumbing, or gas issues) by themselves. These repairs must be handled by qualified personnel only.
Whenever maintenance workers will be working on your hood system or in your laboratory, please remove all chemicals, laboratory apparatus, and equipment from the area requiring maintenance work. Ensure the work area is clean and inform the maintenance workers of any potential hazards present in the near vicinity either verbally or by leaving a sign with the appropriate information.
4.14 Changes in Lab Occupancy4.14 Changes in Lab Occupancy
Changes in laboratory occupancies can occur when faculty retire, new faculty come to campus, new lab staff are hired, students graduate or leave for another university, or when facility renovations take place. When changes in lab occupancy occur, it is important to address any potential issues BEFORE the occupants leave.
Failure to address the change in occupancy can result in:
- Old, unlabeled chemicals, samples, or hazardous waste being left behind in refrigerators, freezers, and cabinets.
- Valuable furniture or equipment being moved or thrown away.
- Unknown chemical spills or contamination being present.
- These issues can result in costly remediation efforts and wasted resources for both the department and the University.
If you are planning to leave your laboratory or if you know of a research group or students that are planning to leave, there are a few simple steps that can be followed to ensure a smooth transition:
- Notify your department chairperson, lab supervisor, and DSR well in advance of the planned move.
- Ensure all chemical containers are properly labeled.
- Properly dispose of any hazardous and chemical waste left in the laboratory.
- Ensure all chemical spills and contamination has been cleaned up.
- Review the EHS Lab Move Guide.
4.15 Laboratory Design and Construction4.15 Laboratory Design and Construction
Project Managers planning construction or renovation of a laboratory should include EHS at design scoping phase of the project.
Research Safety lab design elements include:
Americans with Disability Act compliance: This may involve the determination of types and location of fume hoods, safety showers and eyewashes, autoclaves, sinks and other lab equipment.
Chemical inventory: A chemical inventory that includes quantity and intended usage will be expected at Scoping phase of all laboratory projects.
The chemical inventory must also include all cryogenic liquids and compressed gases. A risk assessment must be conducted to determine the need for a gas cabinet, low exhaust diffusers, oxygen monitoring, emergency ventilation shut-off. Specialized storage may be needed for certain chemicals such as acutely toxic, air/water reactive, and controlled substances. Examples include double lock boxes, flammable or explosion proof refrigerators or storage cabinets dedicated to a specific hazard class.
Emergency Showers and Eyewashes: These are required in laboratories that use chemicals that may spill or splash onto anyone working in the lab. Showers are required for volumes greater than a gallon.
Laboratory Ventilation: Air exchange rates are determined by EHS and are based on volatile chemical use, hazardous gas use, and/or biological agents. Other drivers are heat and the exhaust requirements for equipment in the room.
Volatile and corrosive chemicals produce airborne contaminants that must be controlled when in storage as well as when in use. This could include ventilated storage cabinets, a sufficient number of fume hoods, or other enclosures and local exhaust.
The design will also include directional general ventilation in lieu of local exhaust ventilation where it is not possible for the lab population to utilize, such as in Gross Anatomy in the Veterinary College.
Directional airflow relative to the hallway and adjacent rooms must be discussed for all laboratory projects.
Fume hood and other exposure control devices: The type and quantities of fume hoods, location in the lab suite, and face velocity (with minimum velocities) must be determined at design. Local exhaust may include snorkels, canopy, heat extracting hoods, or other types of tables.
Specialty hoods: Wet benches (for corrosive chemical use), solvent benches, and perchloric acid fume hoods (with the washdown feature) must be discussed with EHS to determine requirement and special considerations.
Biological Safety: A review of the nature of proposed research biological agents, including plants or animals, must happen to determine room design elements. The researcher must register with the Institutional Biosafety Committee for work with disease causing agents in humans, animals, and plants. Approval may also be required by the IACUC and the Institutional Review Board.
Specific design elements include:
- Type and location of biosafety cabinets;
- Types, location, and electrical requirements for ULT freezers, incubator, growth chambers, and other floor or benchtop equipment;
- Floor materials, sink locations, and environmental conditions for the room or a support lab.
Radiation Safety: Users of ionizing radiation require a Cornell Permit. Most non-ionizing radiation equipment requires registration. The following programs are managed by the Radiation Safety group who must be contacted by project managers during the design scoping phase when any proposal involves the use of this equipment. There are specific room requirements.
Lasers: Class 3B and 4 lasers. The facility housing these must be designed to mitigate the laser hazards. These must be registered for use on campus.
Magnet/UV/RF: Magnetic fields are produced by this equipment can be hazardous.
Radiation Producing Equipment includes X-ray equipment (x-ray diffraction, x-ray fluorescence, x-ray radiography, ion implanters, electron microscopes).
Radioactive materials, including unsealed materials and sealed sources.
References (Use most recent versions):
1) ANSI Z9.5 Laboratory Ventilation
3) ANSI Z358.1 Emergency Eyewash and Shower Equipment
4.16 Ventilation Rates4.16 Ventilation Rates
As part of energy conservation measures, ventilation rates for laboratories are determined based on the occupancy and the type of research being conducted. Please see the Laboratory Ventilation page on this website for further information about the program.
If the function of a room changes due to new researchers coming to campus notify EHS through askEHS@cornell.edu about the change. EHS will then verify if the ventilation rate for a given room is appropriate for the type of research being conducted.
4.16.1 Room Air Pressure in Labs4.16.1 Room Air Pressure in Labs
Research laboratories should be negative to the hallways and offices. When positive air pressure occurs, more air is being supplied to the room than what is being removed by the fume hood or general exhaust. This can result in air from the laboratory (including chemical vapors and dusts) being blown out into the hallway outside of the lab and chemical odors permeating the hallways and surrounding rooms.
- Ensure the entrance door to the laboratory is closed.
- If you notice odors that seem to be escaping from a lab, then please contact your Building Coordinator for assistance.
4.17 Energy Conservation in Laboratories4.17 Energy Conservation in Laboratories
Laboratories are energy intensive facilities, consuming many times the energy use of the average non-lab academic buildings. Laboratories use large quantities of air for ventilation and fume hoods; electricity to operate fans, lighting, and specialized lab equipment; and large quantities of water and process chilled water. Some laboratory facilities also use substantial quantities of natural gas.
There are a number of things that lab occupants can do to reduce the overall consumption of energy:
- Turn off the room lights.
- When possible turn off electrical equipment when not in use.
- Use timers to turn other pieces of equipment on and off automatically.
- Unplug equipment when not in use.
- Turn off your computer’s monitor when not in use. The monitor consumes over half of the energy used by the average computer. Put your computer and monitor into "sleep" mode after 10 minutes and cut power use nearly to zero.
- Keep the sash closed on your fume hood. This promotes both energy conservation and safety.
- If you would like to temporarily turn off your fume hood, please contact your building coordinator.
- Rooms that are too hot or too cool may be due to faulty thermostats or other controls that are malfunctioning or have drifted from set points, resulting in wasted energy as well as uncomfortable conditions for you. If you experience these problems, then contact your Building Coordinator for assistance.
- Report drips of water from sink taps, chilled water connections or Reverse Osmosis (RO) faucets.
- Clean out and consolidate freezers and refrigerators at least once per year.
- Set refrigerator and freezer temperatures at necessary levels instead of the lowest set point the equipment can achieve.
- Consolidate incubator use and freezer storage to minimize the number of appliances used.
- Use shades and blinds as provided to help keep your space cool on sunny days. The shade can reduce the amount of cooling required in a south or west facing room by over 30%.
- Use electric smart strips to minimize electricity used by items on the strip (EcoStrips).
- Discourage the use of space heaters.
- Develop maintenance schedules for scientific equipment, such as cleaning compressor coils on cooling devices, to extend the device's life and maintain its energy efficiency.
4.18 Green Labs4.18 Green Labs
Green chemistry, also known as sustainable chemistry, is the design of chemical products and processes that reduce or eliminate the use or generation of hazardous substances. Green chemistry applies across the life cycle of a chemical product, including its design, manufacture, and use.
- Substitute hazardous chemicals with less hazardous alternatives or chemicals that are drain disposable
- Redesign chemical processes to reduce hazardous chemical use and/or exposure to technicians
- Maintain an ongoing inventory of chemicals and dispose of outdated chemicals on a regular basis.
Find additional information on Greening your lab and certifying your lab as a Green Lab:
- EPA Pollution Prevention website for Green products
- CU Green Your Lab
- American Chemical Society Green Chemistry Institute
Additional information on energy conservation for both work and home can be found on the Department of Energy's website: Energy Efficiency and Renewable Energy. Find energy savings tips for the home, office, and lab by going to the Utilities Department Energy Saving Tips webpage.
4.19 Research Area Inspections4.19 Research Area Inspections
Laboratories and other research areas are regulated by OSHA laboratory safety standards and general industry regulations, EPA and DEC hazardous waste regulations, DOH regulations, NFPA life and fire safety standards, and building codes. Additionally, accreditation and granting agencies such as CDC, NIH, and USDA are increasing scrutiny over researchers and their compliance with state and federal laws. To assist researchers to be in compliance with these regulations and standards, Environmental Health & Safety (EHS) will conduct required inspections of all campus research areas.
The purpose of the inspections is to assist responsible faculty and staff members in identifying and correcting potential regulatory compliance issues or other issues that could affect granting activities, and identify potential health and safety hazards that could pose an unreasonable risk to laboratory personnel, students, and the campus community. To facilitate the correction of deficient items, a corrective action process has been implemented and will be tracked. EHS will schedule inspections by working with college-level contacts, Department Safety Representatives, Building Coordinators and staff throughout the colleges, departments, and buildings.
Research areas are strongly encouraged to conduct their own self inspections prior to EHS conducting an inspection of their research area to address any potential issues before the EHS inspection and to provide a training opportunity for research staff. To facilitate the self inspection process, EHS is providing research areas with the following self inspection checklist and explanation key which identify the same topics covered during an EHS inspection.
4.19.1 Self Inspections4.19.1 Self Inspections
An important part of any research safety program is implementation of self inspections. Self inspections provide a number of useful benefits and further help to create a culture of safety within the lab. Benefits of self inspections include:
- Raising the level of awareness of laboratory personnel and determining the level of compliance with state and federal regulations.
- Identifying and addressing any potential issues before an inspection by a state or federal regulatory agency.
- Providing an opportunity for lab specific training by identifying potential issues within the lab and then training lab personnel to look for these issues.
- Serving as a regular health and safety check of laboratory facilities.
- Serving as an outlet for faculty, staff, and student concerns.
- EHS recommends the following frequency for self inspections:
- On a daily basis lab personnel should maintain good housekeeping within their lab.
- Informal weekly lab walkthroughs or “Friday afternoon cleanups”.
- Ideally, self inspections should occur once per month. These could include participation of research staff, DSRs, and/or safety committee members, and use of an inspection checklist.
- At least once per semester research personnel should perform a formal self inspection utilizing the EHS self-inspection checklist and explanation key.
The benefits of conducting inspections of laboratories on a regular basis cannot be overstated. In addition to providing for a healthier and safer work environment, lab inspections can reduce legal liability by identifying potential issues, and training lab personnel to look for and correct potential issues.
4.19.2 Inspections by Regulatory Agencies4.19.2 Inspections by Regulatory Agencies
Inspections by state and federal regulatory agencies can occur at any time and can result in citations and significant fires for the university. The best way to be prepared for these inspections is to understand what regulations apply to your area and what you need to do to comply with those regulations. You can obtain this information from resources such as this Laboratory Safety Manual, by conducting your own self inspections, and by calling EHS at 607-255-8200. You can find additional information on the EHS web page on what to do during an OSHA inspection and what to do during an EPA inspection.
If a state or federal inspector shows up in your work area unescorted, ask them to please wait and contact EHS immediately at 607-255-8200.
4.20 Research Area Space Registration Using HASP4.20 Research Area Space Registration Using HASP
All research spaces are required to be registered with the Department of Environmental Health and Safety (EHS) using the online Hazard Assessment Signage Program (HASP). While Facilities Services Space Inventory accounts for room function and type, Research Area Space Registration accounts for hazards present in rooms to facilitate regulatory compliance, identify training requirements, communicate hazards, and improve emergency response.
Research areas must be reviewed and registered on an annual basis, when roster or hazard information changes and upon notification by EHS. The registration process consists of using the online HASP tool and entering contact information, hazards present in the room, risk levels of hazards, access limitations, warning messages, and emergency response information. The entire process of completing HASP for one room should only take a few minutes for each room. Once a research area has been initially entered into the system, annual updates can be completed in less time. Only certain research area rooms types are required to be registered. The list of space inventory room types of interest that will be required to complete annual Research Space Registration using the online HASP tool can be found on the Research Area Space Registration webpage.
The following outlines responsibilities for implementation of Research Area Space Registration using HASP:
The Department of Environmental Health and Safety is responsible for:
- Providing information and assistance with the HASP system.
- Granting access to the users for their locations within the HASP system.
- Providing guidance and assistance on the identification of hazard types.
- Providing information, training, notifications, reports, and updates from the information provided.
Deans and Department Chairpersons are responsible for:
- Ensuring that research areas within their departments and units are registered in a timely manner upon notification by EHS and updated annually.
Principal Investigators and Research Area Supervisors are responsible for:
- Registering (or designating someone to register) their research areas using HASP in a timely manner upon notification by EHS.
- Updating their Research Area Space Registration using HASP when any new hazards or significant change of existing hazards occurs.
- Updating their Research Area Space Registration annually in a timely manner upon notification by EHS.
EHS will work with Department Safety Representatives (DSRs) and Building Coordinators to facilitate the implementation of Research Area Space Registration. Before getting started, persons completing the registration process (DSRs, Building Coordinators, Principal Investigators, research staff, or other college and department designated personnel) will first need to be given authorization to the HASP system for their organization, building, department, and/or specific rooms. To obtain authorization for specific areas, please contact askEHS@cornell.edu. For more information on using the online HASP tool and how to get started with the program, see the online training program for using HASP.
4.21 Laboratory Security4.21 Laboratory Security
Laboratories need to take specific actions in order to provide security against theft of highly hazardous materials, valuable equipment, and to ensure compliance with state and federal regulations. EHS encourages each unit (college, department, and research group) to review and develop procedures to ensure the security of all hazardous materials in their area of responsibility.
Many laboratories already implement various means of security, including requirements to lock up controlled substances, syringes and needles, and radioactive materials. EHS recommends you review and assess the hazardous materials in your laboratory and consider security issues in protecting those materials. The intent is to minimize the risk of theft, especially targeting the five-minute window when the lab is left unattended.
4.21.1 Security Guidelines4.21.1 Security Guidelines
The following are guidelines designed to minimize opportunities for intentional removal of any hazardous materials from your laboratory:
- Recognize that laboratory security is related to, but different from laboratory safety. Security is preventing intrusion into the laboratory and the theft of equipment or materials from the lab.
- Develop a site-specific security policy. Make an assessment of your laboratory area for hazardous materials and particular security issues. Then develop and implement lab security procedures for your lab group and train lab group members on security procedures and assign responsibilities.
- Control access to areas where hazardous chemicals are used and stored. Limit laboratory access to only those individuals who need to be in the lab and restrict off-hours access only to individuals authorized by the Principal Investigator.
- Be sure to lock freezers, refrigerators, storage cabinets, and other containers where stocks of biological agents, hazardous chemicals, or radioactive materials are stored when they are not in direct view of workers (for example, when located in unattended storage areas).
- Do not leave hazardous materials unattended or unsecured at any time. Most importantly, close and lock laboratory doors when no one is present.
Chapter 5 - Emergency PreparationChapter 5 - Emergency Preparation
IN CASE OF AN EMERGENCY:
Ithaca Campus: CALL 911 from any campus phone or dial 607-255-1111 from any cell phone, or off campus phone to reach Cornell University Police. Consult the Emergency Action Guide for more information.
Outlying Facilities: CALL 911 or follow your facility emergency response procedure. Consult the Emergency Action Guide for more information.
Emergencies can occur at any time, without warning. Careful planning, with an emphasis on safety, can help members of the Cornell community handle crises and emergencies with appropriate responses, and could save lives. Every member of the Cornell community shares responsibility for emergency preparedness. Unit heads are responsible for ensuring that their units have emergency plans in place, and that all persons – including faculty, staff and students – are familiar with those emergency plans. Unit heads are also responsible for assigning emergency preparedness and response duties to appropriate staff members.
- OSHA Medical Services and First Aid Standard
- OSHA Permissible Exposure Limits Topics Webpage
- University Policy 8.3 - Emergency Planning
- EHS Safety Education Catalog
- Eyewash Testing Sheet
- OSHA Access to employee exposure and medical records standard
- Cornell University Injury/Illness Exposure Reporting
- Cornell University Emergency Plan webpage
- EHS Online Training Programs
- Emergency Shower and Eyewash Commissioning Form
5.1 Cornell Emergency Plan5.1 Cornell Emergency Plan
Cornell University organizes, coordinates, and directs available resources toward an effective response to, and recovery from emergencies under the Cornell Emergency Program. The effectiveness of this effort is dependent on the development of a comprehensive central plan and individual college/unit plans. The university, therefore, expects colleges, divisions and individual departments to develop detailed emergency plans. This policy includes a chain of command establishing the authority and responsibilities of campus officials and staff members, and requires that colleges, divisions, and individual departments designate emergency coordinators with the authority to make modifications in emergency procedures and to commit resources for emergency preparedness and recovery, as necessary.
5.1.1 Unit Emergency Planning5.1.1 Unit Emergency Planning
The Emergency Planning and Recovery system provides tools and guidance to colleges, divisions, and individual departments in developing detailed unit emergency plans. Policy 8.3 – Emergency Planning requires that every college and major administrative unit have designated emergency coordinators. The emergency coordinator should be a full-time member of the administrative team, and preferably an experienced employee who is thoroughly familiar with College/ Administrative Unit and University procedures. Knowledge of programs and physical facilities in their College/ Administrative Unit is also imperative. This person will coordinate their College's/Administrative Unit emergency plan as well as oversee that the College's/Administrative Unit each prepares a unit emergency plan. Each College/ Administrative Unit leader (e.g. Dean or Vice President) is responsible for designating an Emergency Coordinator. This person is responsible for gathering and communicating emergency information, coordinating and assisting in evacuations, maintaining emergency response forms and other emergency plan materials.
The Emergency Coordinator must be familiar with the programs and physical facilities, and should be a person with the management experience and authority to:
- Collaborate with departments to develop and maintain the information in the Unit Emergency Plan.
- Recruit a core "Emergency Preparedness Committee" that represents staff, faculty, and principal investigators from the unit's major sub-divisions or locations.
- Arrange related staff safety education and training.
- Coordinate resources for emergency preparedness and recovery.
- Purchase emergency supplies and equipment.
- Be ready to support managers during an emergency incident (and be called back to Cornell if necessary).
- Be ready to help prepare post-emergency impact summaries and insurance claims.
5.1.2 Fire Safety Plans5.1.2 Fire Safety Plans
Fire safety planning is very important to the Cornell University community. The University has developed campus-wide procedures to follow in the event of an emergency that must be posted in elevator lobbies, stairwells, and assembly spaces. More information about fire safety plans can be found on the EHS Fire Safety web page.
5.2 Emergency Evacuation Procedures5.2 Emergency Evacuation Procedures
Information about Emergency Evacuation Procedures can be found in the Fire Safety Plan document.
Evacuation of Persons with Disabilities
See the Disability Escape Route Planning to prepare for Evacuation of Persons with Disabilities.
5.3 Emergency Procedures5.3 Emergency Procedures
Emergencies can include both fire and non-fire emergencies. Fires are an "expected" emergency in all lab situations and almost all lab staff are trained on emergency steps in the event of a fire. “Non-fire” emergencies can include:
- Loss of electricity, heat, AC, water or other essential utilities.
- Failure of mechanical equipment such as HVAC systems and emergency generators.
- Flooding, tornadoes, earthquakes, or other natural disasters.
- Nearby chemical releases of hazardous materials to the environment (from the lab down the hall or a ruptured tank car one-half mile away).
- Terrorist actions or civil unrest.
5.3.1 Laboratory Emergency Shutdown Procedures5.3.1 Laboratory Emergency Shutdown Procedures
Each laboratory facility should develop a non-fire emergency plan or incorporate non-fire emergencies into a master emergency response plan. Employees must be trained on the contents of the plan and how to respond in a non-fire emergency. Cornell EHS has devised a set of simple steps for the shutdown of labs in non-fire emergency situations. These and other steps, based on the requirements of the facility, should be included in the emergency response plan of each unit or facility. This list is by no means complete, but it gives laboratory personnel simple steps to ensure a safe lab shutdown.
- Close fume hood sashes.
- Be certain that the caps are on all bottles of chemicals.
- Turn off all non-essential electrical devices. Leave refrigerators and freezers on and make sure the doors are closed. Check the disconnects of large LASERs, radio frequency generators, etc. It may be necessary to check to ensure that essential equipment is plugged in to the power receptacles supplied by the emergency generator (usually orange or red).
- Turn off all gas cylinders at the tank valves. Note: If a low flow of an inert gas is being used to "blanket" a reactive compound or mixture, then the lab worker may want to leave the flow of gas on. This should be part of a pre-approved, written, posted standard operating procedure for this material or process.
- Check all cryogenic vacuum traps (Nitrogen, Carbon dioxide, and solvent). The evaporation of trapped materials may cause dangerous conditions. Check all containers of cryogenic liquids to ensure that they are vented to prevent the buildup of internal pressure.
- Check all pressure, temperature, air, or moisture sensitive materials and equipment. This includes vacuum work, distillations, glove boxes used for airless/moistureless reactions, and all reactions in progress. Terminate all reactions that are in progress, based on the known scope of the emergency.
- If experimental animals are in use, special precautions may need to be taken to secure those areas such as emergency power, alternative ventilation, etc.
- All non-essential staff/students must leave the building. Depending on the nature of the emergency, some staff may need to stay behind to facilitate the start-up of essential equipment once the lab is reopened.
- It is important to remember that some equipment does not shut down automatically – such as large cryogenic magnets, sources of radioactivity, and other pieces of equipment. Be sure to check any special operating procedures for your equipment before an emergency occurs.
5.3.2 Medical Emergency Procedures5.3.2 Medical Emergency Procedures
Call 911 (or 607-255-1111 from a cell phone in Ithaca Campus) in any emergency that requires immediate police, fire or medical response to preserve a life.
- Protect the victim from further injury or harm by removing any persistent threat to the victim or by removing the victim to a safe place if needed, however do not move the victim unnecessarily. Do not delay in obtaining trained medical assistance if it is safe to do so.
- Notify Cornell Police of the location, nature and extent of the injury by calling 911 or using a Blue Light or Emergency Telephone in Ithaca Campus. Always call from a safe location.
- Provide first aid until help arrives if you have appropriate training and equipment, and it is safe to do so.
- Send someone outside to escort emergency responders to the appropriate location, if possible.
5.3.3 First Aid Kits5.3.3 First Aid Kits
Individual departments and units are not required to maintain first aid kits in work spaces within the campus buildings. As indicated in OSHA (29 CFR 1910.151) and cited in the ANSI standard (ANSI Z308.1-2003) if medical attention can be reached within a reasonable time, or distance, to rely on the professionals and make that part of an emergency plan. Cornell’s EHS department has fully trained emergency responders on call 24 hours a day, 7 days a week in Ithaca Campus. Injured personnel are encouraged to take advantage of this service by calling 911 from a campus phone or 607-255-1111 from a cell phone.
Outlying facilities, follow your facility Emergency Plan.
If you choose to have a first aid kit in your work space, then there are some additional requirements to address. The first aid content list should be viewed mainly as a starting point for an organization’s first aid kit, as many workplaces have job-specific risks that should be addressed on a case-by-case basis with the addition of products necessary to meet those unique needs. There needs to be a responsible person in your work space that is trained - with their contact information posted on the kit. The kit should be maintained and complete at all times. An Injury/Illness Exposure Reporting should be completed when a first aid kit is used due to an injury/illness in a Cornell University laboratory.
The appropriate Training is provided live by EHS. Course # EHS 5360 – AHA Heartsaver First Aid.
The ANSI Standard lists the following minimum fill requirements for a first aid kit:
- 1 - Absorbent compress, 4 x 8 in. minimum
- 5 yard Adhesive Tape
- 10 - Antiseptic applications, 0.14 fl.oz. each
- 1 - Triangular bandage, 40 x 40 x 56 in. minimum
- 16 - Adhesive Bandages, 1 x 3 inch
- 2 - Pair medical exam gloves
- 4 - Sterile pads, 3 x 3 in. minimum
- 6 - Burn treatment applications, 1/32 oz. each
5.3.4 Fire or Explosion Emergency Procedures5.3.4 Fire or Explosion Emergency Procedures
All fires must be reported to Cornell Police, including those that have been extinguished. Do not hesitate to activate the fire alarm if you discover smoke or fire. Outlying facilities, all fires including those that have been extinguished, must be reported to Cornell EHS (607-255-8200). Consult the Emergency Action Guide for outlying facility site specific procedures.
- Alert people in the immediate area of the fire and evacuate the room.
- Confine the fire by closing doors as you leave the room.
- Initiate a full building evacuation by activating the closest fire alarm pull station as you are exiting the building.
- Notify Cornell Police of the location and size of the fire by calling 911 from a campus phone, or 607-255-1111 from a cell phone or off campus phone, or using a Blue Light or Emergency Telephone. Always call from a safe location.
- Evacuate the building using the Emergency Evacuation Procedure. Do not use elevators to evacuate unless directed to do so by emergency responders.
- Notify emergency responders of the location, nature and size of the fire once you are outside.
If you have been trained and it is safe to do so, you may attempt to extinguish the fire with a portable fire extinguisher. Attempt to extinguish only small fires and make sure you have a clear escape path. If you have not been trained to use a fire extinguisher you must evacuate the area.
If clothing is on fire:
- Stop - Drop to the ground or floor and Roll to smother flames.
- Drench with water from a safety shower or other source.
- Seek medical attention for all burns and injuries.
5.3.5 Fire Extinguishers5.3.5 Fire Extinguishers
- All fire extinguishers are inspected monthly and maintained by Facilities and Campus Services in main campus and some outlying facilities. Other outlying facilities, a local contractor is provided.
- Laboratory personnel should perform regular visual checks (minimum on a monthly basis) to ensure fire extinguishers present in their labs are fully charged. For those fire extinguishers with a readout dial, labs only need to ensure the indicator arrow on the readout dial is within the green zone. If the indicator arrow is on either side of the green zone, which indicates a problem, then call EHS at 607-255-8200 to have the fire extinguisher replaced.
- Any fire extinguisher that has been used at all, even if it wasn’t fully discharged, needs to be reported to EHS so a replacement fire extinguisher can be provided in its place.
- The University Fire Marshal's Office conducts onsite Fire Extinguisher Training (course #5300) and can be scheduled through CU Learn.
5.3.6 Power Outage Procedures5.3.6 Power Outage Procedures
- Assess the extent of the outage in the unit's area.
- At outlying facilities, refer to your facility Emergency Action Guide.
- Report the outage to Cornell Customer Service Center at 607-255-5322.
- Assist other building occupants to move to safe locations. Loss of power to fume hoods may require the evacuation of laboratories and surrounding areas.
- Implement the unit's power outage plan. Evaluate the unit's work areas for hazards created by a power outage. Secure hazardous materials. Take actions to preserve human and animal safety and health. Take actions to preserve research.
- Turn off and/or unplug non-essential electrical equipment, computer equipment and appliances. Keep refrigerators and freezers closed throughout the outage to help keep contents cold.
- If needed, open windows (in mild weather) for additional light and ventilation (this is not always advisable in BSL2 labs).
5.4 Chemical Spill Procedures5.4 Chemical Spill Procedures
When a chemical spill occurs, it is necessary to take prompt and appropriate action. The type of response to a spill will depend on the quantity of the chemical spilled and the severity of the hazards associated with the chemical. The first action to take is to alert others in your lab or work area that a spill has occurred. Then you must determine if you can safely clean up the spill yourself.
At remote facilities, refer to your facility chemical spill response procedure.
Many chemical spills can be safely cleaned up by laboratory staff without the help of EHS. Only attempt to clean up incidental spills if you are trained and have the proper spill cleanup materials available. Note: The following advice is intended for spills that occur within a University building. A release to the outside environment may require the University file a report with the EPA. Calling Cornell Police at 607 255-1111 or 911 will initiate this determination by Environment, Health and Safety (EHS).
5.4.1 Incidental Spills5.4.1 Incidental Spills
A spill is considered incidental if the criteria below are met:
- The spill is a small quantity of a known chemical.
- No gases or vapors are present that require respiratory protection.
- You have the materials and equipment needed to clean up the spill.
- You have the necessary proper personal protective (PPE) equipment available.
- You understand the hazards posed by the spilled chemical.
- You know how to clean up the spill.
- You feel comfortable cleaning up the spill.
- You know how to properly dispose of spill cleanup procedures.
- You have a procedure to replace items used during the spill cleanup.
184.108.40.206 Incidental Spill Cleanup Procedures220.127.116.11 Incidental Spill Cleanup Procedures
- Notify other people in the area that a spill has occurred. Prevent others from coming in contact with the spill (i.e. walking through the spilled chemical). The first priority is to always protect yourself and others.
- Put on the Proper Personal Protective Equipment (PPE) such as goggles, gloves, etc. before beginning cleanup. Do not unnecessarily expose yourself to the chemical.
- Stop the source of the spill if possible, and if safe to do so.
- Try to prevent spilled chemicals from entering waterways by building a dike around access points (sink, cup sinks, and floor drains inside and storm drains outside) with absorbent material if you can safely do so.
- Use the appropriate absorbent material for liquid spills (detailed in the following section).
- Slowly add absorbent material on and around the spill and allow the chemical to absorb. Apply enough absorbent to completely cover the spilled liquid.
- Sweep up the absorbed spill from the outside towards the middle.
- Scoop up and deposit in a leak-proof container.
- For acid and base spills, transfer the absorbed materials to a sink, and complete the neutralization prior to drain disposal.
- For absorbed hazardous chemicals, label the container and dispose of through the hazardous waste managementprogram.
- If possible, mark the area of the spill on the floor with chalk.
- Wash the contaminated surface with soapy water. If the spilled chemical is highly toxic, collect the rinse water for proper disposal.
- Report the spill to your supervisor.
- Restock any spill clean up supplies that you may have used from any spill kits.
5.4.2 Spill Absorbent Materials5.4.2 Spill Absorbent Materials
For acid spills (except Hydrofluoric acid):
- Sodium carbonate
- Sodium bicarbonate (baking soda)
- Calcium carbonate
- Calcium bicarbonate
- Do not use absorbent clay for acid spills
For Hydrofluoric acid (HF) spills:
- Use Calcium carbonate or Calcium bicarbonate to tightly bind the fluoride ion.
For liquid base spills:
- Use Sodium bicarbonate to lower the pH sufficiently for drain disposal.
For oil spills:
- Use ground corn cobs (SlikQwik), vermiculite, or absorbent clay (kitty litter).
For most acqueous solutions:
- Use ground corn cobs (SlikQwik)
For most organic liquid spills:
- Use ground corn cobs (SlikQwik). If the liquid is flammable, be sure to use an excess of SlikQwik.
For oxidizing liquids:
- Use absorbent clay, vermiculite, or some other nonreactive absorbent material. Do not use SlikQwik or paper towels. Note: Most nitrate solutions are not sufficiently oxidizing for this requirement.
For mercury spills:
- Do not dispose of mercury or mercury contaminated spill debris in the regular trash or down the drain.
- There is no absorbent material available. Physical removal processes are best for removing and collecting mercury.
- If you need help collecting Mercury from a spill, contact EHS spill responders by calling (607) 255-1111 or 911. Note: While powdered sulfur will help reduce mercury vapors, the sulfur greatly complicates the spill cleanup.
5.4.3 Spill Kits5.4.3 Spill Kits
While commercially available spill kits are available from a number of safety supply vendors, laboratory personnel can assemble their own spill kits to properly clean up chemicals specific to their laboratory. Whether commercially purchased or made in-house, it is expected that all laboratories have access to an appropriately stocked spill kit to address the hazards in the space. Colleges and departments should give serious consideration to distributing basic spill kits to all laboratories within their units.
A useful spill kit can be assembled using a 2.5 or 5 gallon bucket containing the following absorbent materials. Stock only the absorbents appropriate for your space. Each container of absorbent must be labeled as to what it contains and what type of spills it can be used for.
Spill kit absorbent material:
- 1-5 lbs of ground corn cobs (SlikQwik) – for most aqueous and organic liquid spills.
- 1-5 lbs of absorbent clay (kitty litter) - for oils or oxidizing liquids.
- 1-5 lbs of Sodium bicarbonate - for liquid acid and base spills.
- 1-5 lbs of Calcium carbonate or Calcium bicarbonate - for HF spills.
Equipment in the spill kit could include:
- Wisk broom and dust pan (available at home improvement stores)
- pH paper
- 1 gallon and 5 gallon bags - for collection of spill cleanup material
- Small and large Ziploc bags – for collection of spill cleanup material or to enclose leaking bottles/containers.
- Safety goggles
- Thick and thin Nitrile gloves
- Hazardous waste labels
The spill kit should be clearly labeled as “SPILL KIT”, with a list of the contents posted on or in the kit. This list should include information about restocking the kit after use and where to obtain restocking materials.
Laboratory personnel must also be properly trained on:
- How to determine if they can or should clean up the spill, or if they should call 911 or EHS at 607-255-8200.
- Where the spill kit will be kept within the laboratory.
- What items are in the kit and where replacement items can be obtained.
- How to use the items in the kit properly.
- How to clean up the different types of chemical spills.
- How to dispose of spill cleanup material.
Environmental Health and Safety can provide assistance in assembling spill kits for laboratories and offers a training class on “Cleaning Up Small Spills". More information can be obtained by contacting Environmental Health and Safety at 607-255-8200.
5.4.4 Major Spills5.4.4 Major Spills
A major spill is any chemical spill for which the researcher determines they need outside assistance to safely clean up a spill. EHS is activated to assist with spill cleanup whenever Cornell Police are notified of a spill by calling 911 from a campus phone or 607-255-1111 from a cell phone or off campus phone.
18.104.22.168 Major Spill Cleanup Procedures22.214.171.124 Major Spill Cleanup Procedures
When a spill occurs that you are not capable of handling:
- Alert people in the immediate area of the spill and evacuate the room.
- If an explosion hazard is present, do not unplug, or turn electrical equipment on or off – doing so can result in a spark and ignition source.
- Confine the hazard by closing doors as you leave the room.
- Use eyewash or safety showers as needed to rinse spilled chemicals off people or yourself.
- Evacuate any nearby rooms that may be affected. If the hazard will affect the entire building, then evacuate the entire building by pulling the fire alarm.
- Notify Cornell Police by calling 911 or using a Blue Light or Emergency Telephone. Always call from a safe location.
Be prepared to provide Cornell Police with the following information:
- Where the spill occurred (building and room number).
- If there are there any injuries and if medical attention is needed.
- The identity of the spilled material(s) - be prepared to spell out the chemical names.
- The approximate amount of material spilled.
- How the spill occurred (if you know).
- Any immediate actions you took.
- Who first observed the spill and the approximate time it occurred.
- Where you will meet emergency responders, or provide a call back number (if available).
- Once outside, notify emergency responders of the location, nature and size of the spill. Isolate contaminated persons and protect yourself and others from chemical exposure.
5.5 Emergency Eyewash and Showers5.5 Emergency Eyewash and Showers
All laboratories using hazardous chemicals, particularly corrosive chemicals, must have access to an eyewash and/or an emergency shower as per the OSHA standard 29 CFR 1910.151 – Medical Services and First Aid. The ANSI Standard Z358.1-2014 - Emergency Eyewash and Shower Equipment provides additional guidance by stating that emergency eyewash and/or emergency showers be readily accessible, free of obstructions and within 10 seconds from the hazard. The ANSI standard also outlines specific requirements related to flow requirements, use of tempered water, inspection and testing frequencies, and training of laboratory personnel in the proper use of this important piece of emergency equipment.
5.5.1 Testing and Inspection of Emergency Eyewash and Showers5.5.1 Testing and Inspection of Emergency Eyewash and Showers
The ANSI Standard provides guidance by stating that plumbed emergency eyewash and safety showers should be activated weekly to verify proper operation and inspected annually. Regular activation (weekly flushing) ensures the units are operating properly, helps to keep the units free of clutter, and helps prevent the growth of bacteria within the plumbing lines, which can cause eye infections. It is recommended to allow the water to run for at least 3 minutes. EHS strongly encourages laboratories to post an “Eyewash Testing Sheet” near the eyewash to keep track and document that weekly activation is occurring.
Laboratories are responsible for activating eyewashes in their spaces and ensuring that access to eyewashes and emergency showers are kept free of clutter and ensuring the eyewash nozzle dust covers are kept in place. If nozzle dust covers are not kept on the eyewash nozzles, dust or other particles can clog the nozzles and effect water flow. This could result in dust or other particles being forced into the eyes when the eyewash is used.
Report any malfunctioning eyewashes and emergency showers to your Building Coordinator to have the unit repaired. If either the emergency shower or eyewash is not working properly, posta Do Not Use sign on the unit to alert others.
EHS performs free annual inspections of eyewashes and emergency showers. EHS will test units for compliance with ANSI Z358.1-2014 including:
- Test the water flow for proper quantity, spray pattern, and good water quality.
- Ensure the unit is the proper height from the floor.
- Ensure the unit is not obstructed.
- Ensure the unit has a tempering valve (if the unit does not have a tempering valve, this will be identified as a recommended repair in the inspection report).
- Ensure valves are working properly.
- Ensure signs are posted.
- Ensure the unit is free of corrosion.
Area Managers or delegates may conduct this annual inspection. Completion of the CULearn module #2720 explaining how to conduct the inspection and reporting process. In addition to affirming completion of the module, SEW Area Tester Guidelines can be used as a reference.
5.5.2 Installation of New Emergency Eyewash and Showers5.5.2 Installation of New Emergency Eyewash and Showers
As with installation of other safety equipment, all new eyewashes and emergency showers must be installed in consultation with Facilities Engineering, EHS, and the appropriate campus service shops. All new installations or eyewashes and emergency showers must comply with CU Design Standard 15430 – Safety Showers and Eyewashes. Before EHS will commission any new emergency shower or eyewash, the project manager or designated representative must complete an Emergency Shower and Eyewash Commissioning Form and submit it to the program manager.
5.5.3 Maintenance Procedures For Emergency Eyewash and Showers5.5.3 Maintenance Procedures For Emergency Eyewash and Showers
The following documents provide information and maintenance procedures for working on emergency eyewashes and showers:
5.5.4 Using Emergency Eyewash and Showers5.5.4 Using Emergency Eyewash and Showers
Preplan your experiments and include emergency procedures. At minimum identify the locations of the nearest emergency shower and eyewash before working with hazardous chemicals.
In the event of an emergency (chemical spill or splash) where an eyewash or emergency shower is needed, follow these procedures:
- If you get a chemical in your eyes, yell for help if someone else is in the lab.
- Immediately go to the nearest eyewash and push the activation handle all the way on.
- Put your eyes or other exposed area in the stream of water and begin flushing.
- Open your eyelids with you fingers and roll your eyeballs around to get maximum irrigation of the eyes.
- Keep flushing for at least 15 minutes or until help arrives. The importance of flushing the eyes first for at least 15 minutes cannot be overstated! For accidents involving Hydrofluoric acid, follow the special Hydrofluoric acid precautions.
- If you are alone, call 911 after you have finished flushing your eyes for at least 15 minutes.
- Seek medical attention.
- Complete an Injury/Illness Exposure Report.
If someone else in the lab needs to use an eyewash, assist them to the eyewash, activate the eyewash for them, and help them get started flushing their eyes using the procedures above and then call 911. After calling 911, go back to assist the person using the eyewash and continue flushing for 15 minutes or until help arrives and have the person seek medical attention.
- If you get chemical contamination on your skin resulting from an accident, yell for help if someone else is in the lab.
- Immediately go to the nearest emergency shower and pull the activation handle.
- Once under the stream of water, begin removing your clothing to wash off all chemicals. In some instances, clothing may not be removed, (although it is best to remove contaminated clothing), it is more important to flush away chemical contamination.
- Keep flushing for at least 15 minutes or until help arrives. The importance of flushing for at least 15 minutes cannot be overstated! If you spill Hydrofluoric acid on yourself, follow the special Hydrofluoric acid precautions.
- If you are alone, call 911 after you have finished flushing for at least 15 minutes.
- Seek medical attention.
- Complete an Injury/Illness Exposure Reporting.
If someone else in the lab needs to use an emergency shower (and it is safe for you to do so), assist them to the emergency shower, activate the shower for them, and help them get started flushing using the procedures above and then call 911. After calling 911, go back to assist the person using the shower and continue flushing for 15 minutes or until help arrives and have the person seek medical attention.
5.6 Injury/Illness/Exposure Reporting5.6 Injury/Illness/Exposure Reporting
All accidents and injuries, no matter how minor, are required to be reported to University officials through the injury/illness/exposure reporting system. The employee, supervisor, department head or a designated individual within the department must complete all sections of this form as soon as possible and ideally within 24 hours after the injury/illness/exposure is first reported. The online Injury/Illness reporting system can be accessed through the EHS webpage – Cornell University Injury/Illness/Exposure Reporting.
5.7 Medical Consultations5.7 Medical Consultations
When a chemical exposure occurs, medical consultations and medical examinations will be made available to laboratory workers who work with hazardous chemicals as required. All work related medical examinations and consultations will be performed by or under the direct supervision of a licensed physician and will be provided at no cost to the employee without loss of pay, and at a reasonable time, through the Cornell Health.
The opportunity to receive medical attention, including any follow up examinations, will be provided to employees who work with hazardous chemicals under the following circumstances:
- Whenever an employee develops signs or symptoms associated with a hazardous chemical to which the employee may have been exposed in the laboratory.
- Where airborne exposure monitoring reveals an exposure level routinely above the action level (or in the absence of an action level, the Permissible Exposure Limit) for an OSHA regulated substance for which there are exposure monitoring and medical surveillance requirements. Action level means the airborne concentration of a specific chemical, identified by OSHA, and calculated as an 8-hour time weighted average (TWA).
- Whenever an event such as a spill, leak, explosion or other occurrence takes place and results in the likelihood of a hazardous exposure. Upon such an event, the affected employee shall be provided an opportunity for a medical consultation. The consultation shall be for the purpose of determining the need for a medical examination.
More information on action levels and Permissible Exposure Limits can be found on the OSHA Health and Safety topics page – Permissible Exposure Limits.
5.7.1 Information Provided to the Physician5.7.1 Information Provided to the Physician
The physician shall be provided with the following information:
- The identity of the hazardous chemical(s) to which the employee may have been exposed. Such information can be found in the Safety Data Sheet (SDS) for the chemical(s).
- A description of the conditions under which the exposure occurred including quantitative exposure data, if available.
- A description of the signs and symptoms of exposure that the employee is experiencing, if any.
5.7.2 The Physician’s Written Opinion5.7.2 The Physician’s Written Opinion
The physician’s written opinion for the consultation or examination shall include:
- The results of the medical examination and any associated tests.
- Any medical condition that may be revealed in the course of the examination, which may place the employee at increased risk as a result of exposure to a hazardous workplace.
- A statement that the employee has been informed by the physician of the results of the consultation or medical examination and any medical condition that may require further examination or treatment.
- The written opinion shall not reveal specific findings of diagnoses unrelated to the occupational exposure.
All records of medical consultations, examinations, tests, or written opinions shall be maintained at Cornell Health in accordance with 29 CFR 1910.1020 - Access to employee exposure and medical records. The Cornell Health (607-255-5155) is located at 10 Central Avenue. Exposure monitoring records of contaminate levels in laboratories will be maintained at EHS office at 395 Pine Tree Road, Suite 210. For more information, contact EHS at 607-255-8200.
Chapter 6 - Info and TrainingChapter 6 - Info and Training
Federal and state laws and Cornell University policy require all laboratory workers to receive Laboratory Safety and Chemical Waste Disposal training and be informed of the potential health and safety risks that may be present in their workplace. Documentation must be maintained to demonstrate that such training was provided and received. In order to assist laboratory personnel comply with this requirement, laboratory safety training must be obtained either through EHS (classroom or web-based sessions) or documented as having been received from an alternative source.
The OSHA Laboratory Standard requires employers to provide employees with information and training to ensure they are apprised of the hazards of chemicals present in their work area. The Laboratory Standard goes on to state that such information shall be provided at the time of an employee’s initial assignment to a work area where hazardous chemicals are present and prior to assignments involving new exposure situations.
As per the OSHA Laboratory Standard, information that must be provided to employees includes:
- The contents of the Laboratory Standard and its appendices (Appendix A and Appendix B) shall be made available to employees.
- The location and availability of the employer's Chemical Hygiene Plan.
- The permissible exposure limits for OSHA regulated substances or recommended exposure limits for other hazardous chemicals where there is no applicable OSHA standard.
- Signs and symptoms associated with exposures to hazardous chemicals used in the laboratory.
- The location and availability of identified reference materials listing the hazards, safe handling, storage and disposal of hazardous chemicals found in the laboratory including, but not limited to, SDSs received from the chemical supplier.
- The Laboratory Standard goes on to state this training shall include:
- Methods and observations that may be used to detect the presence or release of a hazardous chemical.
- The physical and health hazards of chemicals in the work area.
- The measures employees can take to protect themselves from these hazards, including specific procedures the employer has implemented to protect employees from exposure to hazardous chemicals, such as appropriate work practices, emergency procedures, and PPE to be used.
The employee shall be trained on the applicable details of the employer’s written Chemical Hygiene Plan.
While the OSHA Laboratory Standard is specific to working with hazardous chemicals, as per the University Health & Safety Policy 8.6, laboratory employees must also be provided with the proper training and information related to the other health and physical hazards that can be found in their work environment, including the hazards described within this Laboratory Safety Manual.
- OSHA Lab Standard OSHA
- OSHA Lab Standard Appendix B
- University Environment, Health & Safety Policy 8.6
- EHS Training Webpage
- Laboratory Certificate Program webpage
- Office of Sponsored Programs
- Lab Standard Appendix A
- OSHA Permissible Exposure Limits Topics Webpage
- EHS Online Training Programs
- Research Administration Certification Program
- Division of Financial Affairs
6.1 Training Options6.1 Training Options
Principal Investigators and laboratory supervisors have a number of options available to them to ensure laboratory employees under their supervision have received proper training. These options include:
- Training programs provided by EHS
- Training programs provided by outside vendors
- In-house training programs (provided by the Principal Investigator or laboratory supervisor)
- Training manuals and booklets
- Training videos
- Web-based training modules
The keys to any training programs are:
- The instructor providing the training is technically qualified to provide training on the particular subject.
- The training program(s) address the hazards present in the laboratory and describe ways employees can protect themselves.
- The training program and attendance must be documented using a sign-in sheet and these records must be readily available and accessible upon request.
- Training sessions do not have to be hours or half-day sessions, they can be short, 15 minute, half hour, or how ever long it takes to achieve the training objectives.
EHS Training Programs
EHS offers a number of training programs on a regular basis – such as the monthly “Laboratory Safety Training” – and offers a number of programs “Upon Request”. For any “Upon Request” training class, EHS can come to your building or laboratory and provide the training program for your laboratory group. All EHS provided training programs and attendance sheets are kept on file at the EHS office and entered into the Cornell Learning Management System.
Outside Vendor Training Programs
Principal Investigators and laboratory supervisors can provide training programs to their employees through contracts with outside training companies or product vendors. A number of vendors are willing to provide free training programs upon request. If using an outside company or vendor, be sure to ask for documentation including training content, date of training, copies of handouts, and the sign-in sheet. All of this documentation must be kept on file.
In-House Training Programs
In-house training can include department provided training, and training by Principal Investigators and laboratory supervisors. Training sessions can be stand-alone classes, on-the-job training, or short (15 minute) trainings incorporated as part of a laboratory group meeting. The key is to make sure the training is documented with a sign-in sheet.
Training Manuals and Booklets
Principal Investigators and laboratory supervisors can utilize training manuals, booklets, webpage downloads, etc., as part of an ongoing training program by simply having laboratory staff review the material, be given an opportunity to ask any questions, and sign off that they read and understood the material.
Principal Investigators and laboratory supervisors can make use of videos to supplement training of their employees. As with any training, it is important to document the training took place by using a sign-in sheet. When videos are used, the training sign-in sheet should have the date, time, location, and name and running time of the video, in addition to signatures of those people who watched the video.
Web Based Training
Web-based trainings are offered through CU Learn for faculty, staff and students (including undergraduates and graduates). Blackboard can be used by visitors, guests or CU affiliates (USDA, BTI).
6.2 Cornell Learning Management System6.2 Cornell Learning Management System
The Cornell Learning Management System is a database created to help employees, students, trainers, supervisors, and safety managers track safety training and keep required certifications current via the Internet. Using your Cornell University Net ID as a unique identifier, you can access different types of training information. As a user, you can view current classes being offered, register for classes, and keep a record of the training classes you have taken. With supervisor or safety manager access, you can assign specific training courses to your employees and verify their training history, in addition to other features. For information, see the EHS Training webpage.
6.3 Laboratory Safety Certificate Program6.3 Laboratory Safety Certificate Program
The Laboratory Safety Certificate Program is a voluntary program designed to encourage laboratory workers to broaden their safety knowledge by completing a variety of training programs related to laboratory safety, and to recognize and reward their accomplishment by granting a Laboratory Safety Certificate.
To qualify for a certificate, the applicant must complete a specified number of required and elective courses within a given time period. The courses may be taken as live classroom sessions or completed on-line. For more information, see the Laboratory Certificate Program webpage.
6.4 Research Administration Certification Program6.4 Research Administration Certification Program
Cornell University’s Research Administration Certification Program (RACP), developed in collaboration with a cross-campus advisory committee, and coordinated through a partnership between Office of Sponsored Programs and Division of Financial Affairs, is a professional development opportunity designed to educate Cornell staff about sponsored programs administration.
The purpose of the program is to develop and maintain a skilled cadre of research administration professionals within the university and to promote a culture of compliance and integrity. This program is recommended for staff whose work supports sponsored projects, particularly those new to the field or new to Cornell University.
The curriculum provides a high-level overview of key concepts related to sponsored programs administration at Cornell. The five day core training program, offered three times a year, is spread out over five weeks, with class one day per week (9:00 am – 4:00 pm). Participants attend lectures and use problem-based learning exercises to engage in real-world issues. In addition to the core courses, 15 hours of electives are required within one year and continuing education is also required. Those who attend all five days of RACP, achieve passing grades on exams and complete the required electives, receive certification. For more information, see the Office of Sponsored Programs - Research Administration Certification Program webpage.
Chapter 7 - Safe Chemical UseChapter 7 - Safe Chemical Use
Safe chemical use includes minimizing exposure to chemicals, proper training, understanding chemical hazards, proper labeling, proper storage and segregation, and proper transport.
- OSHA Lab Standard
- OSHA Permissible Exposure Limits Topics Webpage
- OSHA Definition of Physical Hazard
- EHS Training Webpage
- EHS Signs and Labels webpage
- Safety Data Sheets Webpage
- SDS Hyperglossary
- HMIG and HMIS
- Safety in Academic Chemistry Laboratories
- Department of Transportation (DOT) hazard class system
- OSHA Toxic and Hazardous Substances
- OSHA Definition of Health Hazard
- University Health and Safety Policy
- EHS Online Training Programs
- EHS Right-To-Know Chemical Labels
- SDS FAQ
- NFPA diamond
- OSHA SDS Form 174
- Prudent Practices in the Laboratory
- DOT training Modules
- Department of Transportation (DOT) hazard class system
7.1 Minimize Exposure to Chemicals7.1 Minimize Exposure to Chemicals
The best way laboratory personnel can protect themselves from chemical hazards is to minimize their exposure to them. In order to minimize chemical exposure:
- Substitute less hazardous chemicals in your experiments whenever possible.
- Always use the smallest possible quantity of chemical for all experiments. Consider microscale experiments and activities.
- Minimize chemical exposures to all potential routes of entry - inhalation, ingestion, skin and eye absorption, and injection through proper use of engineering controls and personal protective equipment.
- Be sure to select the proper PPE and regularly inspect it for contamination, leaks, cracks, and holes. Pay particular attention to gloves.
- Do not pipette or apply suction by mouth.
- Do not smell or taste chemicals. When it is necessary to identify a chemical’s odor, lab personnel should hold the chemical container away from their face and gently waft their hand over the container without inhaling large quantities of chemical vapor.
- Do not underestimate the risk of exposure to chemicals - even for substances of no known significant hazard.
- In order to identify potential hazards, laboratory personnel should plan out their experiments in advance. These plans should include the specific measures that will be taken to minimize exposure to all chemicals to be used, the proper positioning of equipment, and the organization of dry runs.
- Chemicals that are particularly hazardous substances require prior approval from your supervisor and special precautions to be taken.
- When working with mixtures of chemicals, laboratory personnel should assume the mixture to be more toxic than the most toxic component in the mixture.
- Consider all substances of unknown toxicity to be toxic until proven otherwise.
- Request exposure monitoring to ensure the Permissible Exposure Limits (PELs) of OSHA and the current Threshold Limit Values (TLVs) of the American Conference of Governmental Industrial Hygienists are not exceeded.
- Promptly clean up all chemicals spills regardless whether the chemical is considered hazardous or nonhazardous. When cleaning up spills, remember to clean up any splashes that may have occurred on the sides of cabinets and doors in the immediate area.
- When working in cold rooms, keep all toxic and flammable substances tightly closed as cold rooms have recirculated atmospheres.
- Be aware of the potential asphyxiation hazard when using cryogenic materials and compressed gases in confined areas such as cold rooms and environmental chambers. If necessary, install an oxygen monitor/oxygen deficiency alarm and/or toxic gas monitor before working with these materials in confined areas. Contact EHS at 607-255-8200 for more assistance.
- Do not eat, drink, chew gum, or apply cosmetics in areas where hazardous chemicals are being used.
- Keep all food and drink out of refrigerators and freezers used to store chemicals. Refrigerators used to store chemicals should be labeled as “Chemicals Only – No Food”. Refrigerators used to store food should be labeled as “Food Only – No Chemicals”. You can download these and other free labels at the EHS Signs and Labels webpage.
- Always wash hands with soap and water after handling chemicals and especially before leaving the lab and eating – even if gloves were worn during chemical handling.
- Always remove personal protective equipment, such as gloves and lab coats, before leaving the lab.
- Do not attempt to scale up experiments until after you have run the experiment according to published protocols and you are thoroughly familiar with the potential hazards. When scaling up an experiment – change only one variable at a time, i.e. don’t change the heat source, the volumes, and the glassware all at once. It is also advisable to let one of your other lab group members to check your setup prior to each run.
7.2 Understanding Chemical Hazards7.2 Understanding Chemical Hazards
Chemicals pose both health and physical hazards. For the purposes of this document, health hazard will be used interchangeably with chemical hazard and health effects on the body will be used interchangeably with chemical effects on the body.
According to OSHA, health hazard means “a chemical for which there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed employees. The term ‘health hazard’ includes chemicals which are carcinogens, toxic or highly toxic agents, reproductive toxins, irritants, corrosives, sensitizers, hepatotoxins, nephrotoxins, neurotoxins, agents which act on the hematopoietic system and agents which damage the lungs, skin, eyes, or mucous membranes.”
According to OSHA, physical hazard means “a chemical for which there is scientifically valid evidence that it is a combustible liquid, a compressed gas, explosive, flammable, an organic peroxide, an oxidizer, pyrophoric, unstable (reactive) or water-reactive.” Physical hazards are covered in other sections within this manual.
7.2.1 Chemical Hazard Information7.2.1 Chemical Hazard Information
As part of the employers Chemical Hygiene Plan, the OSHA Laboratory Standard requires that “the employer shall provide employees with information and training to ensure that they are apprised of the hazards of chemicals present in their work area…Such information shall be provided at the time of an employee’s initial assignment to a work area where hazardous chemicals are present and prior to assignments involving new exposure situations.”
- This Laboratory Safety Manual
- Known reference materials (EHS maintains a reference library)
- Training videos (EHS maintains a video library available for loan)
- Other department’s safety manuals
- Safety Data Sheets (SDSs)
- EHS Training Programs
- Departmental Safety Committees
- Container labels
- Laboratory Standard Operating Procedures
- Laboratory signage and postings
- Publications such as the American Chemical Society – Safety in Academic Chemistry Laboratories
7.3 Safety Data Sheets7.3 Safety Data Sheets
Safety Data Sheets (SDSs) are an important part of any laboratory safety program in communicating information to chemical users. SDSs provide useful information such as:
- The identity of the chemical substance.
- Physical and chemical characteristics.
- Physical and health hazards.
- Primary routes of entry.
- OSHA Permissible Exposure Limits (PELs).
- Carcinogenic and reproductive health status.
- Precautions for safe handling and use (including PPE).
- Spill response procedures.
- Emergency and first aid questions.
- Date the SDS was prepared.
- Any chemical shipment received should be accompanied by an SDS (unless one has been shipped with a previous order). If you do not receive an SDS with your shipment, check the chemical manufacturers website first (or call the manufacturer directly), or check the EHS SDS website for links to SDSs, or contact EHS at 607-255-8200 to request assistance in obtaining the SDS.
If you have questions on how to read SDSs, or questions about the terminology or data used in SDSs, you can contact EHS at 607-255-8200 for more information. Additional information, including how to read an SDS, can be found in the SDS FAQ and a glossary of terms used on SDSs can be found in the “Hyperglossary”. Information on the National Fire Protection Association - NFPA diamond and the Hazardous Materials Information Guide and Hazardous Materials Information System – HMIG and HMIS - is also available.
SDSs must be accessible at all times. Access to SDSs can mean access to paper copies or electronic access via the internet. EHS maintains links to a number of SDS websites and other sites with chemical health and safety information.
EHS strongly encourages paper copies of SDSs be kept in the laboratory, however, having SDS websites bookmarked is acceptable as long as all employees in the workplace know where to find the SDSs and are trained on the use of computers to access SDSs. If a laboratory chooses to use electronic access, then EHS recommends the SDS website link be posted on the computer or in another conspicuous location. Some departments maintain three ring binders - “Big Red Books” - with SDSs. Check with your DSR for the location of the departmental SDS collection.
7.3.1 SDSs and Newly Synthesized Chemicals7.3.1 SDSs and Newly Synthesized Chemicals
Principal Investigators will be responsible for ensuring that newly synthesized chemicals are used exclusively within their laboratories and are properly labeled. If the hazards of a chemical synthesized in the laboratory are unknown, then the chemical must be assumed to be hazardous and the label should indicate the potential hazards of that substance have not been tested and are unknown.
The Principal Investigator must ensure a SDS is prepared for newly synthesized chemicals if:
- The chemical is hazardous according to the OSHA definition of hazardous (if the hazards are not known, then the chemical must be assumed to be hazardous).
- The newly created chemical or intermediate compound is going to be transferred to a different researcher or testing lab on or off of the Cornell University campus.
- The newly created chemical or intermediate compound is going to be kept in the lab for an on-going basis for use by current and/or future researchers in the lab where it was originally made.
- The newly created chemical or intermediate compound is going to be provided to another research group at Cornell University.
Additional information on developing SDSs can be found in the SDS FAQ. A blank SDS form (OSHA Form 174) can be found on the OSHA website. A description of how to fill out an SDS can be found in the appendix.
7.4 Routes of Chemical Entry7.4 Routes of Chemical Entry
The potential health effects that may result from exposure to chemicals depends on a number of factors. These factors include the properties of the specific chemical (including toxicity), the dose and concentration of the chemical, the route of exposure, duration of exposure, individual susceptibility, and any other effects resulting from mixtures with other chemicals.
In order to understand how chemical hazards can affect you, it is important to first understand how chemicals can get into your body and do damage. The four main routes of entry are inhalation, ingestion, injection, and absorption through the skin and eyes.
7.4.1 Inhalation7.4.1 Inhalation
Inhalation of chemicals occurs by absorption of chemicals via the respiratory tract (lungs). Once chemicals have entered into the respiratory tract, the chemicals can then be absorbed into the bloodstream for distribution throughout the body. Chemicals can be inhaled in the form of vapors, fumes, mists, aerosols and fine dust.
Symptoms of exposure to chemicals through inhalation include eye, nose, and throat irritation, coughing, difficulty in breathing, headache, dizziness, confusion, and collapse. If any of these symptoms are noted, leave the area immediately and get fresh air. Seek medical attention if symptoms persist and complete and Injury/Illness Report.
Laboratory workers can protect themselves from chemical exposure via inhalation through proper use of a functioning fume hood, use of dust masks and respirators when a fume hood is not available, avoiding bench top use of hazardous chemicals, ensuring chemical containers are kept tightly capped, and ensuring all chemical spills are promptly cleaned up.
7.4.2 Ingestion7.4.2 Ingestion
Chemical exposure through ingestion occurs by absorption of chemicals through the digestive tract. Ingestion of chemicals can occur directly and indirectly. Direct ingestion can occur by accidently eating or drinking a chemical; with proper housekeeping and labeling, this is less likely to occur. A higher probability of receiving a chemical exposure can occur by way of indirect ingestion. This can occur when food or drink is brought into a chemical laboratory. The food or drink can then absorb chemical contaminants (vapors or dusts) in the air and result in a chemical exposure when the food or drink is consumed. This can also occur when food or drink is stored with chemicals, such as in a refrigerator. Ingestion can occur when a laboratory worker who handles chemicals does not wear gloves or practice good personal hygiene, such as frequent hand washing, and then leaves the laboratory to eat, drink, or smoke. In all cases, a chemical exposure can result, although the effects of chronic exposure may not manifest itself until years later.
Symptoms of chemical exposure through ingestion include metallic or other strange tastes in the mouth, stomach discomfort, vomiting, problems swallowing, and a general ill feeling.
After seeking medical attention, complete an Injury/Illness Report.
The best protection against ingestion of chemicals is to properly label all chemical containers, never consume food or drink or chew gum in laboratories, always wear PPE (such as gloves), and practice good personal hygiene, such as frequent hand washing.
7.4.3 Injection7.4.3 Injection
Chemical exposure via injection can occur when handling chemically contaminated items such as broken glass, plastic, pipettes, needles, razor blades, or other items capable of causing punctures, cuts, or abrasions to the skin. When this occurs, chemicals can be injected directly into the bloodstream and cause damage to tissue and organs. Due to direct injection into the bloodstream, symptoms from chemical exposure may occur immediately.
Laboratory workers can protect themselves from an injection hazard by wearing proper PPE such as safety glasses/goggles, face shields, and gloves. Inspect all glassware for chips and cracks before use, and immediately discard any glassware or plasticware that is damaged. To help protect coworkers in the lab and building care staff, all broken glass should be disposed of in a puncture resistant container labeled as “Broken Glass”. This can be a commercially purchased “broken glass” container or simply a cardboard box or other puncture resistant container labeled as “Broken Glass”.
Whenever cleaning up broken glass or other sharp items, always use a broom, scoop or dustpan, or devices such as pliers, before using your hands to pick up broken pieces. If you have to use your hands, it is best to wear leather gloves when handling broken glass. For other items that can cause cuts or puncture wounds, such as needles and razor blades, never leave these items out in the open where someone could come into contact with them. EHS recommends using a device such as a piece of Styrofoam or similar item to secure them for later use. For disposal, use an appropriate “sharps” container.
If you do receive a cut or injection from a chemically contaminated item, if possible, gently try to remove the object and immediately rinse under water while trying to flush the wound and remove any chemical contamination, administer first aid and seek medical attention if necessary, and then complete an Injury/Illness Report.
7.4.4 Eye and Skin Absorption7.4.4 Eye and Skin Absorption
Some chemicals can be absorbed by the eyes and skin, resulting in a chemical exposure. Most situations of this type of exposure result from a chemical spill or splash to unprotected eyes or skin. Once absorbed by these organs, the chemical can quickly find its way into the bloodstream and cause further damage, in addition to the immediate effects that can occur to the eyes and the skin.
Symptoms of eye exposure can include itchy or burning sensations, blurred vision, discomfort, and blindness. The best way to protect yourself from chemical splashes to the eyes is to always wear safety glasses in the laboratory whenever eye hazards exist (chemicals, glassware, lasers, etc.). If you are pouring chemicals, then splash goggles are more appropriate than safety glasses. Whenever a severe splash hazard may exist, the use of a face shield, in combination with splash goggles is the best choice for protection.
If you do get chemicals in your eyes, immediately go to an eyewash station and flush your eyes for at least 15 minutes. The importance of flushing for at least 15 minutes cannot be overstated! Once the eyewash has been activated, use your fingers to hold your eyelids open and roll your eyeballs in the stream of water so the entire eye can be flushed. After flushing for at least 15 minutes, seek medical attention immediately and complete an Injury/Illness Report.
Symptoms of skin exposure to chemicals include dry, whitened skin, redness, swelling, rashes, blisters, itching, chemical burns, cuts, and defatting.
Laboratory workers can protect their skin from chemical exposure by selecting and wearing the proper gloves, wearing a lab coat and other personal protective equipment for special hazards (such as protective sleeves, face shields, and aprons), and not wearing shorts and sandals in areas where chemicals are being used - even if you are not using chemicals, but someone else in the lab is using chemicals nearby.
For small chemical splashes to the skin, remove any contaminated gloves, lab coats, etc., and wash the affected area with soap and water for at least 15 minutes. Seek medical attention afterward, especially if symptoms persist.
For large chemical splashes to the body, it is important to get to an emergency shower and start flushing for at least 15 minutes. Once under the shower, and after the shower has been activated, it is equally important to remove any contaminated clothing. Failure to remove contaminated clothing can result in the chemical being held against the skin and causing further chemical exposure and damage. After flushing for a minimum of 15 minutes, seek medical attention immediately and complete an Injury/Illness Report.
7.5 Chemical Exposure Limits7.5 Chemical Exposure Limits
The Permissible Exposure Limits (PEL) are based on the average concentration of a chemical to which workers can be exposed to over an 8-hour workday, 5 days per week, for a lifetime without receiving damaging effects. In some cases, chemicals can also have a Ceiling (C) limit, which is the maximum concentration that cannot be exceeded. OSHA has established PELs for over 500 chemicals. Permissible Exposure Limits are legally enforceable.
Another measure of exposure limits are Threshold Limit Values (TLV) which are recommended occupational exposure limits published by the American Conference of Governmental Industrial Hygienists (ACGIH). Similar to PELs, TLVs are the average concentration of a chemical that a worker can be exposed to over an 8-hour workday, 5 days per week, over a lifetime without observing ill effects. TLVs also have Ceiling (C) limits, which are the maximum concentration a worker can be exposed to at any given time. The ACGIH has established TLVs for over 800 chemicals. A main point of difference between PELs and TLVs is that TLVs are advisory guidelines only and are not legally enforceable. Both PELs and TLVs can be found in SDSs. Another good resource for information is the National Institute for Occupational Health and Safety (NIOSH).
7.6 Chemical Exposure Monitoring7.6 Chemical Exposure Monitoring
As a laboratory worker, you may use a variety of potentially hazardous materials on a daily basis. Safe use of these materials depends heavily on following proper laboratory work practices and the utilization of engineering controls. In certain circumstances, it is necessary to verify that work practices and engineering controls are effective in limiting exposures to hazardous materials. EHS Industrial Hygienists can help evaluate the effectiveness of your controls by monitoring exposures to a variety of laboratory materials. Exposure monitoring is the determination of the airborne concentration of a hazardous material in the work environment. Exposure monitoring data is compared to existing OSHA and ACGIH exposure guidelines and is often used to make recommendations concerning engineering controls, work practices, and PPE.
If you think you are receiving a chemical exposure in excess of OSHA exposure limits, such as feeling symptoms commonly associated with exposure to hazardous materials, or work with any of the chemicals listed below, contact EHS at 607-255-8200 and our Industrial Hygienists can use a variety of sampling methods to monitor for any potential exposures.
In some cases, OSHA substance specific standards actually require that the employer conduct initial exposure monitoring.
Examples of chemicals that fall into this category include:
- Vinyl chloride
- Methylene chloride
- Ethylene oxide
Other substances that have exposure monitoring requirements include:
7.7 Toxicity7.7 Toxicity
Toxicity refers to the ability of a chemical to cause harmful effects to the body. As was described by Paracelsus (1493-1541):
There are a number of factors that influence the toxic effects of chemicals on the body. These include, but are not limited to:
- The quantity and concentration of the chemical.
- The length of time and the frequency of the exposure.
- The route of the exposure.
- If mixtures of chemicals are involved.
7.7.1 Toxic Effects7.7.1 Toxic Effects
Toxic effects are generally classified as acute toxicity or chronic toxicity.
- Acute toxicity is generally thought of as a single, short-term exposure where effects appear immediately and are often reversible. An example of acute toxicity relates to the over consumption of alcohol and “hangovers”.
- Chronic toxicity is generally thought of as frequent exposures where effects may be delayed (even for years) and are generally irreversible. Chronic toxicity can also result in acute exposures, with long term chronic effects. An example of chronic toxicity relates to cigarette smoking and lung cancer.
7.7.2 Evaluating Toxicity Data7.7.2 Evaluating Toxicity Data
SDSs and other chemical resources generally refer to the toxicity of a chemical numerically using the term Lethal Dose 50 (LD50). The LD50 describes the amount of chemical ingested or absorbed by the skin in test animals that causes death in 50% of test animals used during a toxicity test study. Another common term is Lethal Concentration 50 (LC50), which describes the amount of chemical inhaled by test animals that causes death in 50% of test animals used during a toxicity test study. The LD50 and LC50 values are then used to infer what dose is required to show a toxic effect on humans.
As a general rule of thumb, the lower the LD50 or LC50 number, the more toxic the chemical. Note there are other factors (concentration of the chemical, frequency of exposure, etc.) that contribute to the toxicity of a chemical, including other hazards the chemical may possess.
While exact toxic effects of a chemical on test animals cannot necessarily be directly correlated with toxic effects on humans, the LD50 and LC50 can give a good indication of the toxicity of a chemical, particularly in comparison to another chemical. For example, when making a decision on what chemical to use in an experiment based on safety for the lab worker, a chemical with a high LD50 or LC50 would be safer to work with, assuming the chemical did not possess multiple hazards and everything else being equal.
In general terms, the resource Prudent Practices in the Laboratory lists the following table for evaluating the relevant toxicity of a chemical:
|Toxicity Class||Animal LD50||Probable Lethal Dose for 70 kg Person (150 lbs.)||Example|
|Super Toxic||Less than 5 mg/kg||A taste (7 drops or less)||Botulinum toxin|
|Extremely Toxic||5 - 50 mg/kg||< 1 teaspoonful||Arsenic trioxide, Strychnine|
|Very Toxic||50 - 500 mg/kg||< 1 ounce||Phenol, Caffeine|
|Moderately Toxic||0.5 - 5 g/kg||< 1 pint||Aspirin, Sodium chloride|
|Slightly Toxic||5 - 15 g/kg||< 1 quart||Ethyl alcohol, Acetone|
In addition to having a toxic effect on the body, some chemicals can be carcinogenic, mutagenic, teratogenic, and acutely toxic. These specific chemical hazards are covered in more detail under the Particularly Hazardous Substances section in this manual.
7.8 Chemical Labeling7.8 Chemical Labeling
The simple rule for chemical labeling is - if a container looks like it contains a chemical (even a clear liquid), then it must be labeled with the contents. Proper labeling of chemicals is one way of informing people who work in laboratories of potential hazards that exist, preventing the generation of unknowns, and facilitating emergency responses such as cleaning up spills and obtaining the proper medical treatment.
New chemical containers have the proper labeling information on the chemical label. The OSHA Laboratory Standard requires that labels on all incoming containers must be maintained and not defaced. As part of laboratory good housekeeping and self-inspections, if any chemical labels appear to be falling off, then laboratory personnel should tape the label back on the container or relabel with a permanent label.
7.8.1 Non-Original Containers7.8.1 Non-Original Containers
Non-original containers (secondary use containers) such as wash bottles, squirt bottles, temporary storage containers, beakers, flasks, bottles, vials, etc. or any container that a chemical from an original container is transferred into, must be properly labeled. In general, EHS recommends writing out the full chemical name and any hazards associated with that chemical. Laboratory personnel are strongly encouraged to use commercially available pre-labeled containers (such as squirt bottles) for chemicals that get used frequently. However, labs can also choose to label chemical containers in other ways such as:
1. Abbreviations - Structures and Formulas
Use of abbreviations such as structures, formulas, or acronyms is acceptable. However, if you use any abbreviations, you must hang up a “key” to the abbreviations in a visible location (preferably close to the chemicals and/or by the door). The “key” must contain the abbreviation and the name of the chemical. Including the hazards of the chemical on the “key” is also useful information. A sample fill-in the blank key can be found on the EHS Signs and Labels webpage. The abbreviation key must be readily available upon request by visitors, emergency responders, and state and federal regulatory agencies such as EPA, OSHA, or New York State Office of Fire Prevention and Control (OFPC) inspectors.
2. Small Containers and Sample Storage:
For small containers, such as vials and eppendorf tubes, which may be too small to write out a chemical name, structure, or formula, laboratories can implement other systems to identify the chemicals such as:
- Placing the vial or small container in a Ziploc bag or other type of overpack container (beaker, plastic bottle, etc.) and labeling the overpack container with the chemical name.
- Laboratories can use “price tag” style labels in which the chemical name is written out on a tag, and the tag is then attached to the small container with string or a rubber band.
- For vials in a test tube rack – laboratory personnel can simply label the rack with the chemical name, and then label the vials with an abbreviation, color, number, or letter code that corresponds to the label on the test tube rack. For example, if a lab had 10 small vials of ethanol in one rack, the rack could be labeled a 1-E = Ethanol. All of the vials would then be labeled as 1-E. Be sure that the number or letter code is clearly identifiable and would not be confused with other chemicals in the lab.
- For preserved specimens, bottles should be labeled with the preservative (i.e. ethanol or formaldehyde). A large number of these labels could easily be produced on the computer using Avery style mailing labels.
- For sample storage in refrigerators, laboratory personnel should label sample containers with one of the above methods, including labeling boxes that hold the small vials or chemical containers. Laboratories should include a key to any abbreviations on the outside of the refrigerator and label the key as “Sample Storage abbreviation = chemical name”.
3. Number, Letter, and Color Codes:
For vials and other small containers, laboratory personnel can make use of number, letter, and color-coded systems as long as a “key” is hung up which clearly identifies the chemical name that the number, letter, or color code represents. While this type of system is available for laboratory personnel to use, EHS does not recommend using such a system for hazardous chemicals. Such a system would be more appropriate for non-hazardous compounds such as agar and buffer solutions.
7.8.2 Labeling Requirements7.8.2 Labeling Requirements
In all cases, regardless of the labeling system used, the following labeling requirements must be followed:
- All chemical containers (both hazardous and non-hazardous) MUST be labeled. Chemical names must be written out in English. If a label is starting to fall off a chemical container or is becoming degraded, then the container needs to be relabeled (using tape, permanent marker, OSHA secondary labels, etc.) or the chemical needs to be transferred to another properly labeled container.
- If abbreviations such as formulas, structures, or acronyms are used, then a “key” to the abbreviations must be hung up in a conspicuous location.
- All personnel working in the laboratory must be fully trained on how to label chemicals using the system and how to understand the labeling system. Training must occur when a new person begins working in the laboratory, when new chemicals are introduced, and should occur on a regular basis or annually.
7.9 Chemical Storage7.9 Chemical Storage
Chemical storage areas in the academic laboratory setting include central stockrooms, storerooms, laboratory work areas, storage cabinets, refrigerators, and freezers. There are established legal requirements as well as recommended practices for proper storage of chemicals. Proper storage of chemicals promotes safer and healthier working conditions, extends the usefulness of chemicals, and can help prevent contamination. Chemicals that are stored improperly can result in:
- Degraded containers that can release hazardous vapors that are detrimental to the health of laboratory personnel.
- Degraded containers that allow chemicals to become contaminated, which can have an adverse effect on experiments.
- Degraded containers that can release vapors, which in turn can affect the integrity of nearby containers.
- Degraded labels that can result in the generation of unknowns.
- Chemicals becoming unstable and/or potentially explosive.
- Citation and/or fines from state and federal regulatory agencies.
7.9.1 General Storage Guidelines7.9.1 General Storage Guidelines
Laboratories should adhere to the following storage guidelines for the proper and safe storage of chemicals. By implementing these guidelines, laboratories can ensure safer storage of chemicals and enhance the general housekeeping and organization of the lab. Proper storage of chemicals also helps utilize limited laboratory space in a more efficient manner.
- All chemical containers MUST be labeled. Labels should include the name of the chemical constituent(s) and any hazards present. Be sure to check chemical containers regularly and replace any labels that are deteriorating or falling off and/or relabel with another label before the chemical becomes an unknown.
- Keep all containers of chemicals closed when not in use.
- Every chemical should have an identifiable storage place and should be returned to that location after use.
- The storage of chemicals on bench tops should be kept to a minimum to help prevent clutter and spills, and to allow for adequate working space.
- Chemical storage in fume hoods should be kept to a minimum - limited to the experiment being conducted. Excess storage of chemical containers in hoods can interfere with airflow, reduce working space, and increase the risk of a spill, fire, or explosion.
- For chemical storage cabinets, larger chemical bottles should be stored towards the back and smaller bottles should be stored up front where they are visible. Chemical bottles should be turned with the labels facing out so they can be easily read.
- Chemicals should not be stored on the floor due to the potential for bottles to be knocked over and result in a spill. If it is necessary to store bottles on the floor, then the bottles should be placed in secondary containment, such as trays, and the bottles should be placed away from aisle spaces.
- For multiples of the same chemical, older containers should be stored in front of newer chemicals and containers with the least amount of chemical should be stored in front of full containers. This allows for older chemicals to get used up first and helps to minimize the number of chemical containers in the storage area.
- Do not store chemicals in direct sunlight or next to heat sources.
- Laboratories should strive to keep only the minimum quantity of chemicals necessary. When ordering new chemicals, laboratories should only order enough stock needed for the experiment or the quantity that will get used up within 1 or 2 years at most.
- Liquid chemical containers should be stored in secondary containment, such as trays, to minimize the potential for bottle breakage and minimize the potential for spills.
- Always segregate and store chemicals according to compatibility and hazard classes.
- Chemical containers should be dated when they arrive and should be checked regularly and disposed of when they get past their expiration date. Please Note: Due to the potential explosion hazard, peroxide forming chemicals are required to be tested and dated.
- Flammable liquids in excess of quantities for specific flammability classes must be stored in approved flammable liquid storage cabinets.
- Do not store acids in flammable liquid storage cabinets. This can result in serious degradation of the storage cabinet and the containers inside. Corrosive chemicals should be stored in corrosion resistant cabinets. The exceptions to this rule are organic acids, such as Acetic acid, Lactic acid, and Formic acid, which are considered flammable/combustible and corrosive and can be stored in flammable or corrosive storage cabinets.
- Do not store corrosive or other chemicals that can be injurious to the eyes above eye level. In general and where practical, no chemicals should be stored above eye level.
- Label the outside of refrigerators/freezers to indicate items stored within. For example, "Chemicals only, no food".
- Do not store flammable liquids in standard (non-explosion proof) refrigerators or freezers. Due to the potential explosion hazard, only store flammables in refrigerators or freezers approved by the manufacturer for storage of flammables.
- Highly toxic chemicals such as inorganic cyanides should be stored in locked storage cabinets. Always keep the quantities of highly toxic chemicals to an absolute minimum. See Particularly Hazardous Substances section.
- Be aware of any special antidotes or medical treatment that may be required for some chemicals (such as Hydrofluoric acid).
- Always keep spill kits and other spill control equipment on hand in areas where chemicals are used. Ensure all personnel working in the lab have been properly trained on the location and use of the spill kit.
- For reagent shelves, it is recommended to use shelves with anti-roll lips, to prevent bottles from falling off. This can also be accomplished using heavy gauge twine or wire to create a lip on the shelf.
7.10 Transporting Chemicals7.10 Transporting Chemicals
When transporting chemicals between laboratories or other buildings on campus, the following guidelines should be implemented for protection of people and the environment, and to minimize the potential for spills to occur.
- Whenever transporting chemicals by hand, always use a secondary container such as a rubber acid carrying bucket, plastic bucket, or a 5-gallon pail). If necessary, a small amount of packing material (shipping peanuts, vermiculite, or cardboard inserts), that is compatible with the chemical(s), should be used to prevent bottles from tipping over or breaking during transport. You should have proper PPE accessible in the event of a spill.
- Wheeled carts with lipped surfaces (such as Rubbermaid carts) should be used whenever feasible.
- Whenever possible, do not use passenger elevators when transporting chemicals, only freight elevators should be used. If it is necessary to use a passenger elevator, use should be restricted to low-use times such as early in the morning or late in the afternoon. If this is not possible, be sure to warn passengers, or prohibit passengers from riding with you.
- When transporting compressed gas cylinders, always use a proper gas cylinder hand truck with the cylinder strapped to the cart and keep the cap in place. NEVER roll or drag a compressed gas cylinder.
- Avoid riding in elevators with cryogenic liquids or compressed gas cylinders. If this is necessary, consider using a buddy system to have one person send the properly secured dewars or cylinders on the elevator, while the other person waits at the floor by the elevator doors where the dewars or cylinders will arrive.
- Do not transport chemicals in your personal vehicle. Contact EHS at 607-255-8200 for assistance.
7.11 Chemical Segregation7.11 Chemical Segregation
Chemicals should be stored according to compatibility and hazard classes. Rather than store chemicals alphabetically, or by carbon number, or by physical state, etc., EHS recommends that you segregate them by DOT hazard class first. The potential hazards of storing incompatible chemicals together, and when an emergency occurs, include:
- Generation of heat.
- Possible fires and explosion.
- Generation of toxic and/or flammable gases and vapors.
- Formation of toxic compounds.
- Formation of shock and/or friction sensitive compounds.
- Violent polymerization.
- The benefits of chemical segregation by hazard class include:
- Safer chemical storage.
- Understanding the hazards a chemical exhibits will increase your knowledge about the chemical.
- Identifying potentially explosive chemicals.
- Identifying multiple containers of the same chemical.
There are a number of segregation schemes recommended in the literature by government agencies, chemical manufacturers, safety supply companies, and other universities. However, EHS is recommending segregation of chemicals using a modified version of the Department of Transportation (DOT) Hazard Class System. While this modified DOT system results in most common chemicals being segregated properly, there is no one system that solves all problems. The modified DOT system is less complicated than other segregation schemes and the information to make decisions of which hazard classes to use can easily be found in SDSs, container labels, container markings and stickers, and other resources.
When you are making decisions on how to segregate, keep in mind the following:
- Physical hazards of the chemical.
- Health hazards of the chemical.
- The chemical form (solid, liquid or gas).
- Concentration of the chemical.
Segregation of different chemical hazard classes (such as acids and bases) can occur in the same cabinet as long as there is some form of physical separation, such as using trays with high sides or deep trays. However never store oxidizers and flammables in the same cabinet. Also, do not store compounds such as inorganic cyanides and acids in the same cabinet.
Once chemicals have been segregated, ensure everyone in the lab knows the process and what system is being used. It is best to clearly identify where chemicals in each hazard class will be stored by labeling cabinets with signs, or hazard class labels. These can be purchased from a safety supply company, you can create your own, or download FREE labels from the EHS Signs and Labels webpage.
If you need assistance with cleaning out your lab of old and excess chemicals, or would like assistance with segregating your chemicals, contact EHS at askEHS@cornell.edu. EHS also offers an online training class on Chemical Segregation. Examples of incompatible chemicals can be found in the appendix.
7.11.1 EHS Modified DOT Hazard Class System7.11.1 EHS Modified DOT Hazard Class System
The basic DOT hazard classes and hazard class numbers are:
|DOT Hazard Class Number||Hazard Class|
|Class 2||Compressed gases|
|Class 3||Flammable liquids|
|Class 4||Flammable solids|
|Class 7||Radioactive materials|
|Class 9||Store with Class 6|
The DOT hazard class numbers can be found on hazard class labels, in SDSs (under the “Transportation Information Section”), on container labels, and in other reference texts. An explanation of the DOT Hazard Class system can be found in the DOT Training Modules and an expanded version of the DOT hazard classes can be found on the EHS Signs and Labels webpage.
The EHS chemical segregation scheme modifies the DOT system by breaking down hazard classes into subcategories. A handout on the EHS Chemical Segregation Scheme can be found in the appendix.
Chapter 8 - Chemical HazardsChapter 8 - Chemical Hazards
Chemicals can be broken down into hazard classes and exhibit both physical and health hazards. It is important to keep in mind, that chemicals can exhibit more than one hazard or combinations of several hazards. Several factors can influence how a chemical will behave and the hazards the chemical presents, including the severity of the response:
- Concentration of the chemical.
- Physical state of the chemical (solid, liquid, gas).
- Physical processes involved in using the chemical (cutting, grinding, heating, cooling, etc.).
- Chemical processes involved in using the chemical (mixing with other chemicals, purification, distillation, etc.).
- Other processes (improper storage, addition of moisture, storage in sunlight, refrigeration, etc.).
The following sections describe general information and safety precautions about specific hazard classes. The chemical hazards listed are based on the Department of Transportation (DOT) hazard class system (which will be discussed in the Chemical Segregation section and where appropriate, will be noted as such). A listing of the DOT hazard classes can be found on the EHS Signs and Labels webpage. A general description of the hazards of various chemical functional groups can be found in the appendix.
- OSHA Lab Standard
- Department of Transportation (DOT) hazard class system
- Safety in Academic Chemistry Laboratories
- Safety Data Sheets Webpage
- Cornell Health
- OSHA Definition of Health Hazard
- EHS Signs and Labels webpage
- Prudent Practices in the Laboratory
- EHS Online Training Programs
- Cornell Health
8.1 Explosives8.1 Explosives
The OSHA Laboratory Standard defines an explosive as a chemical that causes a sudden, almost instantaneous release of pressure, gas, and heat when subjected to sudden shock, pressure, or high temperature. Under the Department of Transportation (DOT) hazard class system, explosives are listed as hazard class 1.
Fortunately, most laboratories do not use many explosives; however, there are a number of chemicals that can become unstable and/or potentially explosive over time due to contamination with air, water, other materials such as metals, or when the chemical dries out.
Explosives can result in damage to surrounding materials (hoods, glassware, windows, people, etc.), generation of toxic gases, and fires. If you plan to conduct an experiment where the potential for an explosion exists, first ask yourself the question; “Is there another chemical that could be substituted in the experiment that does not have an explosion potential?” If you must use a chemical that is potentially explosive, or for those compounds that you know are explosive, (even low powered explosives) you must first obtain prior approval from the Principal Investigator to use such chemicals. After obtaining prior approval from your Principal Investigator, thoroughly read the SDSs and any other chemical resources related to the potentially explosive compound(s) to ensure potential incidents are minimized.
Whenever setting up experiments using potentially explosive compounds:
- Always use the smallest quantity of the chemical possible.
- Always conduct the experiment within a fume hood and use in conjunction with a properly rated safety shield.
- Be sure to remove any unnecessary equipment and other chemicals (particularly highly toxic and flammables) away from the immediate work area.
- Be sure to notify other people in the laboratory what experiment is being conducted, what the potential hazards are, and when the experiment will be run.
- Do not use metal or wooden devices when stirring, cutting, scraping, etc. with potentially explosive compounds. Non-sparking plastic devices should be used instead.
- Ensure other safety devices such as high temperature controls, water overflow devices, etc., are used in combination to help minimize any potential incidents.
- Properly dispose of any hazardous waste and note on the hazardous waste tag any special precautions that may need to be taken if the chemical is potentially explosive.
- Always wear appropriate PPE, including the correct gloves, lab coat or apron, safety goggles used in conjunction with a face shield, and explosion-proof shields when working with potentially explosive chemicals.
For storage purposes, always date chemical containers when received and opened. Pay particular attention to those compounds that must remain moist or wet so they do not become explosive (ex. Picric acid, 2,4-Dinitrophenyl hydrazine, etc.). Pay particular attention to any potentially explosive compounds that appear to exhibit the following signs of contamination:
- Deterioration of the outside of the container.
- Crystalline growth in or outside the container.
- Discoloration of the chemical.
If you discover a potentially explosive compound that exhibits any of these signs of contamination, contact EHS at 607-255-8200 for more assistance.
Examples of explosive and potentially explosive chemicals include:
- Compounds containing the functional groups azide, acetylide, diazo, nitroso, haloamine, peroxide, and ozonide
- Di- and Tri-nitro compounds
- Peroxide forming compounds
- Picric acid (dry)
- 2,4-Dinitrophenylhydrazine (dry)
- Benzoyl peroxide (dry)
8.2 Flammable and Combustible Liquids8.2 Flammable and Combustible Liquids
The OSHA Laboratory Standard defines a flammable liquid as any liquid having a flashpoint below 100 degrees F (37.8 degrees C), except any mixture having components with flashpoints of 100 degrees F (37.8 degrees C) or higher, the total of which make up 99% or more of the total volume of the mixture.
Flashpoint is defined as the minimum temperature at which a liquid gives off enough vapor to ignite in the presence of an ignition source. The risk of a fire requires that the temperature be above the flashpoint and the airborne concentration be in the flammable range above the Lower Explosive Limit (LEL) and below the Upper Explosive Limit (UEL).
The OSHA Laboratory Standard defines a combustible liquid as any liquid having a flashpoint at or above 100 degrees F (37.8 degrees C), but below 200 degrees F (93.3 degrees C), except any mixture having components with flashpoints of 200 degrees F (93.3 degrees C), or higher, the total volume of which make up 99% or more of the total volume of the mixture. OSHA further breaks down flammables into Class I liquids, and combustibles into Class II and Class III liquids.
|Liquid Type||Classification||Flash Point||Boiling Point|
|Flammable Liquid||Class IA||<73 degrees F||<100 degrees F|
|Class IB||<73 degrees F||>=100 degrees F|
|Class IC||>=73 degrees F, <100 degrees F||>100 degrees F|
|Combustible Liquid||Class II||>=100 degrees F, <140 degrees F||no data|
|Class IIIA||>=140 degrees F, < 200 degrees F||no data|
|Class IIIB||>=200 degrees F||no data|
Under the Department of Transportation (DOT) hazard class system, flammable liquids are listed as hazard class 3.
Flammable and combustible liquids are one of the most common types of chemicals used at Cornell and are an important component in a number of laboratory processes. However, in addition to the flammable hazard, some flammable liquids also may possess other hazards such as being toxic and/or corrosive.
When using flammable liquids, keep containers away from open flames; it is best to use heating sources such as steam baths, water baths, oil baths, and heating mantels. Never use a heat gun to heat a flammable liquid. Any areas using flammables should have a fire extinguisher present. If a fire extinguisher is not present, then contact EHS at 607-255-8200 for more assistance.
Always keep flammable liquids stored away from oxidizers and away from heat or ignition sources such as radiators, electric power panels, etc.
When pouring flammable liquids, it is possible to generate enough static electricity to cause the flammable liquid to ignite. If possible, make sure both containers are electrically interconnected to each other by bonding the containers, and connecting to a ground.
Always clean up any spills of flammable liquids promptly. Be aware that flammable vapors are usually heavier than air (vapor density > 1). For those chemicals with vapor densities heavier than air (applies to most chemicals), it is possible for the vapors to travel along floors and, if an ignition source is present, result in a flashback fire.
8.2.1 Flammable Storage in Refrigerators/Freezers8.2.1 Flammable Storage in Refrigerators/Freezers
It is important to store flammable liquids only in specially designed flammable storage refrigerators/freezers or explosion-proof refrigerators/freezers. Do not store flammable liquids in standard (non-flammable rated) refrigerators/freezers. Standard refrigerators are not electrically designed to store flammable liquids. If flammable liquids are stored in a standard refrigerator, the build up of flammable vapors can be in sufficient quantities to ignite when the refrigerator’s compressor or light turns on, resulting in a fire or an explosion.
Properly rated flammable liquid storage refrigerators/freezers have protected internal electrical components and are designed for the storage of flammable liquids. Explosion-proof refrigerators/freezers have both the internal and external electrical components properly protected and are designed for the storage of flammable liquids. Refrigerators and freezers rated for the storage of flammable materials will be clearly identified as such by the manufacturer.
For most laboratory applications, a flammable storage refrigerator/freezer is acceptable. However, some operations may require an explosion-proof refrigerator/freezer. Flammable storage refrigerators currently cost approximately $1500 - $3000 each. In the case of limited funding where a laboratory cannot purchase a flammable storage refrigerator for the laboratory’s own use, EHS strongly encourages departments and laboratory groups on each floor to consider purchasing a communal flammable storage refrigerator for the proper and safe storage of flammable liquids.
8.2.2 Flammable Storage Cabinets8.2.2 Flammable Storage Cabinets
The requirements for the use of flammable storage cabinets are determined by the classification of the flammable liquids, the quantities kept on hand, the building construction (fire wall ratings), and the floor of the building the flammables are being stored on.
For stand-alone flammable cabinets (as opposed to cabinets underneath fume hoods), there are vent holes on each side of the cabinet (called bung holes) that must have the metal bungs screwed into place for the cabinet to maintain its fire rating. Venting of flammable cabinets is NOT required, however, if a flammable cabinet is vented, the cabinet must be vented properly according to the manufacturer’s specifications and NFPA 30.
Typically, proper flammable cabinet ventilation requires that air be supplied to the cabinet and the air be taken away via non-combustible pipes. If you plan to vent your flammable storage cabinet, please contact EHS at 607-255-8200 or askEHS@cornell.edu for more information.
8.3 Flammable Solids8.3 Flammable Solids
The OSHA Laboratory Standard defines a flammable solid as a “solid, other than a blasting agent or explosive, that is liable to cause fire through friction, absorption of moisture, spontaneous chemical change, or retained heat from manufacturing or processing, or which can be ignited readily and when ignited, burn so vigorously and persistently to create a serious hazard.” An example of a flammable solid is gun powder.
Under the DOT hazard class system, flammable solids are listed as hazard class 4. Flammable solids are further broken down into three subcategories:
- Flammable Solids – Class 4.1
- Spontaneously Combustible – Class 4.2
- Dangerous When Wet – Class 4.3
- Many of the same principles for handling and storage of flammable liquids apply to flammable solids. Always keep flammable solids stored away from oxidizers, and away from heat or ignition sources such as radiators, electric power panels, etc.
8.4 Spontaneously Combustible8.4 Spontaneously Combustible
Spontaneously combustible materials are also known as pyrophorics; these chemicals can spontaneously ignite in the presence of air, some are reactive with water vapor, and most are reactive with oxygen. Two common examples are tert-Butyllithium under Hexanes and White Phosphorus. In addition to the hazard of the spontaneously combustible chemical itself, many of these chemicals are also stored under flammable liquids. In the event of an accident, such as a bottle being knocked off a shelf, the chemical can spontaneously ignite and a fire can occur. Extra care must be taken when handling spontaneously combustible chemicals. When transporting these chemicals, it is best to use a bottle carrier and carts.
8.5 Dangerous When Wet8.5 Dangerous When Wet
Dangerous when wet compounds react violently with water to form toxic vapors and/or flammable gases that can ignite and cause a fire.
It is important to note that any paper toweling, gloves, etc., that have come into contact with these materials need to be quenched with water before disposing of in metal trash cans in order to prevent potential fires.
If you are using dangerous when wet compounds and do not have a Class D fire extinguisher present, then please contact EHS at 607-255-8200 for more assistance.
8.6 Oxidizers and Organic Peroxides8.6 Oxidizers and Organic Peroxides
The OSHA Laboratory Standard defines an oxidizer as “a chemical other than a blasting agent or explosive that initiates or promotes combustion in other materials, thereby causing fire either of itself or through the release of oxygen or other gases.” Under the DOT hazard class system, oxidizers are listed as hazard class 5.1 and organic peroxides are listed as hazard class 5.2.
The OSHA Laboratory Standard defines an organic peroxide as “an organic compound that contains the bivalent –O-O- structure and which may be considered to be a structural derivative of hydrogen peroxide where one or both of the hydrogen atoms have been replaced by an organic radical.”
Oxidizers and organic peroxides are a concern for laboratory safety due to their ability to promote and enhance the potential for fires in labs.
As a reminder of the fire triangle (now referred to as the fire tetrahedron), in order to have a fire, you need:
- A fuel source.
- An oxygen source.
- An ignition source.
- A chemical reaction.
Oxidizers can supply the oxygen needed for the fire, whereas organic peroxides supply both the oxygen and the fuel source. Both oxidizers and organic peroxides may become shock sensitive when they dry out, are stored in sunlight, or due to contamination with other materials, particularly when contaminated with heavy metals. Most organic peroxides are also temperature sensitive.
As with any chemicals, but particularly with oxidizers and organic peroxides, quantities stored on hand should be kept to a minimum. Whenever planning an experiment, be sure to read the SDS and other reference documents to understand the hazards and special handling precautions that may be required, including use of a safety shield. Also be aware of the melting and autoignition temperatures for these compounds and ensure any device used to heat oxidizers has an overtemperature safety switch to prevent the compounds from overheating.
Laboratory staff should be particularly careful when handling oxidizers (especially high surface area oxidizers such as finely divided powders) around organic materials.
Avoid using metal objects when stirring or removing oxidizers or organic peroxides from chemical containers. Plastic or ceramic implements should be used instead. Laboratory personnel should avoid friction, grinding, and impact with solid oxidizers and organic peroxides. Glass stoppers and screw cap lids should always be avoided and plastic/polyethylene lined bottles and caps should be used instead.
If you suspect your oxidizer or organic peroxide has been contaminated (evident by discoloration of the chemical, or if there is crystalline growth in the container or around the cap), then dispose of the chemical as hazardous waste or contact EHS at 607-255-8200. Indicate on the hazardous waste tag that the chemical is an oxidizer or organic peroxide and that you suspect contamination.
8.7 Peroxide Forming Compounds8.7 Peroxide Forming Compounds
Many commonly used chemicals; organic solvents in particular, can form shock, heat, or friction sensitive peroxides upon exposure to oxygen. Once peroxides have formed, an explosion can result during routine handling, such as twisting the cap off a bottle – if peroxides are formed in the threads of the cap. Explosions are more likely when concentrating, evaporating, or distilling these compounds if they contain peroxides.
When these compounds are improperly handled and stored, a serious fire and explosion hazard exists. The following guidelines should be adhered to when using peroxide forming chemicals:
- Each peroxide forming chemical container MUST be dated when received and opened. A list of common peroxide forming chemicals can be found in the appendix. Those compounds in the appendix listed in Table A should be disposed of within 3 months of opening and those compounds in the appendix listed in Tables B, C, and D should be disposed of within 12 months of opening.
- Each peroxide forming chemical container must be tested for peroxides when opened and at least every 6 months thereafter. The results of the peroxide test and the test date must be marked on the outside of the container. There are sample peroxide labels on the Signs and Labels webpage.
- Peroxide test strips can be purchased from the Chemistry Department stockroom or from a variety of safety supply vendors, such as VWR and Laboratory Safety Supply. An alternative to peroxide test strips is the KI (potassium iodide) test. References such as Prudent Practices in the Laboratory and the American Chemical Society booklet Safety in Academic Chemistry Laboratories outline ways to test for peroxides and ways to remove them if discovered. When using the test strips, if the strip turns blue, then peroxides are present. Light blue test results may be acceptable for use if your procedure does not call for concentrating, evaporating or distilling. Containers with darker blue test results must be deactivated or disposed of. You can test older test strips for efficacy with a dilute solution of hydrogen peroxide.
- Due to sunlight’s ability to promote formation of peroxides, all peroxidizable compounds should be stored away from heat and sunlight.
- Peroxide forming chemicals should not be refrigerated at or below the temperature at which the peroxide forming compound freezes or precipitates as these forms of peroxides are especially sensitive to shock and heat. Refrigeration does not prevent peroxide formation.
- As with any hazardous chemical, but particularly with peroxide forming chemicals, the amount of chemical purchased and stored should be kept to an absolute minimum. Only order the amount of chemical needed for the immediate experiment.
- Ensure containers of peroxide forming chemicals are tightly sealed after each use and consider adding a blanket of an inert gas, such as Nitrogen, to the container to help slow peroxide formation.
- A number of peroxide forming chemicals can be purchased with inhibitors added. Unless absolutely necessary for the research, labs should never purchase uninhibited peroxide formers.
- Before distilling any peroxide forming chemicals, always test the chemical first with peroxide test strips to ensure there are no peroxides present. Never distill peroxide forming chemicals to dryness. Leave at least 10-20% still bottoms to help prevent possible explosions.
While no definitive amount of peroxide concentration is given in the literature, a concentration of 50 ppm should be considered dangerous and a concentration of >100 ppm should be disposed of immediately. In both cases, procedures should be followed for removing peroxides or the containers should be disposed of as hazardous waste.
For those compounds that must be handled by an outside environmental “bomb squad” company, the cost for such an operation can result in charges of >$1000 per container. However, if laboratory staff follow the guidelines listed above, the chances for requiring special handling for these types of containers or for an explosion to occur is greatly diminished.
The appendix contains a listing of common peroxide forming chemicals. Please note this list is not all-inclusive, there are numerous other chemicals that can form peroxides. Be sure to read chemical container labels, SDSs, and other chemical references.
8.8 Poisons8.8 Poisons
For the purpose of this manual the word “Poison” will be used interchangeably with the word “Toxic”. OSHA defines “Toxic” as a chemical falling within any of the following categories:
- A chemical that has a median lethal dose (LD50) of more than 50 milligrams per kilogram, but not more than 500 milligrams per kilogram of body weight when administered orally to albino rats weighing between 200 and 300 grams each.
- A chemical that has a median lethal dose (LD50) of more than 200 milligrams per kilogram, but not more than 1000 milligrams per kilogram of body weight when administered by continuous contact for 24 hours (or less if death occurs within 24 hours) with the bare skin of albino rabbits weighing between two and three kilograms each.
- A chemical that has a median lethal concentration (LC50) in air of more than 200 parts per million, but not more than 2000 parts per million by volume of gas or vapor, or more than two milligrams per liter but not more than 20 milligrams per liter of mist, fume, dust, when administered by continuous inhalation for one hour (or less if death occurs with in one hour) to albino rats weighing between 200 and 300 grams each.
OSHA draws a distinction between toxic chemicals and acutely toxic chemicals. For more information on acutely toxic chemicals, see Particularly Hazardous Substances. OSHA also provides definitions for other health hazards on their website. Under the DOT hazard class system, poisons are listed as hazard class 6.
When working with known poisons, it is very important to have thought an experiment through, addressing health and safety issues before working with the poison. Safety Data Sheets (SDS) and other chemical references should be consulted before beginning the experiment. Some questions to ask before working with poisonous chemicals:
- Do I need to use the poisonous chemical or can a less toxic chemical be substituted?
- What are the routes of entry into the body for the poison (inhalation, ingestion, injection, or skin absorption)?
- What are the signs and symptoms of potential chemical exposure?
- What are the proper PPE required (type of glove, safety glasses vs. splash goggles, face shield, etc.)?
- Does the chemical require any special antidote?
- What are the emergency procedures to be followed?
When working with highly toxic chemicals, you should not work alone. Always wear proper PPE and always wash your hands with soap and water when finished, even if gloves were worn. Be aware that poisonous mixtures, vapors, and gases can be formed during an experiment. Be sure to research both the reactants and products of the chemicals you will be working with first. Additional information can be found in the Exposure Monitoring section and Routes of Chemical Entry section.
8.9 Corrosives8.9 Corrosives
OSHA defines a corrosive as “a chemical that causes visible destruction of, or irreversible alterations in living tissue by chemical action at the site of contact.” Under the DOT hazard class system, corrosives are listed as hazard class 8.
Corrosive chemicals can be further subdivided as acids and bases. Corrosives can be in the liquid, solid, or gaseous state. Corrosive chemicals can have a severe effect on eyes, skin, respiratory tract, and gastrointestinal tract if an exposure occurs. Corrosive solids and their dusts can react with moisture on the skin or in the respiratory tract and result in an exposure.
Whenever working with concentrated corrosive solutions, splash goggles should be worn instead of safety glasses. Splash goggles used in conjunction with a face shield provides better protection.
Corrosive chemicals should be handled in a fume hood to avoid breathing corrosive vapors and gases.
When mixing concentrated acids with water, always add acid slowly to the water (specifically, add the more concentrated acid to the dilute acid). Never add water to acid, this can result in a boiling effect and cause acid to splatter. Do not pour the acid directly into the water; it should be poured in a manner that allows it to run down the sides of the container. Never store corrosive chemicals above eye level and always use a protective bottle carrier when transporting corrosive chemicals.
Some chemicals can react with acids and liberate toxic and/or flammable vapors. When working with corrosive materials, ensure spill cleanup material is available for neutralization, such as Calcium carbonate for acids and Citric acid for bases.
Wherever acids and bases are used, an eyewash and emergency shower must be available. If any corrosive chemical gets splashed in the eyes, immediately go to an eyewash station and flush your eyes for at least 15 minutes. The importance of flushing for at least 15 minutes cannot be overstated! Once the eyewash has been activated, use your fingers to hold your eyelids open and roll your eyeballs in the stream of water so the entire eye can be flushed. After flushing for at least 15 minutes, seek medical attention immediately and complete an Injury/Illness Report.
For small splashes of corrosives to the skin, remove any contaminated gloves, lab coats, etc., and wash the affected area with soap and water for at least 15 minutes. Seek medical attention afterward, especially if symptoms persist.For large splashes of corrosives to the body, it is important to get to an emergency shower and start flushing for at least 15 minutes. Once under the shower, and after the shower has been activated, it is equally important to remove any contaminated clothing. Failure to remove contaminated clothing can result in the chemical being held against the skin and causing further chemical exposure and damage. After flushing for a minimum of 15 minutes, seek medical attention immediately and complete an Injury/Illness Report.
- Hydrofluoric Acid Designated Area Sign (docx)
- Hydrofluoric Acid Prior Approval Form
- OSHA Definition of Health Hazard
- Prudent Practices in the Laboratory
- EHS Online Training Programs
- Hydrofluoric Acid Information from Honeywell
- Hydrofluoric Acid First Aid Sign (docx)
Please note: some chemicals, such as Hydrofluoric acid, require the use of a special antidote (such as Calcium gluconate gel) and special emergency procedures. Read the SDSs for any chemical(s) you work with to determine if a special antidote is needed if a chemical exposure occurs.
8.9.1 Hydrofluoric Acid8.9.1 Hydrofluoric Acid
Hydrofluoric Acid (HF) is one of the most hazardous chemicals at used Cornell. Small exposures to HF can be fatal if not treated properly. The critical minutes immediately after an exposure can have a great effect on the chances of a victim’s survival.
HF is a gas that is dissolved in water to form Hydrofluoric acid. The concentration can vary from very low such as in store bought products up to the most concentrated 70% form (anhydrous), with the most common lab use around 48%. The liquid is colorless, non-flammable and has a pungent odor. The OSHA permissible exposure limit is 3 ppm, but concentrations should be kept as low as possible. HF is actually a weak acid by definition and not as corrosive as strong acids such as Hydrochloric (HCl), however, corrosivity is the least hazardous aspect of HF. The toxicity of HF is the main concern.
HF is absorbed through the skin quickly and is a severe systemic toxin. The fluoride ion binds calcium in the blood, bones and other organs and causes damage to tissues that is very painful and can be lethal. At the emergency room, the victim is often given calcium injections, but pain medication is not generally given since the pain subsiding is the only indication that the calcium injections are working.
Due to the serious hazard of working with HF, the following requirements and guidelines are provided:
- All users of HF must receive EHS Hydrofluoric Acid Safety training as well as training by their supervisor. The EHS Hydrofluoric Acid Safety training is available online.
- A Standard Operating Procedure (SOP) must be written for the process in which HF is used. This SOP should be posted or readily available near the designated area where HF use will occur.
- HF should only be used in a designated fume hood and the fume hood should be identified by posting a HF Designated Area Sign (docx).
- First Aid - A HF first aid kit must be available that includes 2.5% calcium gluconate gel. The Calcium gluconate gel can be obtained at the Cornell Health dispensary with a department charge number and should be replaced with new stock annually. The Hydrofluoric Acid First Aid Sign (docx) should be posted in a prominent place where the Calcium gluconate gel is located.
- Spill Kits - An HF spill kit must be available with calcium compounds such as Calcium carbonate, Calcium sulfate or Calcium hydroxide. Sodium bicarbonate should never be used since it does not bind the fluoride ion and can generate toxic aerosols.
Prior approval - Before anyone uses HF they must have prior approval from the Principal investigator. The names of lab personnel should be added to an HF Prior Approval form showing that they have are familiar with the following:
- Has read the SDS for HF
- Has read the HF Use SOP developed by the lab
- Has read the Hydrofluoric acid section in this Lab Safety Manual
- Is aware of the designated area for HF use
- Knows the first aid procedure in case of an HF exposure
- Knows what to do incase of an HF spill
Personal Protective Equipment (PPE) – The following PPE is required for HF use:
- Rubber or plastic apron
- Plastic arm coverings
- Incidental use - double glove with heavy nitrile exam gloves and re-glove if any exposure to the gloves
- Extended use – heavy neoprene or butyl over nitrile or silver shield gloves
- Splash goggles in conjunction with a fume hood sash
- Closed toed shoes
- Long pants and a long sleeve shirt with a reasonably high neck (no low cut)
The following are safe practice guidelines when working with HF:
- Never work alone with HF but have a buddy system.
- Use a plastic tray while working with HF for containment in case of a spill.
- Keep containers of HF closed. HF can etch the glass sash and make it hard to see through (if the hood sash becomes fogged and hard to see though due to etching, then please contact EHS at 607-255-8200 about installing a polycarbonate sash)
- Safety Data Sheet (SDS) – A SDS for HF must be available.
- All containers of HF must be clearly labeled. Secondary labels for all non-original containers can be printed from Chemwatch.
- The stock HF should be stored in plastic secondary containment and the cabinet should be labeled. HF should be stored in lower cabinets near the floor.
- Wash gloves off with water before removing them.
Additional information on the safe use and handling of Hydrofluoric acid (HF) can be found on the Honeywell website - the world's largest producer of Hydrofluoric Acid. This website contains useful information on HF such as:
- Safety Data Sheets
- Technical Data Sheets
- Recommended Medical Treatment for HF exposure
- HF Properties charts
- Online Training
8.9.2 Perchloric Acid8.9.2 Perchloric Acid
Perchloric acid is a strong oxidizing acid that can react violently with organic materials. Perchloric acid can also explode if concentrated above 72%. For any work involving heated Perchloric acid (such as in Perchloric acid digestions), the work must be conducted in a special Perchloric acid fume hood with a wash down function. If heated Perchloric acid is used in a standard fume hood, the hot Perchloric acid vapors can react with the metal in the hood ductwork to form shock sensitive metallic perchlorates. When working with Perchloric acid, be sure to remove all organic materials, such as solvents, from the immediate work area. Due to the potential danger of Perchloric acid, if possible, try to use alternate techniques that do not involve the use of Perchloric acid. If you must use Perchloric acid in your experiments, only purchase the smallest size container necessary.
Because Perchloric acid is so reactive, it is important to keep it stored separate from other chemicals, particularly organic solvents, organic acids, and reducing agents. All containers of Perchloric acid should be inspected regularly for container integrity and the acid should be checked for discoloration. Discolored Perchloric acid should be discarded as hazardous waste. Perchloric acid should be used and stored away from combustible materials, and away from wooden furniture. Like all acids, but particularly with Perchloric acid, secondary containment should be used for storage.
Chapter 9 - Particularly Hazardous SubstancesChapter 9 - Particularly Hazardous Substances
The OSHA Laboratory Standard requires as part of the Chemical Hygiene Plan that provisions for additional employee protection be included for work involving particularly hazardous substances. These substances include “select carcinogens”, reproductive toxins, and substances which have a high degree of acute toxicity. Each of these categories will be discussed in detail in later sections.The OSHA Laboratory Standard states for work involving particularly hazardous substances, specific consideration be given to the following provisions where appropriate:
- Establishment of a designated area.
- Use of containment devices such as fume hoods or glove boxes.
- Procedures for safe removal of contaminated waste.
- Decontamination procedures.
EHS can assist researchers by providing information on working with particularly hazardous substances. General guidelines and recommendations for the safe handling, use, and control of hazardous chemicals and particularly hazardous substances can be found in SDSs.
- OSHA Lab Standard
- OSHA Reproductive Hazards Topics Webpage
- National Toxicology Program
- Carcinogens Known to the State of California through Prop 65
- Institutional Animal Care and Use Committee
- Radiation Safety Group
- Poison Inhalation Hazard Purchasing Policy
- Cornell Health
- Prudent Practices in the Laboratory
- EHS Online Training Programs
- OSHA Carcinogens Topics Webpage
- OSHA Hazardous and Toxic Substances Topics Webpage
- International Agency for Research on Cancer Monographs
- Reproductive Toxins Known to the State of California through Prop 65
- Institutional Biosafety committee
- University Committee on Human Subjects
- Compliance with Government Regulations
- Safety in Academic Chemistry Laboratories
- Safety Data Sheets Webpage
- Reproductive Hazard Assessment Form
9.1 Establishment of a Designated Area9.1 Establishment of a Designated Area
For work involving particularly hazardous substances, laboratories should establish a designated area where particularly hazardous substances can only be used. In some cases, a designated area could be an entire room out of a suite of rooms, or could mean one particular fume hood within a laboratory. The idea is to designate one area that everyone in the laboratory is aware of where the particularly hazardous substances can only be used.
In certain cases of establishing designated areas, Principal Investigators and laboratory supervisors may want to restrict use of a particularly hazardous substance to a fume hood, glove box or other containment device. This information should be included as part of the laboratory’s SOPs and covered during in-lab training.
Establishing a designated area not only provides better employee protection, but can help minimize the area where potential contamination of particularly hazardous substances could occur. If a designated area is established, a sign should be hung up (on a fume hood for example) indicating the area is designated for use with particularly hazardous substances. Most designated areas will have special PPE requirements and/or special waste and spill cleanup procedures as well. These and other special precautions should be included within the lab’s SOPs.
9.2 Safe Removal of Contaminated Materials and Waste9.2 Safe Removal of Contaminated Materials and Waste
Some particularly hazardous substances may require special procedures for safe disposal of both waste and/or contaminated materials. When in doubt, contact EHS at askEHS@cornell.edu to determine proper disposal procedures. Once these disposal procedures have been identified, they should be included as part of the laboratory’s SOPs and everyone working in the lab should be trained on those procedures.
9.3 Decontamination Procedures9.3 Decontamination Procedures
Some particularly hazardous substances may require special decontamination or deactivation procedures (such as Diaminobenzidine waste or Ethidium bromide) for safe handling. Review SDSs and other reference materials when working with particularly hazardous substances to identify is special decontamination procedures are required. If they are required, then this information should be included in the laboratory’s SOPs and appropriate training needs to be provided to laboratory personnel who work with these chemicals.
9.4 Guidelines for Working with Particularly Hazardous Substances9.4 Guidelines for Working with Particularly Hazardous Substances
Laboratory staff should always practice good housekeeping, use engineering controls, wear proper PPE, develop and follow SOPs, and receive appropriate training when working with any chemicals. The following special guidelines should be adhered to when working with particularly hazardous substances:
- Substitute less hazardous chemicals if possible to avoid working with particularly hazardous substances and keep exposures to a minimum.
- Always obtain prior approval from the Principal Investigator before ordering any particularly hazardous substances.
- Plan your experiment out in advance, including layout of apparatus and chemical and waste containers that are necessary.
- Before working with any particularly hazardous substance, review chemical resources for any special decontamination/deactivation procedures and ensure you have the appropriate spill cleanup materials and absorbent on hand.
- Ensure that you have the appropriate PPE, particularly gloves (check glove selection charts or contact EHS at askEHS@cornell.edu.
- Always use the minimum quantities of chemicals necessary for the experiment. If possible, try adding buffer directly to the original container and making dilutions directly.
- If possible, purchase premade solutions to avoid handling powders. If you have to use powders, it is best to weigh them in a fume hood. If it is necessary to weigh outside of a fume hood (because some particles may be too light and would pose more of a hazard due to turbulent airflow) then wear a dust mask when weighing the chemical. It is advisable to surround the weighing area with wetted paper towels to facilitate cleanup.
- As a measure of coworker protection when weighing out dusty materials or powders, consider waiting until other coworkers have left the room to prevent possible exposure and thoroughly clean up and decontaminate working surfaces.
- Whenever possible, use secondary containment, such as trays, to conduct your experiment in and for storage of particularly hazardous substances.
- Particularly hazardous substances should be stored by themselves in clearly marked trays or containers indicating what the hazard is i.e. “Carcinogens,” Reproductive Toxins”, etc.
- Always practice good personal hygiene, especially frequent hand washing, even if wearing gloves.
- If it is necessary to use a vacuum for cleaning particularly hazardous substances, only High Efficiency Particulate Air (HEPA) filters are recommended for best capture and protection. Be aware that after cleaning up chemical powders, the vacuum bag and its contents may have to be disposed of as hazardous waste.
- Ensure information related to the experiment is included within any SOPs.
9.5 Prior Approval9.5 Prior Approval
The OSHA Laboratory Standard requires Chemical Hygiene Plans to include information on “the circumstances under which a particular laboratory operation, procedure or activity shall require prior approval”, including “provisions for additional employee protection for work with particularly hazardous substances” such as "select carcinogens," reproductive toxins, and substances which have a high degree of acute toxicity.
Prior approval ensures that laboratory workers have received the proper training on the hazards of particularly hazardous substances or with new equipment, and that safety considerations have been taken into account BEFORE a new experiment begins.
Principal Investigators or laboratory supervisors must identify operations or experiments that involve particularly hazardous substances (such as "select carcinogens," reproductive toxins, and substances which have a high degree of acute toxicity) and highly hazardous operations or equipment that require prior approval. They must establish the guidelines, procedures, and approval process that would be required. This information should be documented in the laboratory's or department's SOPs. Additionally, Principal Investigators and laboratory supervisors are strongly encouraged to have written documentation, such as “Prior Approval” forms that are completed and signed by the laboratory worker, and signed off by the Principal Investigator or laboratory supervisor and kept on file.
Examples where Principal Investigators or laboratory supervisors should consider requiring their laboratory workers to obtain prior approval include:
- Experiments that require the use of particularly hazardous substances such as "select carcinogens," reproductive toxins, and substances that have a high degree of acute toxicity, highly toxic gases, cryogenic materials and other highly hazardous chemicals or experiments involving radioactive materials, high powered lasers, etc.
- Where a significant change is planned for the amount of chemicals to be used for a routine experiment such as an increase of 10% or greater in the quantity of chemicals normally used.
- When a new piece of equipment is brought into the lab that requires special training in addition to the normal training provided to laboratory workers.
- When a laboratory worker is planning on working alone on an experiment that involves highly hazardous chemicals or operations.
9.6 Campus Prior Approval9.6 Campus Prior Approval
There are some circumstances where prior approval from a campus research related committee is required before beginning an operation or activity. These include:
- Research using live vertebrate animals:
- contact the Institutional Animal Care and Use Committee at 607-253-3516.
- Recombinant DNA use:
- contact the Institutional Biosafety Committee at 607-255-5013.
- Use of Radioactive Materials:
- contact the EHS Radiation Safety Group at 607-255-8200.
- Use of Human Subjects:
- contact the University Committee on Human Subjects at 607-255-5138.
- Purchases involving Poison Inhalation Hazards:
Additional information can be obtained from the Office of Research and Integrity Assurance webpage for Compliance with Government Regulations.
9.7 Select Carcinogens9.7 Select Carcinogens
A carcinogen is any substance or agent that is capable of causing cancer – the abnormal or uncontrolled growth of new cells in any part of the body in humans or animals. Most carcinogens are chronic toxins with long latency periods that can cause damage after repeated or long duration exposures and often do not have immediate apparent harmful effects.
The OSHA Lab Standard defines a “select carcinogen” as any substance which meets one of the following criteria:
- It is regulated by OSHA as a carcinogen; or
- It is listed under the category, "known to be carcinogens," in the Annual Report on Carcinogens published by the National Toxicology Program (NTP) (latest edition); or
- It is listed under Group 1 ("carcinogenic to humans") by the International Agency for Research on Cancer Monographs (IARC) (latest editions); or
- It is listed in either Group 2A or 2B by IARC or under the category, "reasonably anticipated to be carcinogens" by NTP, and causes statistically significant tumor incidence in experimental animals in accordance with any of the following criteria:
- (A) After inhalation exposure of 6-7 hours per day, 5 days per week, for a significant portion of a lifetime to dosages of less than 10 mg/m(3);
- (B) After repeated skin application of less than 300 (mg/kg of body weight) per week; or
- (C) After oral dosages of less than 50 mg/kg of body weight per day.
With regard to mixtures, OSHA requires that a mixture “shall be assumed to present a carcinogenic hazard if it contains a component in concentrations of 0.1% or greater, which is considered to be carcinogenic.” When working with carcinogens, laboratory staff should adhere to Guidelines for Working with Particularly Hazardous Substances.
Note that the potential for carcinogens to result in cancer can also be dependent on other “lifestyle” factors such as:
- Cigarette smoking
- Alcohol consumption
- Consumption of high fat diet
- Geographic location – industrial areas and UV light exposure
- Therapeutic drugs
- Inherited conditions
More information on carcinogens, including numerous useful web links such as a listing of OSHA regulated carcinogens, can be found on the OSHA Safety and Health Topics for Carcinogens webpage. The State of California has developed an extensive list of “Carcinogens Known to the State of California through Prop 65”. Please note, this list is being provided as supplemental information to the OSHA, NTP and IARC chemical lists and is not legally mandated by New York State.
9.8 Reproductive Toxins9.8 Reproductive Toxins
A reproductive toxin is a substance or agent that can cause adverse effects on the reproductive system. The toxic effects may include alterations to the reproductive organs and/or to the endocrine system (which includes the thyroid and adrenal glands). These effects can occur in both men and women.
It is important to know what chemicals that are utilized in various processes. A number of reproductive toxins are chronic toxins that cause damage after repeated or long duration exposures and can have long latency periods. Women of childbearing potential and persons planning families should be especially careful when handling reproductive toxins. Pregnant women and persons intending to start families should seek the advice of their physician before working with known or suspected reproductive toxins.
EHS offers free and confidential consultation services for personnel with concerns associated with their workspace, procedures and PPE associated with working with the reproductive toxins and can be requested here or from the Reproductive Health webpage.
The following precautions should be taken when working with potentially toxic materials:
- Wear proper protective equipment including gloves, goggles and a lab coat that covers street clothes
- Gloves should be selected based on the properties of the chemicals in use
- Do not eat, drink, chew gum or apply cosmetics in the area toxic chemicals are being used
- Keep accurate records of amounts of these substances used
- Have a plan, proper equipment and materials ready to minimize exposure if an accident is to occur
- Procedure should be done with the minimum amount of material needed to complete the task
- All procedures should take place in a "controlled area" that is clearly labeled with a warning and restrictive access sign
- Whenever possible, fume hoods, glove boxes and isolation cabinets should be used, if these are not feasible, proper respiratory protection must be worn
- All work surfaces should be easily cleanable and covered in an impervious or disposable material
- Wash hands, arms and decontaminate work surface and equipment thoroughly when done with the procedure
- Protective apparel worn while working with toxic materials should not be worn outside the laboratory
- Ensure that containers of contaminated waste are transferred from controlled area in a secondary container to avoid further contamination
- Obtain SDS and procedure information for medical provider if they have any questions.
9.9 Acute Toxins9.9 Acute Toxins
OSHA defines a chemical as being highly toxic if it falls within any of the following categories:
- A chemical that has a median lethal dose (LD50) of 50 milligrams or less per kilogram of body weight when administered orally to albino rats weighing between 200 and 300 grams each.
- A chemical that has a median lethal dose (LD50) of 200 milligrams or less per kilogram of body weight when administered by continuous contact for 24 hours (or less if death occurs within 24 hours) with the bare skin of albino rabbits weighing between two and three kilograms each.
- A chemical that has a median lethal concentration (LC50) in air of 200 parts per million by volume or less of gas or vapor, or 2 milligrams per liter or less of mist, fume, or dust, when administered by continuous inhalation for one hour (or less if death occurs within one hour) to albino rats weighing between 200 and 300 grams each.
As with any particularly hazardous substance, work involving the use of acute toxins should adhere to the Guidelines for Working with Particularly Hazardous Substances. In addition to following the Guidelines for Working with Particularly Hazardous Substances, additional guidelines for working with acute toxins include:
- Consider storing highly toxic materials in a locked storage cabinet.
- Be aware of any special antidotes that may be required in case of accidental exposure (Hydrofluoric acid and inorganic cyanides for example).
- Give particular attention to the selection of gloves and other personal protective equipment.
- Do not work with highly toxic chemicals outside of a fume hood, glove box or ventilated enclosure.
More information on acute toxins, including numerous useful web links, can be found on the OSHA Safety and Health Topics for Hazardous and Toxic Substances webpage.
Chapter 10 - Hazardous Chemical Waste DisposalChapter 10 - Hazardous Chemical Waste Disposal
Hazardous chemical waste storage and disposal is regulated by the U.S. Environmental Protection Agency (EPA). In New York State, the Department of Environmental Conservation (DEC) regulates chemical waste management activities. All University chemical wastes are subject to inspection and enforcement actions by the EPA or the DEC. EHS provides the following chemical waste compliance services:
- Management of University main hazardous waste accumulation area.
- Collection of chemical waste.
10.1 Hazardous Chemical Waste Container Requirements10.1 Hazardous Chemical Waste Container Requirements
Within your work area, the following practices must be followed for proper management of hazardous waste:
- Determine if your unwanted materials pose a significant risk requiring management as hazardous waste.
- Determine if chemical deactivation or drain disposal is an option.
- Label containers of hazardous chemical wastes with the identity of the chemical(s) AND the words “Hazardous Waste” or label with a Cornell University Hazardous Waste label.
- Keep containers of hazardous chemical wastes closed at all times when they are not in use.
- Store hazardous waste containers within the room in which they are generated in.
- Recommended practices that should be followed:
- Always maintain a neat and orderly workplace.
- Use secondary containment bins or trays to store your chemical waste containers in.
- Store your waste containers in a designated place.
10.2 Hazardous Waste Pickup Procedures10.2 Hazardous Waste Pickup Procedures
There are a variety of hazardous wastes that contain mixed Regulated Medical Waste and chemical Hazardous Waste. Follow these procedures to prepare laboratory generated wastes.
Waste Determination Flow Chart
Laboratory Waste Disposal Guide
To have your hazardous waste picked up by EHS, please complete the following procedures:
Place an EHS Hazardous Waste label on each container.
- Labels are available by calling 607-255-8200 or askEHS@cornell.edu.
- Fill out the waste label and list all of the ingredients (no trade names or chemical formulas, please). Do not put a date on the tag until you are ready to dispose of the waste through EHS.
- Peel off the bottom copy and stick it on the waste container.
- Place the waste containers in a DOT compliant box (has a U/N symbol on it). Place the top copy of the waste label inside the box.
- Request a waste pick up by filling out the online request for chemical waste removal.
Boxes are available from:
Bard, Kimball, Thurston waste generators: Contact the Building Coordinator at 607-255-3579. Boxes are stored in room B60A, Bard Hall.
- Biotech/Weill Hall waste generators: Boxes are located in the gas cylinder storage room in the basement between the buildings. Contact the Biotech Building Coordinator at 607-254-4583.
- Clark Hall waste generators: Contact the Building Coordinator, phone 607-255-3524. Boxes are kept in stock room on ground floor.
- Comstock Hall waste generators: Contact the Building Coordinator.
- Corson-Mudd Hall waste generators: Contact the Building Coordinator, phone 607-254-4395.
- Emerson-Bradfield Hall waste generators: Boxes can be obtained from the mail room in G03, Bradfield Hall.
- J.A. Baker waste generators: Boxes are stored in room B18, basement near the elevator.
- Martha Van Rensselaer waste generators: Boxes are stored at the loading dock in the sub-basement.
- Olin Hall: Contact the Building Coordinator, phone 607-255-3540.
- Plant Science waste generators: Contact the Building Coordinator, phone 607-255-7826. Boxes are stored at loading dock.
- Vet College Complex waste generators, including Schurman Hall, Vet Research Tower and Vet Medical Center. Boxes can be obtained from the 1st floor Custodial Supply area - please see staff in the mail room (Vet Medical Center) for access to the storage facility.
The following types of materials have different requirements for disposal:
- Construction debris (such as asbestos and lead)
- CRTs (computer monitors and televisions)
- Radioactive materials
- Scrap electronics (circuit boards)
- Used oils
- Universal Waste (Fluorescent bulbs, batteries)
Chapter 11 - Hazardous Material ShippingChapter 11 - Hazardous Material Shipping
The transport of hazardous materials is regulated by the U.S. Department of Transportation (DOT) and the International Civil Aviation Organization. An additional regulatory body formed by the airlines is called the International Air Transport Association (IATA); however, their regulations are only enforceable by the airlines. All University transportation (including shipping) of hazardous materials off University owned property is subject to DOT enforcement.
EHS provides the following hazardous material shipping compliance services:
- Compliance training
- Maintenance of training records
- University DOT certification program
- General compliance assistance
- Oversight of shipping stations (where untrained/uncertified individuals may have a hazardous material shipped for them)
11.1 Regulated Hazardous Materials11.1 Regulated Hazardous Materials
The following materials are regulated as hazardous materials for transportation:
- Alcohol solutions
- Compressed gases
- Dry Ice (air shipments only)
- Flammable liquids and solids
- Formaldehyde - solutions between 0.1% and 25% (air shipments only)
- Infectious substances (animals and humans only)
- Radioactive materials
11.2 Hazardous Materials Transportation Requirements11.2 Hazardous Materials Transportation Requirements
The following must be adhered to for all shipments involving hazardous materials:
- All shipments of hazardous materials from the University must be prepared by a trained and Cornell University certified individuals. Anyone who classifies, packages, marks, or labels hazardous materials for shipment must be a trained and Cornell University certified individual.
- Transport of hazardous materials by Cornell University members for University business requires the preparer and the driver to be trained and Cornell University certified.
- Shipments of hazardous materials received by Cornell University do not require a trained and Cornell University certified individual unless they help unload the truck.
For any other questions, please email the Hazardous Materials Shipping Program Coordinator at askEHS@cornell.edu or call 607-255-8200.
Chapter 12 - PesticidesChapter 12 - Pesticides
A pesticide is defined as a substance or mixture of substances intended for preventing, destroying, repelling or mitigating any pest, or intended for use as a plant regulator, defoliant or desiccant. Two categories of pesticides are covered by the Cornell University Health and Safety Policy 8.6:
- EPA Registered Pesticides (the EPA registration number can be found on the manufacturer's label).
- Those experimental chemicals for which a pesticidal effect has been determined.
All university personnel (includes faculty members, staff members, students, and any other university-affiliated individuals) who label, store, use, transport, dispose of, or clean up spills of pesticides are responsible for adhering to federal and state regulations, as well as the Cornell University Health & Safety Policy 8.6.
It is essential that teaching, research, extension, ground, and athletic field maintenance involving pesticide use be conducted properly and legally for the protection of the pesticide applicator, other employees, staff, students, public health, and the environment.
- OSHA Lab Standard
- University Health and Safety Policy 8.6
- NYS Environmental Conservation Law Part 325
- Pesticide Regulations - 40 CFR part 172.3
- CALS Pesticide Website
12.1 Pesticide Certification12.1 Pesticide Certification
Cornell University requires that all individuals handling pesticides as a part of university programs must be NYS certified pesticide applicators. For more information on this requirement, see the Cornell University Health and Safety Policy 8.6.
12.1.1 Exemptions from Pesticide Certification12.1.1 Exemptions from Pesticide Certification
As per state and federal regulations, a number of exemptions from pesticide certification requirements exist.
These exemptions include:
- Licensed veterinarians, as well as licensed veterinary technicians, interns, residents, and veterinary students working under the direct supervision of a veterinarian in a veterinary facility (any building operated by the College of Veterinary Medicine) are exempt from the certification requirement when engaged in the use of general-use pesticides.
- Small laboratory quantities of pesticides used for analysis and treatment of samples in a laboratory and in an environmentally non-dispersive manner (e.g., microgram or gram quantities used inside a fume hood, mixed into media, etc.) are exempt from policy requirements. As with all other chemical use in the laboratory, use of laboratory quantity pesticides is regulated by the OSHA Laboratory Standard and other appropriate rules and regulations.
- Testing of materials for pesticide efficacy, toxicity, or other properties could also be exempted - for clarification, contact the Occupational and Environmental Health Program, College of Agriculture and Life Sciences at 607-255-0485, or refer to the federal regulation: 40 CFR part 172.3.
- Teaching/demonstration of pesticide application(s), as well as recommendation of pesticide application or use is exempted from the certification requirements. However, the individual engaged in such activities is responsible for ensuring that these activities are compliant with federal, state, and local pesticide laws and regulations.
For more information regarding pesticide use requirements and exemptions, please refer to the Cornell University Health and Safety Policy 8.6 or contact the CALS Occupational and Environmental Health Program at firstname.lastname@example.org, or 607-255-0485, or visit the CALS Pesticide Webpage.
Chapter 13 - BiohazardsChapter 13 - Biohazards
Information about how biosafety is managed at Cornell can be found in the Biological Safety Manuals on this web site.
Chapter 14 - Radiation HazardsChapter 14 - Radiation Hazards
Ionizing radiation is a form of energy. Unlike some other types of energy, such as heat (infrared radiation) or visible light, the human body cannot sense exposure to ionizing radiation. Nonetheless, absorption of ionizing radiation energy by body tissues causes changes to the chemical makeup of living cells.
The type and thickness of material needed to make an effective barrier or shield around a source of ionizing radiation varies a great deal depending on the type of ionizing radiation. Beta radiation is a stream of tiny charged particles that can be stopped by a thin layer of plastic, glass, wood, metal and most other common materials. X-rays and Gamma rays are very similar to sunlight in that they are not particles, just electromagnetic waves. While sunlight will pass through only a few materials, such as window glass, X-rays and Gamma rays penetrate easily through most materials. However, even they can be blocked by a sufficient thickness of lead.
Ionizing radiation is also similar to other forms of radiation in that the intensity of the radiation exposure decreases very quickly as you move away from the radiation source. Just as moving a short distance closer to or farther from a fireplace causes a large change in how warm you feel; keeping just a few feet away from where someone is handling radioactive material will almost eliminate your exposure.
- Cornell University Radiation Safety Manual
- Disposal of Radioactive Materials
- Cornell Radiation Safety Program
14.1 Where Ionizing Radiation is Used14.1 Where Ionizing Radiation is Used
Small amounts of radioactive material are used and stored in hundreds of laboratory rooms around the campus. Some of the material is contained in small sealed capsules. Examples of these “sealed sources” include test sources for radiation detectors and ionization detectors in gas chromatographs. Most often radioactive material is found in small vials of radioactively labeled chemicals in solution. These labeled chemicals are widely used in research and in veterinary medicine. With very few exceptions, only very small amounts of radioactive material are used and levels of radiation exposure are quite low.
Ionizing radiation can also be produced by certain electrical equipment, including X-ray machines and particle accelerators. There are approximately one hundred pieces of radiation producing equipment on the campus. Radiation levels produced by this equipment are also very low because of shielding.
You can tell if a room contains a source of ionizing radiation because each entrance is plainly marked by warning labels. Within the room, additional labels and warning tape will be found on each piece of radiation producing equipment and on all areas used to work with or store radioactive material.
14.2 Potential Hazards14.2 Potential Hazards
Like any form of energy, ionizing radiation can be harmful if a person is exposed to an excessive amount. Exposure to ionizing radiation causes chemical damage to body tissues and can be harmful. Just as with exposure to any toxic chemical, the human body can tolerate exposure to ionizing radiation up to a point without producing any immediate injury. However, just as with toxic chemicals, high levels of exposure can cause serious injuries including skin burns, hair loss, internal bleeding, anemia and immune system suppression. In addition, exposure to high levels of ionizing radiation has been proven to cause an increased lifetime risk of cancer.
14.2.1 How to Protect Yourself14.2.1 How to Protect Yourself
Responsibility for protecting themselves, co-workers and others from exposure to ionizing radiation is delegated by the Radiation Safety Committee to the Principal Investigator or area supervisor and to each of the individual users. Appropriate safety requirements, that are specific to each use and location, are written into each approval granted by the Committee. Every user is trained in radiation safety principles and on the specific safety requirements of their operations before they are allowed to begin working with radioactive material.
Other individuals in these areas, who are not trained to use radioactive material or radiation producing equipment, need to follow the safety procedures established for those working with ionizing radiation. Primarily this means:
- Never operate equipment that produces ionizing radiation.
- Never handle items or containers that are labeled with radioactive material warnings or that are within areas marked as storage or use areas for radioactive material.
14.3 Control of Ionizing Radiation14.3 Control of Ionizing Radiation
All use of material or equipment that produces ionizing radiation requires prior approval by the Cornell University Radiation Safety Committee. This group of faculty members set policies and personally reviews each operation to ensure safety and compliance with state and federal regulations. The University Radiation Safety Officer and the Radiation Safety Group within EHS provide training and other services to help individuals work safely. In addition, they perform routine inspections of all use areas and require correction of all violations of radiation safety requirements. Detailed information on the university radiation safety program is available in the Cornell University Radiation Safety Manual.
The performance of the Cornell Radiation Safety Program is reviewed continuously. The Radiation Safety Committee meets 4 times each year to keep policies up-to-date, resolve problems and compliance issues and to monitor the level of radiation exposure to individuals on campus. Historically, less than 1% of the individuals using ionizing radiation at Cornell receive more than 1% of the annual allowable dose limit. The Committee also audits EHS programs and services. In addition, the New York State Department of Health performs an on campus assessment of our program every two years.
The information presented here is only a brief overview of how sources of ionizing radiation are used at Cornell University. While Cornell has demonstrated that it has a solid and consistent safety program, it is important not to take safety for granted. If you have questions or concerns about the use of ionizing radiation where you work, you are entitled to answers and information. The Principal Investigator, area supervisor or any authorized user is willing and able to help you and you should feel free to speak with them. They understand that many individuals have never had formal training about radiation safety. If you need additional assistance or have any other questions, please contact EHS at 607-255-8200 or askEHS@cornell.edu.
14.4 Radioactive Waste Disposal14.4 Radioactive Waste Disposal
Radioactive material cannot be disposed of in the regular trash. Please see the Radioactive Waste page for procedures to prepare radioactive waste for collection by EHS. For more information or if you have any questions, please contact askEHS.
Chapter 15 - Laser HazardsChapter 15 - Laser Hazards
Cornell University has a Laser Safety Program designed to establish guidelines to protect students and employees from the potential hazards associated with laser devices and systems used to conduct laboratory, educational, or research activities at Cornell University. To achieve this goal, EHS recognizes the American National Standard for the Safe Use of Lasers, ANSI Z136.1-2000 and New York Department of Labor’s Part 50, LASER Regulation.
ANSI Z136.1-2007 requires that all class 3b and 4 laser users must attend laser safety training. EHS offers training to meet this requirement, which includes topics such as laser hazards, laser classifications, signage/labeling, medical monitoring, safety guidelines, and what to do in case of an exposure incident. A description of the training can be found on the EHS Safety Education Catalog. The Laser Safety Training class is offered on a monthly basis by submitting a request to askEHS@cornell.edu.
Additionally, any class 3b and 4 lasers that are in use must be registered with EHS. If your user group has not completed this process, please complete the LASER registration form and return it to EHS at 395 Pine Tree Road, Suite 210. Laser safety reviews, control recommendations, and medical monitoring are also available by submitting a request to askEHS@cornell.edu.
For additional information regarding laser safety please contact the EHS Laser Safety Officer as askEHS@cornell.edu or call 607-255-8200. Additional information can be found on the OSHA Safety and Health topics webpage for laser hazards.
Chapter 16 - Physical HazardsChapter 16 - Physical Hazards
In addition to the chemical hazards found in laboratories, there are also numerous physical hazards encountered by laboratory staff on a day-to-day basis. As with chemical hazards, having good awareness of these hazards, good preplanning, use of personal protective equipment and following basic safety rules can go a long way in preventing accidents involving physical hazards.
16.1 Electrical Safety16.1 Electrical Safety
Electricity travels in closed circuits, and its normal route is through a conductor. Shock occurs when the body becomes a part of the electric circuit. Electric shock can cause direct injuries such as electrical burns, arc burns, and thermal contact burns. It can also cause injuries of an indirect or secondary nature in which involuntary muscle reaction from the electric shock can cause bruises, bone fractures, and even death resulting from collisions or falls. Shock normally occurs in one of three ways.
The person must be in contact with ground and must contact with:
- Both wires of the electric circuit, or
- One wire of the energized circuit and the ground, or
- A metallic part that has become energized by being in contact with an energized wire.
The severity of the shock received when a person becomes a part of an electric circuit is affected by three primary factors:
- The amount of current flowing through the body (measured in amperes).
- The path of the current through the body.
- The length of time the body is in the circuit.
Other factors that may affect the severity of shock are the frequency of the current, the phase of the heart cycle when shock occurs, and the general health of the person prior to shock. The effects of an electrical shock can range from a barely perceptible tingle to immediate cardiac arrest. Although there are no absolute limits or even known values that show the exact injury from any given amperage, the table above shows the general relationship between the degree of injury and the amount of amperage for a 60-cycle hand-to-foot path of one second's duration of shock.
|1 Milliampere||Perception level. Just a faint tingle.|
|5 Milliamperes||Slight shock felt. Average individual can let go. However, strong involuntary reactions to shocks in this range can lead to injuries.|
|6-30 Milliamperes||Painful shock. Muscular control lost.|
|50-150 Milliamperes||Extreme pain, respiratory arrest, severe muscular contractions. Individual cannot let go. Death is possible.|
|1,000-4,300 Milliamperes||Ventricular fibrillation. Muscular contraction and nerve damage occur. Death is most likely.|
|10,000-Milliamperes||Cardiac arrest, severe burns and probable death.|
As this table illustrates, a difference of less than 100 milliamperes exists between a current that is barely perceptible and one that can kill. Muscular contraction caused by stimulation may not allow the victim to free himself/herself from the circuit, and the increased duration of exposure increases the dangers to the shock victim. For example, a current of 100 milliamperes for 3 seconds is equivalent to a current of 900 milliamperes applied for 0.03 seconds in causing fibrillation. The so-called low voltages can be extremely dangerous because, all other factors being equal, the degree of injury is proportional to the length of time the body is in the circuit. Simply put, low voltage does not mean low hazard.
In the event of an accident involving electricity, if the individual is down or unconscious, or not breathing: CALL Cornell University Police at 911 (607-255-1111 from a cell phone or off campus phone) immediately. If an individual must be physically removed from an electrical source, it is always best to eliminate the power source first (i.e.: switch off the circuit breaker) but time, or circumstance may not allow this option - be sure to use a nonconductive item such as a dry board. Failure to think and react properly could make you an additional victim. If the individual is not breathing and you have been trained in CPR, have someone call Cornell University Police and begin CPR IMMEDIATELY!
16.1.1 Common Electrical Hazards and Preventative Steps16.1.1 Common Electrical Hazards and Preventative Steps
Many common electrical hazards can be easily identified before a serious problem exists.
- Read and follow all equipment operating instructions for proper use. Ask yourself, "Do I have the skills, knowledge, tools, and experience to do this work safely?"
- Do not attempt electrical repairs unless you are a qualified electrical technician assigned to perform electrical work by your supervisor. Qualified individuals must receive training in safety related work practices and procedures, be able to recognize specific hazards associated with electrical energy, and be trained to understand the relationship between electrical hazards and possible injury. Fixed wiring may only be repaired or modified by Facilities Services.
- All electrical devices fabricated for experimental purposes must meet state and University construction and grounding requirements. Extension cords, power strips, and other purchased electrical equipment must be Underwriters Laboratories (UL) listed.
- Remove all jewelry before working with electricity. This includes rings, watches, bracelets, and necklaces.
- Determine appropriate personal protective equipment (PPE) based on potential hazards present. Before use, inspect safety glasses and gloves for signs of wear and tear, and other damage.
- Use insulated tools and testing equipment to work on electrical equipment. Use power tools that are double-insulated or that have Ground Fault Circuit Interrupters protecting the circuit. Do not use aluminum ladders while working with electricity; choose either wood or fiberglass.
- Do not work on energized circuits. The accidental or unexpected starting of electrical equipment can cause severe injury or death. Before any inspections or repairs are made, the current must be turned off at the switch box and the switch padlocked or tagged out in the off position. At the same time, the switch or controls of the machine or the other equipment being locked out of service should be securely tagged to show which equipment or circuits are being worked on. Test the equipment to make sure there is no residual energy before attempting to work on the circuit. Employees must follow the Cornell University lock-out/tag-out procedures.
- If you need additional power supply, the best solution is to have additional outlets installed by Facilities Services. Do not use extension cords or power strips ("power taps") as a substitute for permanent wiring.
- Extension cords and power strips may be used for experimental or developmental purposes on a temporary basis only. Extension cords can only be used for portable tools or equipment and must be unplugged after use. Do not use extension cords for fixed equipment such as computers, refrigerators/freezers, etc.; use a power strip in these cases. In general, the use of power strips is preferred over use of extension cords.
- Power strips must have a built-in overload protection (circuit breaker) and must not be connected to another power strip or extension cord (commonly referred to as daisy chained or piggy-backed). As mentioned above though, extension cords and power strips are not a substitute for permanent wiring.
- Ensure any power strips or extension cords are listed by a third-party testing laboratory, such as Underwriters Laboratory (UL). Make sure the extension cord thickness is at least as big as the electrical cord for the tool. For more information on extension cords, see the Consumer Product Safety Commission - Extension Cords Fact Sheet (CPSC Document #16).
- Inspect all electrical and extension cords for wear and tear. Pay particular attention near the plug and where the cord connects to the piece of equipment. If you discover a frayed electrical cord, contact your Building Coordinator for assistance. Do not use equipment having worn or damaged power cords, plugs, switches, receptacles, or cracked casings. Running electrical cords under doors or rugs, through windows, or through holes in walls is a common cause of frayed or damaged cords and plugs.
- Do not use 2-prong ungrounded electrical devices. All department-purchased electrical equipment must be 3-prong grounded with very limited exceptions.
- Never store flammable liquids near electrical equipment, even temporarily.
- Keep work areas clean and dry. Cluttered work areas and benches invite accidents and injuries. Good housekeeping and a well-planned layout of temporary wiring will reduce the dangers of fire, shock, and tripping hazards.
- Common scenarios that may indicate an electrical problem include: flickering lights, warm switches or receptacles, burning odors, sparking sounds when cords are moved, loose connections, frayed, cracked, or broken wires. If you notice any of these problems, have a qualified electrician address the issue immediately.
- To protect against electrical hazards and to respond to electrical emergencies it is important to identify the electrical panels that serve each room. Access to these panels must be unobstructed; a minimum of 3’ of clearance is required in front of every electrical panel. Each panel must have all the circuit breakers labeled as to what they control. Contact your Building Coordinator for assistance.
- When performing laboratory inspections, it is a good idea to verify the location of the power panel and to open the door to ensure any breakers that are missing have breaker caps in its place. If no breaker is present and no breaker cap is covering the hole, contact your Building Coordinator for assistance.
- Avoid operating or working with electrical equipment in a wet or damp environment. If you must work in a wet or damp environment, be sure your outlets or circuit breakers are Ground Fault Circuit Interrupter (GFCI) protected. Temporary GFCI plug adapters can also be used, but are not a substitute for GFCI outlets or circuit breakers.
Fuses, circuit breakers, and Ground-Fault Circuit Interrupters are three well-known examples of circuit protection devices.
- Fuses and circuit breakers are over-current devices that are placed in circuits to monitor the amount of current that the circuit will carry. They automatically open or break the circuit when the amount of the current flow becomes excessive and therefore unsafe. Fuses are designed to melt when too much current flows through them. Circuit breakers, on the other hand, are designed to trip open the circuit by electro-mechanical means.
- Fuses and circuit breakers are intended primarily for the protection of conductors and equipment. They prevent overheating of wires and components that might otherwise create hazards for operators.
- The Ground Fault Circuit Interrupter (GFCI) is designed to shut off electric power within as little as 1/40 of a second, thereby protecting the person, not just the equipment. It works by comparing the amount of current going to an electric device against the amount of current returning from the device along the circuit conductors. A fixed or portable GFCI should be used in high-risk areas such as wet locations and construction sites.
Entrances to rooms and other guarded locations containing exposed live parts must be marked with conspicuous warning signs forbidding unqualified persons to enter. Live parts of electric equipment operating at 50 volts or more must be guarded against accidental contact. Guarding of live parts may be accomplished by:
- Location in a room, vault, or similar enclosure accessible only to qualified persons.
- Use of permanent, substantial partitions or screens to exclude unqualified persons.
- Location on a suitable balcony, gallery, or platform elevated and arranged to exclude unqualified persons, or
- Elevation of 8 feet or more above the floor.
For additional information, see the following resources:
16.1.2 Safe Use of Electrophoresis Equipment16.1.2 Safe Use of Electrophoresis Equipment
Electrophoresis units present several possible hazards including electrical, chemical, and radiological hazards. All of these hazards need to be addressed before using the units. EHS has prepared these guidelines to assist researchers in safely operating electrophoresis units.
- Hazards associated with particular machines.
- How the safeguards provide protection and the hazards for which they are intended.
- How and why to use the safeguards.
- How and when safeguards can be removed and by whom.
- What to do if a safeguard is damaged, missing, or unable to provide adequate protection.
- Hazards to machine operators that can't be designed around must be shielded to protect the operator from injury or death. Guards, decals and labels which identify the danger must be kept in place whenever the machine is operated. Guards or shields removed for maintenance must be properly replaced before use. Moving parts present the greatest hazard because of the swiftness of their action and unforgiving and relentless motion
16.2 Machine Guarding16.2 Machine Guarding
Common machine hazards occurring around moving parts include:
Where two parts move together and at least one of the parts moves in a circle; also called mesh points, run-on points, and entry points. Examples include: Belt drives, chain drives, gear drives, and feed rolls.When shields cannot be provided, operators must avoid contact with hands or clothing in pinch point areas. Never attempt to service or unclog a machine while it is operating or the engine is running.
Any exposed component that rotates.Examples include: Rotating shafts such as a PTO shaft or shafts that protrude beyond bearings or sprockets. Watch components on rotating shafts, such as couplers, universal joints, keys, keyways, pins, or other fastening devices. Splined, square, and hexagon-shaped shafts are usually more dangerous than round shafts because the edges tend to grab fingers or clothing more easily than a round shaft, but round shafts may not be smooth and can also grab quickly. Once a finger, thread, article of clothing, or hair is caught it begins to wrap; pulling only causes the wrap to become tighter.
Where the edges of two moving parts move across one another or where a single sharp part moves with enough speed or force to cut soft material. Remember that crop cutting devices cannot be totally guarded to keep hands and feet out and still perform their intended function. Recognize the potential hazards of cutting and shear points on implements and equipment that are not designed to cut or shear. Guarding may not be feasible for these hazards.
Points that occur between two objects moving toward each other or one object moving toward a stationary object. Never stand between two objects moving toward one another. Use adequate blocking or lock-out devices when working under equipment.
Points where objects are pulled into equipment, usually for some type of processing. Machines are faster and stronger than people. Never attempt to hand-feed materials into moving feed rollers. Always stop the equipment before attempting to remove an item that has plugged a roller or that has become wrapped around a rotating shaft. Remember that guards cannot be provided for all situations - equipment must be able to function in the capacity for which it is designed. Freewheeling parts, rotating or moving parts that continue to move after the power is shut off are particularly dangerous because time delays are necessary before service can begin. Allow sufficient time for freewheeling parts to stop moving. Stay alert! Listen and Watch for Motion!
Any object that can become airborne because of moving parts. Keep shields in place to reduce the potential for thrown objects. Wear protective gear such as goggles to reduce the risk of personal injury if you cannot prevent particles from being thrown. All guards, shields or access doors must be in place when equipment is operating. Electrically powered equipment must have a lock-out control on the switch or an electrical switch, mechanical clutch or other positive shut-off device mounted directly on the equipment. Circuit interruption devices on an electric motor, such as circuit breakers or overload protection, must require manual reset to restart the motor.
16.3 Lighting16.3 Lighting
Having a properly lighted work area is essential to working safely. A couple of key points to remember about proper lighting:
- Lighting should be adequate for safe illumination of all work areas (100-200 lumens for laboratories). For more information, see the CU Design and Construction Standard 26500 – Interior Lighting.
- Light bulbs that are mounted low and susceptible to contact should be guarded.
- If the risk of electrocution exists when changing light bulbs, practice lock-out tag-out.
- For proper disposal of "universal waste" fluorescent bulbs, see light bulb recycling.
- As an energy conservation measure, please remember to turn off your lights when you leave your lab.
16.4 Compressed Gases16.4 Compressed Gases
Compressed gases are commonly used in laboratories.
- Gas cylinders may contain gases that are flammable, highly toxic, toxic, corrosive, asphyxiant, or oxidizing.
- A risk assessment, such as a POSHER (docx) review, may determine that a gas cabinet, leak detection, and an emergency shut off button in the hallway may be required.
- Unsecured cylinders can be tipped over, causing serious injury and damage. Impact can shear the valve from an uncapped cylinder, causing a catastrophic release of pressure leading to personal injury and extensive damage.
- Mechanical failure of the cylinder, cylinder valve, or regulator can result in rapid diffusion of the pressurized contents of the cylinder into the atmosphere; leading to explosion, fire, runaway reactions, or burst reaction vessels.
- A minimum 1 cubic foot/minute/square foot (cfm/ft2) of room ventilation is required for the storage and use of compressed gases due to the hazards listed in this chapter.
- In accordance with NFPA 704, gases or liquids having a hazard ranking of Health Class 3 or 4, Flammability Class 4, or Instability Class 3 or 4 that are carried in pressurized piping above 15 pounds per square inch gauge (psig) (103 kPa), an approved means of leak detection and emergency shutoff or excess flow control shall be provided.
Backflow prevention or check valves shall be provided where the backflow of the hazardous gas could create a hazardous condition or cause the unauthorized discharge of hazardous materials.
Piping, tubing, valves, fittings and related components shall be designed and fabricated from materials that are compatible with the material to be contained and shall be of adequate strength and durability to withstand the pressure, structural and seismic stress and exposure to which they are subject.
Use a restrictive flow orifice (RFO) or needle valve to restrict flow to only that needed for the experiment. Consider using a dilution of the gas that is suitable for the research, but falls outside of the code requirements.
- Manual valves or automatic remotely activated fail-safe emergency shutoff valves shall be installed on supply piping and tubing and provided with ready access at the following locations:
- The point of use.
- The tank, cylinder or bulk source.
Manual emergency shutoff valves and controls for remotely activated emergency shutoff valves shall be identified and the location shall have access clearly visible and indicated by means of a sign.
- Template (docx) for writing a Standard Operating Procedure- Use this template for developing protocols a specific hazardous gas system.
16.4.1 Safe Storage and Handling of Compressed Gas Cylinders16.4.1 Safe Storage and Handling of Compressed Gas Cylinders
- Restrain cylinders of all sizes by straps, chains, or a suitable stand to prevent them from falling;
- Keeps cylinders in storage upright, secure, and interlocked into a compact group;
- Do not use compressed gases in occupiable environmental chambers, cold rooms, or other similar stand-alone units. These typically do not provide outside air ventilation;
- Keep cylinders capped and the regulator off when not in use;
- Keep cylinders labeled with a full/in-use/empty tag.
- The contents of any compressed gas cylinder must be clearly identified. Labels on cylinders may be stenciled, stamped, or a label or tag attached to the cylinder. Do not rely on the color of the cylinder for identification because color-coding is not standardized and may vary with the manufacturer or supplier.
- For short term experiments using hazardous gases, always select the smallest returnable cylinder available. In cases where the gas will be used over an extended period of time (several months to more than one year), you should order a gas quantity that will last for three to six months;
- Some small cylinders, such as lecture bottles and cylinders of highly toxic gases, are not fitted with rupture devices and may explode if exposed to high temperatures;
- Do not store or use incompatible gases next to each other;
- Cylinders of oxygen must be stored at least 20 feet away from cylinders of any flammable gas.
- Isolate them by storing in a gas cabinets.
- Segregate full cylinders of low hazard gases from "empty" cylinders awaiting return to the vendor;
- Do not expose cylinders to temperatures higher than 50 ˚C (125 ˚F);
- Never place cylinders where they may become part of an electric circuit;
- Corrosive gases should be returned to the gas supplier within one year to avoid regulator and cylinder valve problems due to corrosion;
- Avoid areas that are damp or subject to other corrosive materials;
- When transporting cylinders:
- Always use a hand truck equipped with a chain or belt for securing the cylinder. Full size cylinders weigh up to 300 pounds.
- Make sure the protective cap covers the cylinder valve.
- Never transport a cylinder while a regulator is attached.
- Avoid riding in elevators with compressed gas cylinders. If this is necessary, consider using a buddy system to have one person send the properly secured cylinders on the elevator, while the other person waits at the floor by the elevator doors where the cylinders will arrive.
- Do not move compressed gas cylinders by carrying, rolling, sliding, or dragging them across the floor.
- Do not transport oxygen and combustible gases at the same time.
- Do not drop cylinders or permit them to strike anything violently.
16.4.2 Fume Hood Applications16.4.2 Fume Hood Applications
Fume hood applications: This does not apply to highly toxic and toxic compressed gases, in cylinders large than 20ft3 at STP due to the face velocity requirements.
- The smallest possible cylinder should be used for the experiment (a six-month bottle supply for routine use gases is appropriate, while smaller cylinder supplies are suggested for short term use);
- Cylinders of toxic and highly toxic gases that are 20 ft3 or smallerat NTP are allowed within fume hoods as long as there are no incompatible materials also used in the hood or storage of any items inside.
- Make an effort to obtain gas cylinders in returnable bottles;
- Order bottles with lowest cylinder pressure possible;
- Use a flow restricting orifice or needle valve to restrict flow to only that needed for the experiment;
- Consider using a dilution of the gas that is suitable for the research, but falls outside of the New York State code requirements;
- Place the cylinder in rear of the hood. High pressure leaks can readily escape the hood and capture is best in the rear of the hood;
- Assure all components in experiment can withstand full bottle pressure or incorporate pressure relief (run relief line into a hood slot);
- All gas lines connected to the hazardous gas source, including purge lines and gas supply lines, must be completely contained inside of the hood. If this is not possible, the cylinder must go into a gas cabinet.
16.4.3 Operation of Compressed Gas Cylinders16.4.3 Operation of Compressed Gas Cylinders
The cylinder valve hand wheel opens and closes the cylinder valve. The pressure relief valve is designed to keep a cylinder from exploding in case of fire or extreme temperature. Cylinders of very toxic gases do not have a pressure relief valve, but they are constructed with special safety features. The valve outlet connection is the joint used to attach the regulator. The pressure regulator is attached to the valve outlet connector in order to reduce the gas flow to a working level. The Compressed Gas Association has intentionally made certain types of regulators incompatible with certain valve outlet connections to avoid accidental mixing of gases that react with each other. Gases should always be used with the appropriate regulator. Do not use adaptors with regulators. The cylinder connection is a metal-to-metal pressure seal. Make sure the curved mating surfaces are clean before attaching a regulator to a cylinder. Do not use Teflon tape on the threaded parts, because this may actually cause the metal seal not to form properly. Always leak test the connection.
Here is a link to the Airgas material compatibility chart: http://airgassgcatalog.com/catalog/ap020.pdf as well as the available excess flow valves: http://airgassgcatalog.com/catalog/E113_TAG_096.pdf
Basic Operating guidelines include:
- Make sure that the cylinder is secured.
- Attach the proper regulator to the cylinder. If the regulator does not fit, it may not be suitable for the gas you are using.
- Attach the appropriate hose connections to the flow control valve. Secure any tubing with clamps so that it will not whip around when pressure is turned on. Use suitable materials for connections; toxic and corrosive gases require connections made of special materials.
- Install a trap between the regulator and the reaction mixture to avoid backflow into the cylinder.
- To prevent a surge of pressure, turn the delivery pressure adjusting screw counterclockwise until it turns freely and then close the flow control valve.
- Slowly open the cylinder valve hand wheel until the cylinder pressure gauge reads the cylinder pressure.
- With the flow control valve closed, turn the delivery pressure screw clockwise until the delivery pressure gauge reads the desired pressure.
- Adjust the gas flow to the system by using the flow control valve or another flow control device between the regulator and the experiment.
- After an experiment is completed, turn the cylinder valve off first, and then allow gas to bleed from the regulator. When both gauges read “zero”, remove the regulator and replace the protective cap on the cylinder head.
- When the cylinder is empty, mark it as “Empty”, and store empty cylinders separate from full cylinders.
- Attach a “Full/In Use/Empty” tag to all of your cylinders, these tags are perforated and can be obtained from the gas cylinder vendor.
Precautions to follow:
- Use a regulator only with gas for which it is intended. The use of adaptors or homemade connectors has caused serious and even fatal accidents.
- Toxic gases should be purchased with a flow-limiting orifice.
- When using more than one gas, be sure to install one-way flow valves from each cylinder to prevent mixing. Otherwise accidental mixing can cause contamination of a cylinder.
- Do not attempt to put any gas into a commercial gas cylinder.
- Do not allow a cylinder to become completely empty. Leave at least 25 psi of residual gas to avoid contamination of the cylinder by reverse flow.
- Do not tamper with or use force on a cylinder valve.
16.4.4 Return of Cylinders16.4.4 Return of Cylinders
Disposal of cylinders and lecture bottles is expensive, especially if the contents are unknown.
- Make sure that all cylinders and lecture bottles are labeled and included in your chemical inventory. Before you place an order for a cylinder or lecture bottle, determine if the manufacturer will take back the cylinder or lecture bottle when it becomes empty.
- If at all possible, only order from manufacturers who will accept cylinders or lecture bottles for return.
16.4.5 Hazards of Specific Gases16.4.5 Hazards of Specific Gases
- Inert Gases - These can cause asphyxiation by displacing the air necessary for the support of life.
- Examples: Helium, Argon, Nitrogen
- Cryogens are capable of causing freezing burns, frostbite, and destruction of tissue.
- Cryogenic Liquids - Cryogenic liquids are extremely cold and their vapors can rapidly freeze human tissue.
- Boiling and splashing will occur when the cryogen contacts warm objects.
- Can cause common materials such as plastic and rubber to become brittle and fracture under stress.
- Liquid to gas expansion ratio: one volume of liquid nitrogen will vaporize and expand to about 700 times that volume, as a gas, and thus can build up tremendous pressures in a closed system. Therefore, dispensing areas need to be well ventilated. Avoid storing cryogenics in cold rooms, environmental chambers, and other areas with poor ventilation. If necessary, install an oxygen monitor/oxygen deficiency alarm and/or toxic gas monitor before working with these materials in confined areas.
- Oxidizers- Oxidizers vigorously accelerate combustion; therefore keep away from all flammable and organic materials. Greasy and oily materials should never be stored around oxygen. Oil or grease should never be applied to fittings or connectors.
- Examples: Oxygen, Chlorine
- Flammable Gases - Flammable gases are easily ignited by heat, sparks, or flames, and may form explosive mixtures with air. Vapors from liquefied gas often are heavier than air, and may spread along the ground and travel to a source of ignition and result in a flashback fire.
- Examples: Methane, Propane, Hydrogen, Acetylene, flammable gas mixtures.
- Flammable gases present serious fire and explosion hazards.
- Do not store near open flames or other sources of ignition.
- Cylinders containing Acetylene should never be stored on their side.
- Corrosive Gases- Corrosive gases readily attack the skin, mucous membranes, and eyes. Some corrosive gases are also toxic.
- Examples: Chlorine, Hydrogen Chloride, Ammonia
- There can be an accelerated corrosion of materials in the presence of moisture.
- Due to the corrosive nature of the gases, corrosive cylinders should only be kept on hand for 6 months (up to one year maximum). Only order the smallest size needed for your experiments
- Poison Gases- Poison gases are extremely toxic and present a serious hazard to laboratory staff.
- Examples: Arsine, Phosphine, Phosgene
- Poisonous gases require special ventilation systems and equipment and must only be used by properly trained experts. There are also special building code regulations that must be followed with regard to quantities kept on hand and storage.
- The purchase and use of poisonous gases require prior approval from EHS. Contact the Chemical Hygiene Officer at 607-255-8200.
16.4.6 Gas Generators16.4.6 Gas Generators
Gas Generators for Nitrogen or Hydrogen Gas
Gas generators are an alternative to having compressed gas cylinders in the laboratory. These provide high purity nitrogen or hydrogen and can be connected to lab equipment often without the New York State code implications due to the small quantities that are being stored.
Hydrogen Gas Generators:
- Produce gas on demand via electrolysis of water:
- Store up to 100 ml of gas:
- Require 15 megohm quality deionized water;
- Outlet pressures (psig) and flow rate requirements must be determined for installation.
Nitrogen Gas Generator:
- Produce gas via filtration from the air:
- This is a continuous supply;
- Have varying delivery flow rates. Typically maximum flows are 35 to 70 Liters/minute;
- Operate at maximum pressures of 116 psi (8 bar).
At flow rate requirements above 15 psi (1.05 bar), the Authority Having Jurisdiction may require gas detection. At lower flow rates they may waive this requirement. An engineering review is necessary. These generators do add to the heat load of the room in which they are located.
16.5 Battery Charging16.5 Battery Charging
Lead acid batteries contain corrosive liquids and also generate Hydrogen gas during charging which poses an explosion hazard. The following guidelines should be followed for battery charging areas:
- A “No smoking” sign should be posted.
- Before working, remove all jewelry from hands and arms and any dangling jewelry to prevent accidental contact with battery connections (this can cause sparks which can ignite vapors).
- Always wear appropriate PPE such as rubber or synthetic aprons, splash goggles (ideally in combination with a face shield), and thick Neoprene, Viton, or Butyl gloves.
- A plumbed emergency eyewash station must be readily available near the station (please note, hand held eyewash bottles do not meet this criteria.)
- A class B rated fire extinguisher needs to be readily available. If none is available, contact EHS at 607-255-8200.
- Ensure there is adequate ventilation available to prevent the buildup of potentially flammable and explosive gases.
- Keep all ignition sources away from the area.
- Stand clear of batteries while charging.
- Keep vent caps tight and level.
- Only use the appropriate equipment for charging.
- Store unused batteries in secondary containment to prevent spills.
- Have an acid spill kit available. The waste from a spill may contain lead and neutralized wastes may be toxic. Contact EHS at 607-255-8200 for hazardous waste disposal.
- Properly dispose of your used batteries.
16.6 Heat and Heating Devices16.6 Heat and Heating Devices
Heat hazards within laboratories can occur from a number of sources; however, there are some simple guidelines that can be followed to prevent heat related injuries. These guidelines include:
- Heating devices should be set up on a sturdy fixture and away from any ignitable materials (such as flammable solvents, paper products and other combustibles). Do not leave open flames (from Bunsen burners) unattended.
- Heating devices should not be installed near drench showers or other water spraying apparatus due to electrical shock concerns and potential splattering of hot water.
- Heating devices should have a backup power cutoff or temperature controllers to prevent overheating. If a backup controller is used, an alarm should notify the user that the main controller has failed.
- Provisions should be included in processes to make sure reaction temperatures do not cause violent reactions and a means to cool the dangerous reactions should be available.
- Post signs to warn people of the heat hazard to prevent burns.
When using ovens, the follow additional guidelines should be followed:
- Heat generated should be adequately removed from the area.
- If toxic, flammable, or otherwise hazardous chemicals are evolved from the oven, then only use ovens with a single pass through design where air is ventilated out of the lab and the exhausted air is not allowed to come into contact with electrical components or heating elements.
- Heating flammables should only be done with a heating mantle or steam bath.
When using heating baths, these additional guidelines should be followed:
- Heating baths should be durable and set up with firm support.
- Since combustible liquids are often used in heat baths, the thermostat should be set so the temperature never rises above the flash point of the liquid. Check the SDS for the chemical to determine the flashpoint. Compare that flashpoint with the expected temperature of the reaction to gauge risk of starting a fire.
16.6.1 Heat Stress16.6.1 Heat Stress
Another form of heat hazard occurs when working in a high heat area. Under certain conditions, your body might have trouble regulating its temperature. If your body cannot regulate its temperature, it overheats and suffers some degree of heat stress. This can occur very suddenly and, if left unrecognized and untreated, can lead to very serious health affects.
Heat stress disorders range from mild disorders such as fainting, cramps, or prickly heat to more dangerous disorders such as heat exhaustion or heat stroke. Symptoms of mild to moderate heat stress can include: sweating, clammy skin, fatigue, decreased strength, loss of coordination and muscle control, dizziness, nausea, and irritability. You should move the victim to a cool place and give plenty of fluids. Place cool compresses on forehead, neck, and under their armpits.
Heat stroke is a medical emergency. It can cause permanent damage to the brain and vital organs, or even death. Heat stroke can occur suddenly, with little warning. Symptoms of heat stroke may include: no sweating (in some cases victim may sweat profusely), high temperature (103? or more), red, hot, and dry skin, rapid and strong pulse, throbbing headache, dizziness, nausea, convulsions, delirious behavior, unconsciousness, or coma.
In the case of heat stroke, call 911 & get medical assistance ASAP! In the meantime, you should move the victim to a cool place, cool the person quickly by sponging with cool water and fanning, and offer a conscious person 1/2 glass of water every 15 minutes. There are a number of factors that affect your body’s temperature regulation:
- Radiant heat sources such as the sun or a furnace.
- Increased humidity causes decreased sweat evaporation.
- Decreased air movement causes decreased sweat evaporation.
- As ambient temperature rises, your body temperature rises and its ability to regulate decreases.
- You should be especially careful if:
- You just started a job involving physical work in a hot environment.
- You are ill, overweight, physically unfit, or on medication that can cause dehydration.
- You have been drinking alcohol.
- You have had a previous heat stress disorder.
In order to prevent heat stress, please follow these recommendations:
- Acclimatize your body to the heat. Gradually increase the time you spend in the heat. Most people acclimatize to warmer temperatures in 4-7 days. Acclimatization is lost when you have been away from the heat for one week or more. When you return, you must repeat the acclimatization process.
- Drink at least 4-8 ounces of fluid every 15-20 minutes to maintain proper balance during hot and/or humid environments. THIRST IS NOT A GOOD INDICATOR OF DEHYDRATION. Fluid intake must continue until well after thirst has been quenched.
- During prolonged heat exposure or heavy workload, a carbohydrate-electrolyte beverage is beneficial.
- Alternate work and rest cycles to prevent an overexposure to heat. Rest cycles should include relocation to a cooler environment.
- Perform the heaviest workloads in the cooler part of the day.
- There should be no alcohol consumption during periods of high heat exposure.
- Eat light, preferably cold meals. Fatty foods are harder to digest in hot weather.
16.7 Cold Traps16.7 Cold Traps
- Because many chemicals captured in cold traps are hazardous, care should be taken and appropriate protective equipment should be worn when handling these chemicals. Hazards include flammability, toxicity, and cryogenic temperatures, which can burn the skin.
- If liquid nitrogen is used, the chamber should be evacuated before charging the system with coolant. Since oxygen in air has a higher boiling point than nitrogen, liquid oxygen can be produced and cause an explosion hazard.
- Boiling and splashing generally occur when charging (cooling) a warm container, so stand clear and wear appropriate protective equipment. Items should be added slowly and in small amounts to minimize splash.
- A blue tint to liquid nitrogen indicates contamination with oxygen and represents an explosion hazard. Contaminated liquid nitrogen should be disposed of appropriately.
- If working under vacuum see the “reduced pressure” section.
- See “cryogenics” for safety advice when working with cryogenic materials.
16.8 Autoclaves16.8 Autoclaves
Autoclaves have the following potential hazards:
- Heat, steam, and pressure.
- Thermal burns from steam and hot liquids.
- Cuts from exploding glass.
Some general safety guidelines to follow when using autoclaves:
- All users should be given training in proper operating procedures for using the autoclave.
- Read the owner’s manual before using the autoclave for the first time.
- Operating instructions should be posted near the autoclave.
- Follow the manufacturer’s directions for loading the autoclave.
- Be sure to close and latch the autoclave door.
- Some kinds of bottles containing liquids can crack in the autoclave, or when they are removed from the autoclave. Use a tray to provide secondary containment in case of a spill, and add a little water to the tray to ensure even heating.
- Only fill bottles half way to allow for liquid expansion and loosen screw caps on bottles and tubes of liquid before autoclaving, to prevent them from shattering.
- Do not overload the autoclave compartment and allow for enough space between items for the steam to circulate.
- Be aware that liquids, especially in large quantities, can be superheated when the autoclave is opened. Jarring them may cause sudden boiling, and result in burns.
- At the end of the run, open the autoclave slowly: first open the door only a crack to let any steam escape slowly for several minutes, and then open all the way. Opening the door suddenly can scald a bare hand, arm, or face.
- Wait at least five minutes after opening the door before removing items.
- Large flasks or bottles of liquid removed immediately from the autoclave can cause serious burns by scalding if they break in your hands. Immediately transfer hot items with liquid to a cart; never carry in your hands.
- Wear appropriate PPE, including eye protection and insulating heat-resistant gloves.
16.9 Centrifuges16.9 Centrifuges
Some general safety guidelines to follow when using centrifuges:
- Be familiar with the operating procedures written by the manufacturer. Keep the operating manual near the unit for easy reference. If necessary contact the manufacturer to replace lost manuals.
- Handle, load, clean, and inspect rotors as recommended by the manufacturer.
- Pay careful attention to instructions on balancing samples -- tolerances for balancing are often very restricted. Check the condition of tubes and bottles. Make sure you have secured the lid to the rotor and the rotor to the centrifuge.
- Maintain a logbook of rotor use for each rotor, recording the speed and length of time for each use.
- To avoid catastrophic rotor failure, many types of rotors must be "de-rated" (limited to a maximum rotation speed that is less than the maximum rotation speed specified for the rotor when it is new) after a specified amount of use, and eventually taken out of service and discarded.
- Use only the types of rotors that are specifically approved for use in a given centrifuge unit.
- Maintain the centrifuge in good condition. Broken door latches and other problems should be repaired before using the centrifuge.
16.9.1 Centrifuge Rotor Care16.9.1 Centrifuge Rotor Care
Basic centrifuge rotor care includes:
- Keep the rotor clean and dry, to prevent corrosion.
- Remove adapters after use and inspect for corrosion.
- Store the rotor upside down, in a warm, dry place to prevent condensation in the tubes.
- Read and follow the recommendations in the manual regarding:
- Regular cleaning
- Routine inspection
- Regular polishing
- Lubricating O-rings
- Decontaminating the rotor after use with radioactive or biological materials
- Remove any rotor from use that has been dropped or shows any sign of defect, and report it to a manufacturer’s representative for inspection.
There is a description of an accident that occurred at Cornell and how to prevent centrifuge accidents on the Centrifuge Accident webpage.
16.10 Cryogenic Material Safety16.10 Cryogenic Material Safety
According to the Compressed Gas Association, a cryogenic fluid is a material that has a boiling point of less than -130°F (-90°C). Examples of cryogenic materials include the liquids nitrogen, argon, and helium, and solid carbon dioxide (dry ice). Hazards associated with cryogenic fluids include:
Extreme Low Temperature - These liquids and their boiled off vapors are extremely cold and can cause severe cold contact burns. They also make many materials brittle, such as the epoxy or phenolic resin that laboratory benchtops and sinks are made of.
Asphyxiation - When liquid and solid cryogenic materials vaporize they greatly expand, some by a factor of ~700:1. This displaces oxygen and creates an oxygen depletion hazard. This is especially dangerous when confined in poorly ventilated space like an elevator.
Oxygen makes up 20.9% of the air we breathe. The environment is considered to be oxygen deficient below 19.5%. Two breaths of air with no oxygen can be enough to render a person unconscious.
Typical background levels of carbon dioxide are about 400 ppm. Vaporization of even small quantities of Dry Ice in an unventilated area can exceed the permissible exposure limit of 5000 ppm.
Exposure to oxygen-deficient atmospheres produce dizziness, nausea, vomiting, loss of consciousness, and death. Such symptoms may occur in seconds without warning.
Death may result from errors in judgment, confusion, or loss of consciousness that prevents self-rescue. Working with cryogenic substances in confined spaces, such as walk-in coolers, can be especially hazardous. Where cryogenic materials are used, a hazard assessment is required to determine the potential for an oxygen-deficient condition. Controls such as ventilation and/or gas detection systems may be required to safeguard employees. Asphyxiation and chemical toxicity are hazards encountered when entering an area that has been used to store cryogenic liquids if proper ventilation/purging techniques are not employed.
Toxicity - Many of the commonly used cryogenic gases are considered to be of low toxicity, but still pose a hazard from asphyxiation. Check the properties of the gases you are using because some gases are toxic, for example, Carbon monoxide, Fluorine, and Nitrous oxide.
Flammability and Explosion - Fire or explosion may result from the evaporation and vapor buildup of flammable gases such as hydrogen, carbon monoxide, or methane. Liquid oxygen, while not itself a flammable gas, can combine with combustible materials and greatly accelerate combustion.
Oxygen clings to clothing and cloth items, and presents an acute fire hazard.
High Pressure - In cryogenic systems, high pressures are obtained by gas compression during refrigeration. Warm temperatures surrounding containers cause a constant expansion of the liquid as it turns back into gas. Sudden release through a rupture or break in a line may be violent. Over- pressurization of cryogenic equipment can occur due to improper venting and expansion during the phase change from liquid to gas. All cryogenic fluids produce large volumes of gas when they vaporize.
Materials and Construction Hazards - The selection of materials calls for consideration of the effects of low temperatures on the properties of those materials. Some materials become brittle at super low temperatures. Brittle materials fracture easily and can result in almost instantaneous material failure.
Low temperature equipment can also fail due to thermal stresses caused by differential thermal contraction of the materials. Over-pressurization of cryogenic equipment can occur due to the phase change from liquid to gas if not vented properly.
16.10.1 Cryogenic Safety Guidelines16.10.1 Cryogenic Safety Guidelines
Personnel who are responsible for any cryogenic equipment must conduct a safety review prior to the commencement of operation of the equipment. Supplementary safety reviews must follow any system modification to ensure that no potentially hazardous condition is overlooked or created, and that updated operational and safety procedures remain adequate.
Clothing and Personal Protective Equipment
Wear the appropriate clothing and PPE when working and transferring cryogenic materials.
- Safety glasses are a minimum to be worn during the transfer and normal handling of cryogenic fluids. Face shields must be worn over safety glasses if there is the potential for splashing in the face, such as filling dewars.
- Loose fitting, heavy leather or other insulating protective gloves must be worn when handling cryogenic fluids.
- Shirt sleeves should be rolled down and buttoned over glove cuffs, or equivalent protection, such as a lab coat, should be worn in order to prevent liquid from spraying or spilling inside the gloves. Do not wear short sleeves when working with cryogenic liquids.
- Long pants without cuffs and that don’t expose skin must be worn.
- Shoes that cover the feet are required. These must not allow cryogenic materials to come into contact with skin. Shoes must not allow a cryogenic liquid to become trapped in the material or soak in liquid in the event of a spill. Leather or other non-woven material is recommended.
Cryogenic fluids must be handled and stored only in containers and piping systems specifically designed to withstand the extremely cold temperatures, and in accordance with applicable standards, procedures, and proven safe practices, and be based on the specific cryogen.
Transfer operations involving open cryogenic containers such as dewars must be conducted slowly to minimize boiling and splashing of the cryogenic fluid. Transfer of cryogenic fluids from open containers must occur below chest level of the person pouring the liquid and on a steady surface.
Only conduct such operations in well-ventilated areas to prevent possible gas or vapor accumulation that may produce an oxygen-deficient atmosphere and lead to asphyxiation. If this is not possible, an oxygen monitor must be installed.
Small spaces, environmental chambers, and cold rooms often do not have sufficient exhaust ventilation to support storage and use of cryogenic materials. Contact EHS with any questions and to conduct a risk assessment.
All cryogenic systems, including piping, must be equipped with pressure relief devices to prevent excessive pressure build-up. If a container vents continuously, shows signs of blockage by not venting at all, or there is frost buildup on the outside, which indicates that there is loss of vacuum, do the following:
Do not attempt to remove a blockage;
Move the vessel to a remote location or notify others of the problem;
Contact the supplier for assistance with the vessel.
- The caps of liquid nitrogen dewars are designed to fit snugly to contain the liquid nitrogen, but also allow the periodic venting that will occur to prevent over pressurization of the vessel. Do not ever attempt to seal the caps of liquid nitrogen dewars. Doing so can present a significant hazard of over pressurization that could rupture the container and cause splashes of liquid nitrogen. Depending on the amount of liquid nitrogen that may be spilled, this could cause an oxygen deficient atmosphere within a laboratory due to the sudden release and vaporization of the cryogenic liquid.
- In the event of a spill, oxygen deficiency or a flammable atmosphere will exist beyond any visible fog cloud.
Emergency treatment if skin or eyes come into contact with cryogenic liquid or vapor is as follows:
- If the cryogenic fluid comes in contact with the skin or eyes, flush the affected area with generous quantities of cold water. Never use dry heat. Splashes on bare skin cause a stinging sensation, but in general are not harmful.
- If clothing becomes soaked with liquid, it should be removed as quickly as possible and the affected area should be flooded with water as above.
- Where clothing has frozen to the underlying skin, cold water should be poured on the area, but no attempt should be made to remove the clothing until it is completely free.
- If inhalation of the cold vapors has occurred, move the person to warm, fresh air. The person may be suffering from frostbite tissue in their throat and lungs, and also asphyxia.
- Do NOT rub frostbitten skin as tissue damage may occur. Place in a warm bath that is not above 105°F (40°C).
- For emergencies: Call 607-255-1111 from a cell phone or 911 from a campus phone.
- Complete an Injury/Illness Report
16.10.2 Cryogenic Chemical Specific Information16.10.2 Cryogenic Chemical Specific Information
Liquid helium must be transferred via helium pressurization in properly designed transfer lines. A major safety hazard may occur if liquid helium comes in contact with air. Air solidifies in contact with liquid helium, and precautions must be taken when transferring liquid helium from one vessel to another or when venting. Over-pressurization and rupture of the container may result. All liquid helium containers must be equipped with a pressure-relief device. The latent heat of vaporization of liquid helium is extremely low (20.5 J/gm); therefore, small heat leaks can cause rapid pressure rises.
Since the boiling point of liquid nitrogen is below that of liquid oxygen, it is possible for oxygen to condense on any surface cooled by liquid nitrogen. If the system is subsequently closed and the liquid nitrogen removed, the evaporation of the condensed oxygen may over-pressurize the equipment or cause a chemical explosion if exposed to combustible materials, e.g., the oil in a rotary vacuum pump. In addition, if the mixture is exposed to radiation ozone is formed, which freezes into ice and is very unstable. An explosion can result if this ice is disturbed. For this reason, air should not be admitted to enclosed equipment that is below the boiling point of oxygen unless specifically required by a written procedure.
Any transfer operations involving open containers such as wide-mouth dewars must be conducted slowly to minimize boiling and splashing of liquid nitrogen. The transfer of liquid nitrogen from open containers must occur below chest level of the person pouring the liquid.
If liquid nitrogen or helium traps are used to remove condensable gas impurities from a vacuum system that may be closed off by valves, the condensed gases will be released when the trap warms up.
Adequate means for relieving resultant build-up of pressure must be provided.
Any proposal for the use of liquid hydrogen must obtain prior approval and undergo an EHS risk assessment and engineering code review.
- Because of its wide flammability range and ease of ignition, special safety measures must be invoked when using liquid hydrogen.
- Liquid hydrogen must be transferred by helium pressurization in properly designed transfer lines to avoid contact with air. Properly constructed and certified vacuum insulated transfer lines should be used.
- Rooms where flammable gas is used must be positively pressurized.
- Only trained personnel familiar with liquid hydrogen properties, equipment, and operating procedures are permitted to perform transfer operations. Transfer lines in liquid hydrogen service must be purged with helium or gaseous hydrogen, with proper precautions, before using.
The safety philosophy in the use of liquid hydrogen can be summarized as the following:
- Isolation of the experiment. Provision of adequate ventilation.
- Exclusion of ignition sources plus system grounding/bonding to prevent static charge build-up. Containment in helium purged vessels.
- Efficient monitoring for hydrogen leakage.
- Limiting the amount of hydrogen cryo-pumped in the vacuum system.
Any proposal to use liquid oxygen must obtain prior approval and undergo an EHS risk assessment and engineering code review.
- Liquid oxygen does not burn; however, it greatly accelerates fire. Adequate ventilation to avoid oxygen enrichment of the air is necessary.
- A room with combustible materials, oils and grease, electrical equipment, an ignition source, or flammable liquids and vapors should be avoided.
- OSHA defines an oxygen enriched atmosphere as being more than 22% oxygen. Oxygen enriched atmospheres present a significant fire and explosion risk.
- In the event of saturation of clothing with liquid oxygen, stay away from ignition sources for at least 30 minutes or change clothing.
16.10.3 Handling Cryogenic Materials on Elevators16.10.3 Handling Cryogenic Materials on Elevators
There is typically little or no fresh air supplied inside of elevators. Cryogenic materials displace air. To avoid a situation in which there is insufficient breathable air, the following are expected in Cornell buildings:
- Quantities up to 5 liters of a cryogenic material are acceptable to travel in an elevator accompanied by people.
- Quantities greater than 5 liters of cryogenic material must be transported on an elevator unaccompanied as it travels between floors. Do NOT transport a pressurized container in an elevator with any person/s in the elevator car.
- Transport in a freight elevator, if possible.
- Post a sign on the vessel in the elevator reading “DO NOT ENTER – CRYOGEN ASPHYXIATION HAZARD” to warn potential passengers.
- Have another person available on the receiving floor to take the container off the elevator at its destination.
- Beware of tipping the vessel while moving on or off the elevator. This is especially important of uncapped dewars of liquid nitrogen.
- Wear insulated cryogen safety gloves, safety goggles, long shirt, and long pants without cuffs. Wear footwear that will not soak in the liquid in case of a spill (e.g. leather instead of woven mesh).
- In case of a spill of any quantity on an elevator:
- Press the button to stop at the nearest floor;
- Block the elevator door open so that it doesn't move to the next floor;
- Call 607-255-1111 from a cell phone or 911 from a campus phone.
16.11 Extractions and Distillations16.11 Extractions and Distillations
- Do not attempt to extract a solution until it is cooler than the boiling point of the extractant due to the risk of overpressurization, which could cause the vessel to burst.
- When a volatile solvent is used, the solution should be swirled and vented repeatedly to reduce pressure before separation.
- When opening the stopcock, your hand should keep the plug firmly in place.
- The stopcock should be lubricated.
- Vent funnels away from ignition sources and people, preferably into a hood.
- Keep volumes small to reduce the risk of overpressure and if large volumes are needed, break them up into smaller batches.
- Avoid bumping (sudden boiling) since the force can break apart the apparatus and result in splashes. Bumping can be avoided by even heating, such as using a heat mantle. Also, stirring can prevent bumping. Boiling stones can be used only if the process is at atmospheric pressure.
- Do not add solid items such as boiling stones to liquid that is near boiling since it may result in the liquid boiling over spontaneously.
- Organic compounds should never be allowed to boil to dryness unless they are known to be free of peroxides, which can result in an explosion hazard.
Reduced pressure distillation
- Do not overheat the liquid. Superheating can result in decomposition and uncontrolled reactions.
- Superheating and bumping often occur at reduced pressures so it is especially important to abide by the previous point on bumping and to ensure even, controlled heating. Inserting a nitrogen bleed tube may help alleviate this issue.
- Evacuate the assembly gradually to minimize bumping.
- Allow the system to cool and then slowly bleed in air. Air can cause an explosion in a hot system (pure nitrogen is preferable to air for cooling).
- See “reduced pressure” for vacuum conditions.
16.12 Glass Under Vacuum16.12 Glass Under Vacuum
Some general guidelines for glass under vacuum include:
- Inspect glassware that will be used for reduced pressure to make sure there are no defects such as chips or cracks that may compromise its integrity.
- Only glassware that is approved for low pressure should be used. Never use a flat bottom flask (unless it is a heavy walled filter flask) or other thin walled flask that are not appropriate to handle low pressure.
- Use a shield between the user and any glass under vacuum or wrap the glass with tape to contain any glass in the event of an implosion.
- Cold traps should be used to prevent pump oil from being contaminated which can create a hazardous waste.
- Pump exhaust should be vented into a hood when possible.
- Ensure all belts and other moving parts are properly guarded.
- Whenever working on or servicing vacuum pumps, be sure to follow appropriate lock-out procedures.
16.13 Glassware Washing16.13 Glassware Washing
In most cases laboratory glassware can be cleaned effectively by using detergents and water. In some cases it may be necessary to use strong chemicals for cleaning glassware. Strong acids should be avoided unless necessary. In particular, Chromic acid should not be used due to its toxicity and disposal concerns. One product that may be substituted for Chromic acid is “Nochromix Reagent”. The Fisher catalog describes this material as: “Nochromix Reagent. Inorganic oxidizer chemically cleans glassware. Contains no metal ions. Rinses freely—leaving no metal residue, making this product valuable for trace analysis, enzymology, and tissue culture work. (Mix with sulfuric acid).” Unused Nochromix Reagent can be neutralized to a pH between 5.5 and 9.5 and drain disposed. Acid/base baths should have appropriate labeling and secondary containment. Additionally a Standard Operating Procedure (SOP), proper personal protective equipment (PPE), and spill materials should be available. Proper disposal for spent acid/base bath contents is neutralization and drain disposal.
When handling glassware, check for cracks and chips before washing, autoclaving or using it. Dispose of chipped and broken glassware immediately in an approved collection unit. DO NOT put broken glassware in the regular trash. Handle glassware with care – avoid impacts, scratches or intense heating of glassware. Make sure you use the appropriate labware for the procedures and chemicals.
Use care when inserting glass tubing into stoppers:
- Use glass tubing that has been fire-polished
- Lubricate the glass
- Protect your hands with heavy gloves
If your department/building has a glass washing service there are certain protocols that must be followed before sending the glassware to be washed. It is the responsibility of the lab to empty and rinse all glassware before it leaves the lab. Although the contents may not be hazardous, the washroom support staff cannot be certain of the appropriate PPE to wear, disposal regulations or possible incompatibilities with items received from other researchers. Be aware that labeling for lab personnel is not sufficient for areas outside the lab as per the OSHA Hazard Communication Standard. It is the responsibility of the glassware washing staff to reject or return glassware that is not acceptable due to breakage or containing chemicals. For this reason, glassware should be labeled with the name of the person who is responsible for it.
16.14 General Equipment Set Up16.14 General Equipment Set Up
The following recommended laboratory techniques for general equipment set up was taken from the American Chemical Society’s booklet – Safety in Academic Chemistry Laboratories.
16.14.1 Glassware and Plasticware16.14.1 Glassware and Plasticware
- Borosilicate glassware (i.e. pyrex) is recommended for all lab glassware, except for special experiments using UV or other light sources. Soft glass should only be used for things such as reagent bottles, measuring equipment, stirring rods and tubing.
- Any glass equipment being evacuated, such as suction flasks, should be specially designed with heavy walls. Dewar flasks and large vacuum vessels should be taped or guarded in case of flying glass from an implosion. Household thermos bottles have thin walls and are not acceptable substitutes for lab Dewar flasks.
- Glass containers containing hazardous chemicals should be transported in rubber bottle carriers or buckets to protect them from breakage and contain any spills or leaks. It is recommended to transport plastic containers this way as well since they also can break or leak.
16.14.2 Preparation of Glass Tubing and Stoppers16.14.2 Preparation of Glass Tubing and Stoppers
- To cut glass tubing:
- Hold the tube against a firm support and make one firm quick stroke with a sharp triangular file or glass cutter to score the glass long enough to extend approximately one third around the circumference.
- Cover the tubing with cloth and hold the tubing in both hands away from the body. Place thumbs on the tubing opposite the nick 2 to 3 cm and extended toward each other.
- Push out on the tubing with the thumbs as you pull the sections apart, but do not deliberately bend the glass with the hands. If the tubing does not break, re-score the tube in the same place and try again. Be careful to not contact anyone nearby with your motion or with long pieces of tubing.
- All glass tubing, including stir rods, should be fire polished before use. Unpolished tubing can cut skin as well as inhibit insertion into stoppers. After polishing or bending glass, give ample time for it to cool before grasping it.
- When drilling a stopper:
- Use only a sharp borer one size smaller than that which will just slip over the tube to be inserted. For rubber stoppers, lubricate with water or glycerol. Holes should be bored by slicing through the stopper, twisting with moderate forward pressure, grasping the stopper only with the fingers, and keeping the hand away from the back of the stopper.
- Keep the index finger of the drilling hand against the barrel of the borer and close to the stopper to stop the borer when it breaks through. Preferably, drill only part way through and then finish by drilling from the opposite side.
- Discard a stopper if a hole is irregular or does not fit the inserted tube snugly, if it is cracked, or if it leaks.
- Corks should have been previously softened by rolling and kneading. Rubber or cork stoppers should fit into a joint so that one-third to one–half of the stopper is inserted.
- When available, glassware with ground joints is preferable. Glass stoppers and joints should be clean, dry and lightly lubricated.
16.14.3 Insertion of Glass Tubes or Rods into Stoppers16.14.3 Insertion of Glass Tubes or Rods into Stoppers
The following practices will help prevent accidents:
- Make sure the diameter of the tube or rod is compatible with the diameter of the hose or stopper.
- If not already fire polished, fire polish the end of the glass to be inserted; let it cool.
- Lubricate the glass. Water may be sufficient, but glycerol is a better lubricant.
- Wear heavy gloves or wrap layers of cloth around the glass and protect the other hand by holding the hose or stopper with a layered cloth pad.
- Hold the glass not more than 5 cm from the end to be inserted.
- Insert the glass with a slight twisting motion, avoiding too much pressure and torque.
- When helpful, use a cork borer as a sleeve for insertion of glass tubes.
- If appropriate, substitute a piece of metal tubing for glass tubing.
- Remove stuck tubes by slitting the hose or stopper with a sharp knife.
16.14.4 Assembling Apparatus16.14.4 Assembling Apparatus
Following these recommendations will help make apparatus assembly easier and equipment safer:
- Keep your work space free of clutter.
- Set up clean, dry apparatus, firmly clamped and well back from the edge of the lab bench making adequate space between your apparatus and others work. Choose sizes that can properly accommodate the operation to be performed. As a rule, leave about 20% free space around your work.
- Use only equipment that is free from flaws such as cracks, chips, frayed wire, and obvious defects. Glassware can be examined in polarized light for strains. Even the smallest crack or chip can render glassware unusable. Cracked or chipped glassware should be repaired or discarded.
- A properly placed pan under a reaction vessel or container will act as secondary containment to confine spilled liquids in the event of glass breakage.
- When working with flammable gases or liquids, do not allow burners or other ignition sources in the vicinity. Use appropriate traps, condensers, or scrubbers to minimize release of material to the environment. If a hot plate is used, ensure the temperatures of all exposed surfaces are less than the autoignition temperature of the chemicals likely to be released and that the temperature control device and the stirring / ventilation motor (if present) do not spark.
- Whenever possible, use controlled electrical heaters or steam in place of gas burners.
- Addition and separatory funnels should be properly supported and oriented so that the stopcock will not be loosened by gravity. A retainer ring should be used on the stopcock plug. Glass stopcocks should be freshly lubricated. Teflon stopcocks should not be lubricated.
- Condensers should be properly supported with securely positioned clamps and the attached water hoses secured with wire or clamps.
- Stirrer motors and vessels should be secured to maintain proper alignment. Magnetic stirring is preferable. Only non-sparking motors should be used in chemical laboratories. Air motors may be an option.
- Apparatus attached to a ring stand should be positioned so that the center of gravity of the system is over the base and not to one side. There should be adequate provision for removing burners or baths quickly. Standards bearing heavy loads should be firmly attached to the bench top. Equipment racks should be securely anchored at the top and bottom.
- Apparatus, equipment, or chemical bottles should not be placed on the floor. If necessary, keep these items under tables and out of aisleways to prevent creating a tripping hazard.
- Never heat a closed container. Provide a vent as part of the apparatus for chemicals that are to be heated. Prior to heating a liquid, place boiling stones in unstirred vessels (except test tubes). If a burner is used, distribute the heat with a ceramic-centered wire gauze. Use the thermometer with its bulb in the boiling liquid if there is the possibility of a dangerous exothermic decomposition as in some distillations. This will provide a warning and may allow time to remove the heat and apply external cooling. The setup should allow for fast removal of heat.
- Whenever hazardous gases or fumes are likely to be evolved, an appropriate gas trap should be used and the operation confined to a fume hood.
- Fume hoods are recommended for all operations in which toxic or flammable vapors are evolved as is the case with many distillations. Most vapors have a density greater than air and will settle on a bench top or floor where they may diffuse to a distant burner or ignition source. These vapors will roll out over astonishingly long distances and, if flammable, an ignition can cause a flash back to the source of vapors. Once diluted with significant amounts of air, vapors move in air essentially as air itself.
- Use a hood when working with a system under reduced pressure (which may implode). Close the sash to provide a shield. If a hood is not available, use a standing shield. Shields that can be knocked over must be stabilized with weights or fasteners. Standing shields are preferably secured near the top. Proper eye and face protection must be worn even when using safety shields or fume hoods.
16.14.5 Mercury Containing Equipment16.14.5 Mercury Containing Equipment
Elemental Mercury (Hg) or liquid Mercury is commonly seen in thermometers, barometers, diffusion pumps, sphygmomanometers, thermostats, high intensity microscope bulbs, fluorescent bulbs, UV lamps, batteries, Coulter Counter, boilers, ovens, welding machines, etc.
Most of these items can be substituted with equipment without Mercury, thus greatly decreasing the hazard potential. Larger laboratory equipment may be more difficult to identify as “Mercury containing” due to the fact that mercury can be hidden inside inner components such as switches or gauges.
The concerns surrounding mercury containing equipment are:
- It is difficult to identify exposures or cross-contamination due to Mercury leaks or spills.
- The amount of Mercury used is usually much greater than the Department of Environmental Conservation (DEC) reportable quantities for releases to the environment.
- People may be unaware of the Mercury and thus may not be properly trained for use, maintenance, spills, transport or disposal or may not use the appropriate engineering controls or Personal Protective Equipment (PPE).
- There is legal liability if human health and the environment are not properly protected.
To minimize the potential for Mercury spills and possible exposures, laboratory personnel is strongly encouraged to follow these recommendations:
- Identify and label “Mercury Containing Equipment”.
- Write a Standard Operating Procedure (SOP).
- Train personnel on proper use, maintenance, transport and disposal.
- Conduct periodic inspections of equipment to ensure no leaks or spills have occurred.
- Consider replacing Mercury with electronic or other replacement components.
- Have available proper PPE such as nitrile gloves.
- Use secondary containment, such as trays as a precaution for spills.
- Plan for emergency such as a spill or release of mercury.
- Decontaminate and remove Mercury before long-term storage, transport or disposal.
- For new equipment purchases, please attempt to procure instruments with no or little Mercury
16.15 Ergonomics16.15 Ergonomics
Many lab tasks such as looking through microscopes, working in exhaust hoods, pipetting, and continuously looking down for bench tasks require both significant repetitive movements and sustained awkward posturing. Often there is no leg room when sitting at counters or hoods, which causes more leaning and reaching. Although the essential job tasks probably cannot change, you can develop important personal strategies that can improve comfort and health. There may also be equipment changes you can make.
The section below outlines some steps you can take to reduce your risk for injury from this demanding work. Links to product ideas and additional related information are provided. Product links do not imply endorsement. Consider a free ergonomic evaluation of your specific environment (Cornell Musculoskeletal Injury Prevention Program)—additional information below) before purchasing equipment.
- Take the time to adjust the seat depth and chair back height and tilt in order to maximize individual back support. Consider a slightly reclined position to promote better support.
- Try using chairs “backward”, supporting the torso when leaning forward to do bench/hood/microscope work, as a means for changing positions throughout the day.
- Make sure the feet reach the floor, foot ring or separate footrest comfortably. The stabilization of both feet makes it easier to sit back in a supported manner. Some lab chairs have adjustable foot rings—consider this feature when buying new chairs. For lower surfaces use office-style footrests. NeXtep are adjustable rests that attach to the cylinder of lab stools. Another style of freestanding rest with extended height adjustment is by Safco or similar.
- Seat height—be sure lab chairs have adequate height adjustment. Extended cylinder heights (32 inch) may provide additional adjustment that will help employees comfortably reach/perform work at counter height.
- Pull your torso close to the work surface and then sit back. This technique will help avoid ‘perching’ on the edge of the chair.
- Select benches where there is leg room under the surface.
Standing all day for bench work, particularly on concrete/tile flooring, is difficult. The body requires time to recover from these demands, even within a given shift.Recommendations to minimize risks from extended standing include:
- Microbreaks--allow time (as little as 30 seconds - 1 minute every 20 minute) and a chair/stool so spinal structures and joints can recover from extended standing.
- Consider anti-fatigue matting in areas where practical.
- Proper footwear is important and using a foam/gel insole can also reduce fatigue. Remember, they need to be replaced before they appear worn out.
- Provide a footrest so you can elevate one foot, then the other. This will reduce static fatigue. If safe/appropriate, try opening cabinets to create a footrest.
- Be cognizant of neutral postures while working. Adjust the chair or microscope as needed to maintain an upright head position. Elevate, tilt or move the microscope closer to the edge of the counter to avoid bending your neck.
- Avoid leaning on the hard edges on the table - consider padding the front lip of microscope table (AliEdge or similar) or using forearm pads. A simple, versatile solution is a variety of foam pads, like Wedge-Ease. Be sure these supports do not cause awkward wrist postures when focusing/adjusting the stage.
- Keep scopes repaired and clean.
- Spread microscope work throughout the day and between several people, if possible.
- Observe seating adjustment and support techniques.
Below are some general guidelines to reduce the physical impact of pipetting.
- Sit or stand close to your work at bench. If safe/appropriate, try opening cabinets to create legroom.
- Work at appropriate heights to minimize twisting of the neck and torso. Elevate your chair rather than reaching up to pipette.
- Alternate or use both hands to pipette.
- Select a lightweight pipette sized for your hand. Hold the pipette with a relaxed grip and use minimal pressure while pipetting.
- Avoid standing or sitting for long periods. Alternating between sitting and standing provides relief and recovery time for fatigued body structures. Additional resources can be found at UCLA, UC Berkeley
- Observe seating recommendations to promote supported postures.
- Position work supplies as close as possible in order to avoid awkward leaning/reaching while working. Consider turntables to rotate materials closer to the user. Be sure that only essential materials are in the hood to avoid unnecessary reaching around clutter.
- Consider lower-profile sample holders, solution container, waste receptacles to prevent awkward bending of wrist, neck and shoulders. Reduced repetitive movement also means increased efficiency.
Additional resources can be found at UCLA.
- Gloves — Wear slightly snug gloves to reduce forces on hands and improve accuracy during fine manipulation. Wearing loose gloves during pipetting and other tasks makes manipulating small materials more forceful and difficult.
- Rotate tasks throughout the work day and among several people, whenever possible. Take frequent small rest breaks (1-2 minute in duration) every 20 minutes. Every 45-60 minutes, get up to stretch and move.
- Take vision breaks during intensive computer and fine visual work. Every 20 minutes, close the eyes or focus on something in the distance.
Cornell Musculoskeletal Injury Prevention Program (MIPP). The MIPP provides ergonomics assessment, training and planning services to the Cornell Community. All Cornell employees are eligible for services with the approval of their supervisor or Human Resources representative. Benefit Services demonstrates its commitment to employee health by offering the MIPP at no charge to Cornell employees and departments. Remember, you do not have to be uncomfortable or injured to benefit from MIPP services. Ergonomics is most effective when services are utilized for prevention. For more information, contact the Lead Ergonomics Consultant at Cornell Musculoskeletal Injury Prevention Program.
Early treatment of discomfort/injury and the continuous development a healthier lifestyle are critical to remaining healthy and productive at work. Take advantage these valuable resources: Cornell Physical Therapy and Cornell Wellness.
Appendix B - Contact ListAppendix B - Contact List
A list of Subject Matter Experts at Cornell University: EHS Subject Matter Expert List
Appendix C - Compiled Lab Safety ResponsibilitiesAppendix C - Compiled Lab Safety Responsibilities
- It is the responsibility of Principal Investigators and laboratory supervisors to ensure that personnel working in laboratories under their control are familiar with the contents and location of the Chemical Hygiene Plan, including any lab specific standard operating procedures and any department or college level laboratory safety manuals, policies, and procedures. (Section 1.1)
- It is the responsibility of the Principal Investigator and individual supervisors (and individuals working under their supervision) to be in compliance with the components of the University Chemical Hygiene Plan, the University Health and Safety Policy, and any other department or University specific policies. (Section 1.2)
- It is the responsibility of laboratory personnel to immediately report malfunctioning protective equipment, such as fume hoods, or mechanical problems to their Building Coordinator as soon as any malfunctions are discovered. (Section 2.1)
- Principal Investigators, laboratory supervisors, departments and colleges are free to set policies that establish minimum PPE requirements for personnel working in and entering their laboratories. Be sure to check with your DSR to see if there are any department or college specific requirements for PPE. (Section 3.1)
- It is the responsibility of the Principal Investigator or laboratory supervisor to ensure laboratory staff have received the appropriate training on the selection and use of proper PPE, that proper PPE is available and in good condition, and laboratory personnel use proper PPE when working in laboratories under their supervision. (Section 3.2)
- It is the responsibility of Principal Investigators and laboratory supervisors to make use of eye protection a mandatory requirement for all laboratory personnel, including visitors, working in or entering laboratories under their control. (Section 3.3)
- EHS strongly recommends that Principal Investigators and laboratory supervisors discourage the wearing of shorts and skirts in laboratories using hazardous materials (chemical, biological, and radiological) by laboratory personnel, including visitors, working in or entering laboratories under their supervision. (Section 3.5)
- EHS strongly encourages Principal Investigators and laboratory supervisors to require the use of closed toed shoes for all laboratory personnel, including visitors, working in or entering laboratories and laboratory support areas under their supervision. (Section 3.8)
- It is the responsibility of the Principal Investigator and laboratory supervisor to ensure that personnel working in laboratories under their supervision are informed and follow laboratory specific, departmental, and campus wide policies and procedures related to laboratory safety – such as the guidelines and requirements covered in this Laboratory Safety Manual. (Section 4.0)
- It is the responsibility of the Principal Investigator and laboratory supervisor to ensure written SOPs incorporating health and safety considerations are developed for work involving the use of hazardous chemicals in laboratories under their supervision and that PPE and engineering controls are adequate to prevent overexposure. In addition, Principal Investigators and laboratory supervisors must ensure that personnel working in laboratories under their supervision have been trained on those SOPs. (Section 4.1)
- It is the responsibility of Principal Investigators and laboratory supervisors to ensure laboratories under their supervision are maintained in a clean and orderly manner and personnel working in the lab practice good housekeeping. (Section 4.4)
- It is the responsibility of Principal Investigators and laboratory supervisors to ensure procedures for working alone are developed and followed by personnel working in laboratories under their supervision. (Section 4.7)
- It is the responsibility of Principal Investigators and laboratory supervisors to ensure procedures for unattended operations are developed and followed by personnel working in laboratories under their supervision. (Section 4.9)
- It is the responsibility of the Department Chairperson, Principal Investigators, and laboratory supervisors to restrict access of visitors and children to areas under their supervision when potential health and physical hazards exist. (Section 4.10.1)
- It is the responsibility of the Principal Investigator and individual supervisors to ensure research areas under their supervision have been registered using the online HASP program. (Section 4.19)
- It is the responsibility of laboratory personnel to activate (flush) emergency showers and eyewash units on a regular basis. (Section 5.5.1)
- It is the responsibility of the Principal Investigator and laboratory supervisor to ensure all injuries are reported to University officials through the use of the Cornell University injury/illness reporting system. (Section 5.6)
- It is the responsibility of Principal Investigators and laboratory supervisors to ensure personnel working in laboratories under their supervision have been provided with the proper training, have received information about the hazards in the laboratory they may encounter, and have been informed about ways they can protect themselves. (Section 6.0)
- It is the responsibility of the Principal Investigator and laboratory supervisor to ensure that staff and students under their supervision are provided with adequate training and information specific to the hazards found within their laboratories. (Section 7.2.1)
- It is the responsibility of Principal Investigators and laboratory supervisors to ensure that staff and students working in laboratories under their supervision have obtained required health and safety training and have access to SDSs (and other sources of information) for all hazardous chemicals used in laboratories under their supervision. (Section 7.3)
- While EHS can provide assistance in identifying circumstances when there should be prior approval before implementation of a particular laboratory operation, the ultimate responsibility of establishing prior approval procedures lies with the Principal Investigator or laboratory supervisor. (Section 9.5)
- It is the responsibility of the Principal Investigator and laboratory supervisor to ensure that personnel working in laboratories under their supervision are familiar with and follow hazardous chemical waste container requirements and have attended EHS Chemical Waste Disposal training. (Section 10.1)
- It is the responsibility of the Principal Investigator or laboratory supervisor to ensure any employee working under their supervision who ships or prepare shipments of hazardous materials have received the proper training. (Section 11.0)
- The responsibility for ensuring that all work with pesticides at Cornell is conducted properly and legally rests on the individual user. (Section 12.0)
- When using pesticides in a non-dispersive manner in a laboratory setting, an individual must follow the safety rules outlined in the Cornell University Laboratory Safety Manual. (Section 12.1.1)
- It is the responsibility of the Principal Investigator or laboratory supervisor to ensure biological safety cabinets within laboratories under their supervision are certified annually. (Section 13.5.1)
- It is the responsibility of the Principal Investigator or laboratory supervisor with class 3b or 4 LASERs in laboratories under their supervision to ensure the class 3b or 4 LASERs have been registered with EHS and employees using these LASERs have received the appropriate training. (Section 15.0)
- It is the responsibility of the Principal Investigator and laboratory supervisor to ensure that staff and students in laboratories under their supervision are provided with adequate training and information specific to the physical hazards found within their laboratories. (Section 16.0)
Appendix D - Standard Operating Procedure ExamplesAppendix D - Standard Operating Procedure Examples
The following are Cornell SOP examples:
- Acetic anhydride
- Aliphatic hydrocarbons
- Dimethyl sulfoxide (DMSO)
- Ether, Diethyl ether
- Halogenated solvents
- Heavy metal salts
- Low molecular weight organic acids
- Phenol Template (docx)
The following links are examples of SOPs from other university websites:
- A list of SOP examples and resources on the web from the University of Maryland
- The SOP library (with numerous examples) from the University of California - Irvine
- The Michigan State University SOP webpage (with a number of examples)
- An example of a chemical list SOP (generic – not lab specific) – University of Pennsylvania
Appendix E - Lab Decommissioning ProcessAppendix E - Lab Decommissioning Process
The primary responsibility for the proper disposal or transfer of hazardous materials used in laboratories lies with the Principal Investigator or Researcher so that all substances and equipment are safely moved, or disposed of, and the space has been appropriately decontaminated. Researchers are encouraged to consult the University Lab Safety Manual and Chemical Hygiene Plan, Biosafety Manual, Radiation Safety Manual, and the Hazardous Waste Manual, for guidance on the proper disposition of research materials. Also, departments are responsible for ensuring that research spaces are vacated in an organized fashion, which enables the space to turn over to construction crews or the next occupant free from hazards. This guide provides standardized processes, strategies, and validation methods for screening and characterization of hazardous debris, proper decontamination of workspaces, and compliance with hazardous waste regulations. This process is used whether the lab move is within the university or to another off-campus location.
Researcher/ Principal Investigator
Notify the department chair, department safety representative, the facility/building coordinator and Environment, Health, and Safety (EHS) through askEHS@cornell.edu of the intent to move or close the lab. Discuss needs, concerns, and timelines of lab decommissioning with your facility coordinator, Department Safety Representative (DSR), and EHS. Several months of notice and work are generally required to properly decommission a laboratory for move out, renovation or re-occupancy.
Provide area managers and EHS information on historical use of biohazardous materials, radioactive materials, and hazardous chemical usage for decontamination analysis.
Plan early for the disposal of hazardous materials such as chemicals, microorganisms, tissues, and radioactive materials. Provide chemical inventory to EHS to aid in the relocation or disposal of unwanted chemicals.
Ensure that materials generated in the conduct of research by individuals working in the lab have been properly identified or disposed of prior to them leaving the University.
Ensure that research material cleanouts are performed by staff knowledgeable of hazards and appropriately trained in required safety procedures.
Adhere to established EHS procedures for the safe and compliant disposal and decontamination of research materials and equipment.
Maintain assigned facilities in clean, safe, and operable conditions during the lab closeout process. Corridors and other egress paths must remain free of trash or hazardous materials. Access to emergency equipment inside or near the lab space (Eyewash, Safety Shower, Fire extinguisher) must remain unimpeded.
Ensure that contamination resulting from research activities or clean out has been appropriately cleaned and removed prior to vacating assigned spaces. All benchtops, cabinets, drawers, and shelving must be cleaned with detergent and water and if biological agents have been used, apply an appropriate disinfectant.
Complete Laboratory Decommissioning Checklist and Research Equipment Decommissioning Form and provide them to EHS, the DSR, Facility/Building Coordinator, or other college representatives.
Negotiate the disposition of laboratory equipment with the Department or Research Center Administrator.
Acknowledge notice from PI, or originator, of intent to move or close the lab. Provide all appropriate checklists and forms. Set up a Teams site for communication and set-up a task tracking plan to track the project activities based on the checklist. Provide a point of contact for support of the decommission activities.
Provide guidance on applicable federal, state, and local regulatory requirements for decommissioning of laboratories.
Provide technical guidance and advice on decontamination and hazardous material disposal.
Assist with risk assessments for regulatory compliance and safe work practices.
Provide materials such as boxes and hazardous waste tags to assist in the preparation of chemical hazardous wastes for removal.
Upon request, schedule removal of appropriately labeled and packaged wastes destined for disposal.
Provide continual review of project decommissioning as new information is obtained.
Provide a final walkthrough of the lab to verify space is clean and decontaminated and that equipment for disposal or moving has been adequately decontaminated and hazard signs have been removed.
Deans, Department Chairs, and/or Unit Directors
Allocate space appropriately and match research safety needs with facility capabilities.
Communicate assigned spaces or changes in assignments to Researchers/Principal Investigators and facilities management staff.
Inform the Principal Investigator of responsibilities related to management of research spaces and materials.
Notify Facility Director or Manager/ Building coordinator of pending changes to lab occupancy.
Assure that vacated spaces have been cleared by EHS, the Safety Manager and/or Facility Manager prior to renovation or reassignment.
Bear the costs of decontamination and disposal of research materials in instances where there has been a failure by the PI to meet their responsibilities or when costs cannot be recovered from the investigator.
Research Center Administrator/Department Administrative Manager
Ensure department interests in are represented in the space decommissioning process.
Ensure there is communication of a timeline for space decommissioning given faculty status.
Ensure that department assets are appropriately inventoried and reallocated.
Ensure that spaces are returned to the department in a timely manner and in satisfactory condition.
Ensure the HASP door sign is updated to reflect the current hazards (i.e. if empty, none) and the responsible person.
Submit change of occupancy to FCS space inventory
Facilities Manager / Building Coordinator
Acknowledge notice from PI of intent to move or close the lab. Ensure that EHS, Safety Manager, Facility Director, Department Chair is informed of any potential changes in lab occupancy
Be knowledgeable in the decommissioning process.
Assist EHS, Safety Manager, and/or Facility Director to coordinate the decommissioning process
Assist the PI and research group in managing repair requests, locating equipment, and clarifying expectations as they relate to University policies and practices.
Evaluate and provide guidance on packaging, moving, or relocating hazardous materials used in research.
Arrange for proper decommissioning and decontamination of abandoned laboratory spaces.
Review and discuss costs of decommissioning activities, including labor charges to properly segregate and label hazardous material with the Department Chair or Administrator. Discuss options for these activities with EHS.
Perform a visual inspection of the space to verify that the space is free of hazardous materials and contamination prior to being released to the department.Ensure the space has been cleared by EHS, the Safety Manager and or Facility Director prior to reassignment or renovation
Laboratory Decommissioning Guidance:
The steps listed below must be taken whenever a PI or other Lab Supervisor plans to vacate their laboratory space. Laboratory space cannot be re‐occupied, nor renovation work started, until the space has been inspected and cleared by all the relevant groups from Environment Health and Safety (EHS) (Lab/Chemical Safety, Biosafety, Radiation Safety, Hazardous Waste as appropriate).
- Upon notification from, or to, the Department Chair or Administrator of a change in lab occupancy, the department or PI should contact EHS to set up a meeting to be held with the PI or researcher, Department Representatives, and Facility Manager to establish a schedule and identify appropriate action items.
- The Safety Manager or Facilities Manager and Unit Capital Assets Manager will review the relevant spaces to determine the presence and disposition of capital assets and develop a list of actionable items for the Department, PI, and staff.
- The findings of the initial review will be provided to the faculty member and the Department Administrator.
- The PI and his or her team will work to address the items identified in the initial review along with items described below and complete the Laboratory Close‐out Checklist.
- If a PI is unable to address the action items identified in the initial site review, the department will implement an assisted cleanout process which entails obtaining bids for the costs of the cleanout and providing this information to the Department Administrator for approval.
- EHS staff from the relevant chemical, biological and radiation safety groups will conduct a final walkthrough of the spaces. If spaces are found to be in unsatisfactory condition, written notification to this effect will be provided to the PI and Department detailing the remaining action items. If the spaces are found to be in satisfactory condition, written notification will be provided to the PI and the department who will then update and post the HASP signage to reflect lab vacancy.
- Create a plan to remove all items from the lab (or scheduled to be removed), including items in drawers, cabinets, fume hoods, refrigerators, freezers, etc. Dispose of items safely. Be considerate of custodial staff and maintenance staff who may not understand the hazards of the lab work your group conducted.
- Surplus tables, cabinets, etc. that are no longer needed or wanted, should be labelled and the information on these items should be provided to your Facility Manager/Coordinator to see if these items could be donated to someone else in your department. Once the items have been entirely cleaned and decontaminated, the Facility Manager/Coordinator should arrange for transfer of these items to new areas or tagged for disposal
- Clean out refrigerators and freezers and defrost freezers. Samples must be disposed of appropriately according to the hazards they contain.
- Ensure all surfaces have been thoroughly cleaned with detergent and water. Bench tops, storage cabinets, and drawers (both inside and outside), shelving, and the outside of large equipment that is scheduled to be moved. Areas potentially contaminated with biohazards such as bench tops, refrigerators, centrifuges, must be cleaned with a disinfectant appropriate to the equipment and organisms used in the lab.
- Identify contaminated area(s) that cannot be cleaned by researchers and work with DSR and EHS to facilitate decontamination of the area(s). This includes biosafety cabinets, fume hoods and other equipment.
- The following items are picked up by the R5 group (607-255-1666) – used oil, batteries, fluorescent bulbs, lead sheeting and block.
- Return all keys to your Key Control Coordinator and provide them with your contact information in case questions arise.
- Update your current chemical inventory to aid in detailing decisions as you go through the process of decommissioning your laboratory. Questions about what hazardous waste is and what can be donated to groups within the department can be more easily answered.
- All chemicals that are not being taken to a new lab space should be separated into those going to be repurposed to another lab or disposed of through hazardous waste.
- EHS can provide research groups with information and assistance with segregation and proper packaging of hazardous chemicals.
- EHS cannot accept waste containers that are broken, have broken tops, or that have glass stoppers or other non-sealable lids. Material can be transferred to a compatible container or over packed within another sealable container.
- For questions about proper disposal of hazardous waste contact EHS at “askEHS”.
- Chemicals should be moved only by trained individuals. Any highly toxic, highly hazardous, or reactive chemicals should only be moved by staff who have received special training. When moving highly toxic or highly hazardous chemicals, EHS recommends a "buddy system" be used in the event of a spill or other emergency.
- When moving chemicals, be sure all containers are properly labeled, and all are securely closed. When transporting chemicals, use DOT approved shipping containers and secondary containment.
- There are special regulations associated with transporting hazardous chemicals off campus, see the EHS webpage for Hazardous Materials Shipping for more information. Hazardous materials must not be transported in a personal vehicle. When packaging chemicals, use a packing material (such as vermiculite, ground corn cobs, shipping peanuts, cardboard, absorbent clay, etc.) that is compatible with the chemicals to prevent bottle breakage during transport. Only place chemicals that are compatible with each other in the same container and do not overload containers of chemical bottles.
- When transporting chemicals, use carts with lips or trays to prevent containers from being knocked off. Other items that are useful for transport include rubber bottle carriers, five-gallon pails, or other forms of secondary containment.
- When moving chemicals, wear appropriate personal protective equipment such as safety glasses or splash goggles, lab coat, and gloves. Remove gloves when touching doorknobs and latches, and elevator buttons. If possible, avoid using passenger elevators. If you must use a passenger elevator, request that no passengers ride along with you.
- Reactive earth metals (sodium and lithium, etc.) need to be packaged in a volume of 1 pound or less (not counting the jar and oil). One pound per container is the maximum that the waste vendor can and will take for safety reasons.
- Fume hoods must be cleaned of debris and a surface decontamination completed. If there is significant contamination within the ductwork, this must be communicated with facility/department managers and EHS so that an outside vendor may be hired to clean the ductwork of specific contaminants.
- If laboratory work involved Select Agents or Toxins, consult with EHS Biosafety prior to conducting any work in preparation for the move. Specific procedures must be followed.
- Communicate in writing, to the Cornell Office of Research and Sponsored Programs, Institutional Biosafety Committee, Institutional Animal Care and Use Committee, or Institutional Review Board to discontinue or modify all approved protocols, as appropriate.
- If a USDA or CDC permit is associated with your laboratory operations, all related materials must be destroyed, or the permit updated with the regulatory authorities to allow for intra- or inter-facility transport.
- If the lab works with plants, contact the appropriate facility managers to transfer plants to other PIs or arrange for destruction of materials.
- If the laboratory has been involved in work with live animals, contact the Center for Animal Resources and Education.
- Biohazardous materials must be properly packaged and only moved by properly trained laboratory staff. Non-laboratory personnel (including moving company staff) or untrained laboratory personnel are not permitted to move biohazardous materials. Transfer permitting requirements may also apply (e.g., CDC or USDA permits) depending on the agent.
- All surfaces and equipment from laboratories must be decontaminated with an appropriate disinfectant. Appropriate choice of disinfectant may be determined by
- Referring to the approved IBC Memorandum of Understanding and Agreement,
- Contacting the Biosafety Office at askEHS@cornell.edu, or
- Consulting Pathogen Safety Data Sheets
- Referring to the EPA’s website for selected registered disinfectants. Appropriate use may be determined by referring to the manufacturer’s requirements for the use and contact time.
- If you have a Biosafety Cabinet (BSC) thoroughly decontaminate both the inside and outside of the cabinet. Biosafety cabinets that are moved to another location must be gas or vapor decontaminated by an appropriate vendor in addition to surface decontamination performed by researchers or lab staff. Cornell University has a contract with Steris Life Sciences, please schedule directly with them at email@example.com.
Animal and Human Tissues:
This includes animal carcasses, tissues or animal or human origin.
- Separate tissue from liquid preservative. Liquid preservatives may be hazardous waste. Do not assume that preservatives may be flushed down drains. Contact the Safety Manager if you have questions.
- Place any tissues of human origin, animal tissues, cell lines, Risk Group 2 organisms or other infectious tissue in a red biohazard waste bag per University Regulated Medical Waste procedures.
- Regulated medical waste includes cultures and stocks of infectious agents, culture dishes and devices used to transfer, inoculate or mix cultures that have come into contact with or are known to be contaminated with biological agents infectious to humans, or agents of economic concern (e.g., foreign animal diseases); human pathological wastes, body fluids, human blood and blood products or components, animal waste, including animal carcasses, body parts, body fluids, blood or bedding originating from animals known to be contaminated with infectious agents.
- Decontaminate all waste materials and unwanted stocks, generally by autoclaving. This may involve disposal of regulated medical waste or recombinant materials through the appropriate waste pipeline. Devitalize arthropods by freezing before autoclaving.
- Recombinant materials are to be disposed as appropriate. Please refer to the Laboratory Waste Disposal Guide for more details on appropriate waste pathways.
- Submit a request to EHS for the disposal of regulated medical waste. To request a pickup, fill out the form on the EHS website.
- Contact the EHS Biosafety Officer for a final walkthrough. The biosafety office will remove agent placards/signage from doors, equipment, etc. at the time of the final walkthrough and upon demonstration that decommissioning has been completed.
Refer to Radiation Safety Manual Section 9.4 for Disposal of Radioactive Material and the Waste Procedure for Disposal of Radioactive Materials.
NOTE: All the following steps must be coordinated through one of the EHS staff members from the Radiation Safety Group. Advance notification of your planned move is required, contact askEHS@cornell.edu.
If any equipment contains any radioactive material (e.g., static eliminators in balances, electron capture devices in gas chromatographs), X-rays, or lasers, contact radiation safety in EHS for assistance with transfer or disposal. These items may be on university inventory lists that must be maintained accurately. Identify any new locations for these items.
Contact EHS to remove any stocks, samples, and contaminated supplies.
Properly dispose of any radioactive waste. To request a radioactive material waste pickup, fill out the form on the EHS website: Waste Pickup.
Perform a complete survey using a calibrated meter, including all authorized rooms, equipment, and high touch areas.
- All vacated rooms must be certified as contamination free before they are turned over to custodians, maintenance workers, or new lab occupants.
For the removal of equipment that has been used with radioactive materials, these must be certified as contamination-free before the equipment is handled by movers and not by laboratory staff.
Only properly trained staff may move radioactive materials and small equipment used with radioactive materials. All materials must be properly packaged and shielded.
If there is significant contamination within the fume hood ductwork, this must be communicated with area managers and EHS so that an outside vendor may be hired to clean.
- Before moving any compressed gas cylinders, remove the regulator and replace the safety cap over the cylinder valve. Only use an appropriate cylinder handcart to move compressed gas cylinders. Do not attempt to "roll" or “walk” cylinders from one area to another.
- Any compressed cylinders containing highly toxic or highly reactive gases should only be moved by staff with special training in the use and hazards of these materials.
- Do not leave compressed gas cylinders unsecured at any time.
- If possible, avoid using passenger elevators. If you must use a passenger elevator do not ride in the elevator with the gas cylinder.
Occasionally it is necessary to dispose of materials that may contain more than one hazard. Contact EHS at 607-255-8200 for information on the disposal of any combination of bio-hazardous materials, and chemicals and/or radioactive materials.
Export of Materials or Data out of the Country:
Contact the Cornell University Export Controls Office
Computers & Electronics:
Refer to list of materials and recycling guidelines for R5.
- Work with the department equipment asset coordinator to determine the fate of equipment remaining in the spaces.
- Decontaminate computers or electronics that are potentially contaminated with hazardous materials prior to disposal.
- Dispose of or remove personal computers and other non-fixed electronics not slated for disposal.
- Notify the Facility Manager/ Coordinator if you suspect that an item may contain hazardous materials such refrigerants, coolants or, PCB oil, etc.
- Contact others to determine if items can be used by another researcher.
Laboratory Decommissioning Checklist
- Unwanted materials in beakers, flasks, evaporating dishes, etc. have been disposed via appropriate means.
- A hazardous waste pickup request has been placed by filling out the form on the EHS website.
- Small bottle(s) have been placed in a Ziplock bag and the waste label attached to the Ziplock bag.
- Unwanted chemicals that could potentially be used by other labs are segregated and identified. Department safety representative has been contacted to facilitate any transfer of chemicals.
- Chemicals that will be repurposed to another lab are in clean original containers.
- Chemicals transferred to another owner have been incorporated into the new owner’s chemical inventory.
- Materials or chemicals to be retained are properly labelled and stored in appropriate containers.
- Chemicals are segregated in accordance with compatibility, packed into a sturdy container/box and labelled for transportation.
- Lab has been searched including drawers, cabinets, refrigerators, and freezers to make sure all samples and chemicals have been identified.
- Completed a new chemical inventory for the items to be retained.
- Materials or chemicals to be retained are properly labelled and stored in appropriate containers.
Compressed Gas Cylinders
- Gas connections have been removed, cylinder caps replaced, and cylinders placed in an appropriate area for pick up.
- EHS has been contacted for disposal of any lecture bottles or non-returnable cylinders such as those obtained through military surplus
- Gas vendor contacted and pickup arranged for the removal of any compressed gas cylinders that will no longer be used or for return of any empty cylinders. If you need assistance having the cylinders removed, contact your Department Safety Representative or Facility Manager/Coordinator.
The US Drug Enforcement Agency (DEA) issues controlled substance permits to individual researchers. Abandonment of a controlled substance is a violation of the DEA permit under which it was held. Permission to dispose or transfer ownership of a controlled substance to another individual must be received from DEA.
- Investigators leaving Cornell University must properly dispose of controlled substances through reverse distribution prior to expiration/surrender of state license and DEA registration and prior to leaving Cornell University. EHS has been contacted through askEHS@cornell.edu to arrange for reverse distribution.
- Documentation of disposal must be kept and maintained in records log for 2 years with the department and EHS. Documentation has been forwarded to department and EHS.
- All Senior authorized users and authorized users have had their access revoked and signed off by licensee/registrant in records.
- Licensee/Registrant has contacted proper agencies to surrender Active NYSDOH license and DEA registration.
- Keys to lockbox and/or combinations to safe or key boxes have been given to department business office and/or EHS once controlled substances have been removed.
- Records / Logbook have been handed in to department and maintained for a minimum of 2 years following licensee/registrant’s surrender of license/registration.
- EHS has been contacted for completion of a final inspection for controlled substances prior to licensee/registrant’s final working day.
- Unwanted samples and specimens have been rendered non-viable by a method appropriate for the organisms present.
- All material from centrifuges, incubators, refrigerators/freezers/ liquid nitrogen dewars has been removed.
- Regulated medical waste pickup request has been submitted to EHS and has been scheduled or pick up has already occurred.
- Liquid media, cultures, human bodily fluids, or other liquid potential infectious materials have been autoclaved or treated with an appropriate disinfectant for the recommended contact time and then drain disposed.
- Biohazardous materials are properly packaged and only moved by properly trained laboratory staff. Non-laboratory personnel (including moving company staff) or untrained laboratory personnel are not permitted to move biohazardous materials.
- Infectious or recombinant materials to be retained at Cornell have been transferred to an active IBC Protocol.
- Biosafety Cabinet (BSC) is thoroughly decontaminated both inside and outside with an EHS or IBC approved disinfectant. An external vendor has disinfected the filter of the unit with vaporized hydrogen peroxide or formaldehyde. Users will not attempt to do this.
- After moving to a new location, BSC has been recertified by a third-party vendor. Check with the manufacturers guidelines before moving your BSC.
- Non-flammable gas lines have been disconnected.
- Biosafety Cabinet (BSC) is thoroughly decontaminated both inside and outside with an EHS or IBC approved disinfectant. An external vendor has disinfected the filter of the unit with vaporized hydrogen peroxide or formaldehyde. Users will not attempt to do this.
- Benchtops and all equipment (e.g., centrifuges, incubators, refrigerators/freezers/ liquid nitrogen dewars) have been cleaned and disinfected, with an EHS approved disinfectant.
- Guidance for the decontamination of other equipment has been reviewed and signage attached indicating it is decontaminated.
- Biohazardous sharps have been placed in red sharps container which must be labeled with group contact information and placed in RMW accumulation. Biohazardous sharps include all hypodermic needles and syringes, broken or unbroken plastic ware, glass, or sharp metal that has been in contact with infectious material or products of human or mammalian origin.
- Scheduled a waste pickup of sharps containers.
Residual amounts of Chemotherapeutic Agents
- This includes antineoplastic agents, hormones or hormone-like drugs, synthetic analogs and other carcinogens used in molecular biological applications (e.g., BrdU).
- Placed in yellow chemotherapeutic waste container (rigid container, bag) and deposited in the Regulated Medical Waste bin.
- Radiation Safety has been contacted to assist with termination of the Radioactive Use Permit - askEHS@cornell.edu
- Scheduled a waste pickup to dispose all remaining stocks, samples, waste, and contaminated supplies.
- Used a calibrated meter to perform a complete survey of the entire area and documented results. Unshielded dose rates must be ALARA below the regulatory limits specified on the survey form.
- Documented all results, both before and after any decontamination.
- Disposed of materials used for decontamination as radioactive waste.
- Filed the final contamination survey results with the laboratory’s monthly survey records and send a copy to EHS.
- Scheduled a visit with EHS radiation safety staff to conduct the terminal survey for fixed and removeable contamination.
- EHS removed all material, performed, and documented an in-lab “final” contamination survey, including all authorized rooms and labeled equipment as well as high-touch locations like doorknobs, faucet handles, etc.
- EHS removed any final radioactive waste.
- EHS removed all remaining radioactive material postings.
- All Permit documentation has been given to EHS for archiving and compliance with NYS record retention requirements.
- Fume hoods are clean of debris and a surface decontamination with detergent has been completed. If there is significant contamination within the ductwork, this has been discussed with Facility Manager/Coordinator and EHS so that an outside vendor may be hired to clean the ductwork of specific contaminants.
- EHS, Facilities Manager/Coordinator and Cornell Facilities Management have been alerted to any exhaust or filtration equipment used with extremely hazardous substances or organisms.
- EHS has removed, defaced, or covered hazard labels on equipment to be moved or discarded.
- All stored electrical, mechanical, pressure hazards have been discharged.
- Department representative has been contacted to remove any unwanted equipment after it has been decontaminated.
- Cleaned all work and storage surfaces with detergent and water, with special attention given to areas with visible contamination.
- Broken glass boxes disposed.
- Floors are swept and are “broom clean” (e.g., no spills of hazardous materials present).
- Notified Facility Manager/ Coordinator when laboratory clean‐up is complete.
- Inspected lab including drawers, cabinets, refrigerators, and freezers to make sure all hazards have been accounted for.
- Returned keys to Key Control Coordinator and provided them with your contact information in case questions arise.
- Submitted this form to EHS, College Safety Representative and Department Representative.
I have completed the decommissioning of my laboratory, properly disposed of all hazardous material, and appropriately decontaminated all surfaces and equipment.
PI signature ________________________________________ Date________________
Appendix F - Glove Selection GuidanceAppendix F - Glove Selection Guidance
Ansell Protective Products – SpecWare Online Chemical Hand Protection
This provides a step by step search for the selection of proper gloves based on the chemicals intended to be used. Ansell conducts standardized testing of glove materials and regularly updates its recommendations.
This link provides helpful hints and definitions that are important when selecting gloves to use when working with chemicals.
This link also provides helpful hints and answers to common questions.
This link provides a quick reference chart and simple hazard assessment steps to follow. This information is regularly updated.
Appendix G - EHS Video LibraryAppendix G - EHS Video Library
EHS has developed a safety video YouTube channel. We expect to continue to add to this library, so if you have suggestions about topics for videos that would provide useful to your constituencies, please let us know at askEHS@cornell.edu and we'll do our best to meet this need.
In addition, we maintain 3 Twitter feeds:
@cornelllabsafe - laboratory safety, nanotech and green chemistry news
@acsdchas - hazmat incidents and information
@labsustain - news related laboratory sustainability
All three of these twitter feeds are active, with 3 to 5 tweets per day.
We also recommend these videos from other campuses:
- A Day in the Lab (University of California San Diego): This video describes the importance of laboratory safety from the perspective a Principal Investigator.
- Working with Pyrophoric Reagents (from University of California, San Diego): This page has three videos:
- Part 1: Getting Ready
- Part 2: Transferring Pyrophoric Liquids
- Part 3: Working with Reactive Metals
- Flash Chromatography (from University of California, San Diego Environmental Health and Safety): The following video provides a brief overview of the hazards associated with flash chromatography.
- Experimenting with Danger (24:05) US Chemical Safety Board overview of academic lab safety. This video describes hazards associated with research at chemical laboratories in academic institutions.
- Carbon Nanotubes Risks - exposure, chemistry and physical form (University of Michigan Risk Science Center) An overview of the factors to consider in assessing health risks associated with carbon nanotubes
Additional Laboratory Safety Resources:
- The video version of GHS Updates for Cornell Labs
- Cornell University Biological Safety Manual
- Cornell University Laboratory Safety Manual
- Biosafety in the Laboratory
- American Chemical Society, Safety in Academic Chemistry Laboratories
- Prudent Practices in the Laboratory from the National Research Council
- Identifying and Evaluating Hazards in Research Laboratories
Appendix H - How To Understand SDSsAppendix H - How To Understand SDSs
Chemical manufacturers are required by law to supply "Safety Data Sheets" (OSHA Form 174 or its equivalent) upon request by their customers. These sheets have nine sections giving a variety of information about the chemical. Following is a section-by-section reproduction and explanation of a Safety Data Sheet (SDS).
|U.S. DEPARTMENT OF LABOR|
|Occupational Safety and Health Administration|
|SAFETY DATA SHEET|
|Required For compliance with OSHA Act of 1970|
|Public Law 91-596 (CFR 1910)|
|For Information on Health Hazards Call|
|For Other Information Call|
|Signature and date|
This section gives the name and address of the manufacturer and an emergency phone number where questions about toxicity and chemical hazards can be directed. Large chemical manufacturers have 24-hour hotlines manned by chemical safety professionals who can answer questions regarding spills, leaks, chemical exposure, fire hazard, etc. Other information that may be contained in Section I includes:
Trade Name: This is the manufacturer's name for the product.
Chemical Name and Synonyms: This refers to the generic or standard names for the chemical.
Chemical Family: This classification allows one to group the substance along with a class of similar substances, such as mineral dusts, acids, caustics, etc. The potential hazards of a substance can sometimes be gauged by experience with other chemicals of that hazard class.
SECTION II - HAZARDOUS INGREDIENTS OF MIXTURES
|Principal Hazardous component(s)||%||TVL (Units)|
This section describes the percent composition of the substance, listing chemicals present in the mixture. It lists Threshold Limit Values for the different chemicals that are present. Threshold Limit values (TLV's) are values for airborne toxic materials that are used as guides in the control of health hazards. They represent concentrations to which nearly all workers (workers without special sensitivities) can be exposed to for long periods of time without harmful effect. TLV's are usually expressed as parts per million (ppm), the parts of gas or vapor in each million parts of air. TLV's are also expressed as mg/m3, the milligrams of dust or vapor per cubic meter of air.
SECTION III - PHYSICAL DATA
|Boiling Point (oF)||Specific Gravity (H2O=1)|
|Vapor Pressure (mm Hg)||Percent Volatile By Volume (%)|
|Vapor Density (Air=1)||Evaporation Rate (Butyl Acetate=1)|
|Solubility in Water|
|Appearance and Odor|
This section gives information about the physical characteristics of the chemical. This information can be very useful in determining how a chemical will behave in a spill situation and what appropriate steps should be taken.
Vapor Pressure: Vapor pressure (VP) can be used as a measure of how volatile a substance is…how quickly it evaporates. VP is measured in units of millimeters of mercury (mm Hg). For comparison, the VP of water (at 20o Centigrade) is 17.5 mm Hg. The VP of Vaseline (a nonvolatile substance) would be close to zero mm Hg, while the VP of diethyl ether (a very volatile substance) is 440 mm Hg.
Vapor Density: Vapor density describes whether the vapor is lighter or heavier than air. The density of air is 1.0. A density greater than 1.0 indicates a heavier vapor, a density less than 1.0 indicates a lighter vapor. Vapors heavier than air (gasoline vapor for instance) can flow along just above the ground and can collect in depressions where they may pose a fire and explosion hazard.
Specific Gravity: Specific gravity describes whether the liquid is lighter or heavier than water. Water has a specific gravity of 1.0.
Percent Volatile by Volume: Describes how much of the substance will evaporate.
SECTION IV - FIRE AND EXPLOSION HAZARD DATA
|Flash Point (oF)||Flammable Limits in Air (% by Vol.)||Lower||Upper|
|Extinguisher Media||Autoignition Temperature (oF)|
|Special Fire Fighting Procedures|
This section gives information, which is important for preventing and extinguishing fires and explosions. If a fire does occur, this information should be made available to fire fighters.
Flash Point: Flash point is the lowest temperature at which a liquid gives off enough vapor to ignite when a source of ignition is present. A fire or explosion hazard may exist if the substance is at or above this temperature and used in the presence of spark or flame.
Flammable Limits: In order to be flammable, a substance must be mixed with a certain amount of air (as in an automobile carburetor). A mixture that is too "lean" (not enough chemical) or too "rich" (not enough air) will not ignite. The Lower Explosive Limit (LEL) and the Upper Explosive Limit (UEL) define the range of concentration in which combustion can occur. The wider the range between the LEL and UEL, the more flammable the substance is.
SECTION V - HEALTH HAZARD DATA
|Threshold Limit Value|
|Effects of Overexposure|
|Emergency and First Aid Procedures|
This section describes the potential health effects resulting from overexposure to the chemical and gives emergency and first aid procedures. The symptoms and effects listed are the effects of exposure at hazardous levels. Most chemicals are safe in normal use and the vast majority of workers never suffer toxic effects. However, any chemical can be toxic in high concentrations, and the precautions outlined in the MSDS should be followed.
The health hazards section often contains information on the toxicity of the substance. The data most often presented are the results of animal experiments. For example, "LD50 (mouse) = 250 mg/kg." The usual measure of toxicity is dose level expressed as weight of chemical per unit body weight of the animal-usually milligrams of chemical per kilogram of body weight (mg/kg). The LD50 describes the amount of chemical ingested or absorbed by the skin in test animals that causes death in 50% of test animals used during a toxicity test study. Another common term is LC50, which describes the amount of chemical inhaled by test animals that causes death in 50% of test animals used during a toxicity test study. The LD50 and LC50 values are then used to infer what dose is required to show a toxic effect on humans.
As a general rule of thumb, the lower the LD50 or LC50 number, the more toxic the chemical. Note there are other factors (concentration of the chemical, frequency of exposure, etc.) that contribute to the toxicity of a chemical, including other hazards the chemical may possess.
Health hazard information may also distinguish the effects of acute and chronic exposure. Acute toxicity is generally thought of as a single, short-term exposure where effects appear immediately and the effects are often reversible. Chronic toxicity is generally thought of as frequent exposures where effects may be delayed (even for years), and the effects are generally irreversible. Chronic toxicity can also result in acute exposures, with long term chronic effects.
SECTION VI - REACTIVITY DATA
|Stability||Unstable||Conditions to avoid|
|Incompatibility (Materials to Avoid)|
|Hazardous Decomposition Products|
|Hazardous Polymerization||Conditions to Avoid|
|May Occur||Will Not Occur|
This section gives information on the reactivity of the chemical – with other chemicals, air, or water which is important when responding to a spill or fire. Chemical substances may be not only hazardous by themselves, but may also be hazardous when they decompose (break down into other substances) or when they react with other chemicals.
Stability: Unstable indicates that a chemical can decompose spontaneously under normal temperatures, pressures, and mechanical shocks. Rapid decomposition may be hazardous because it produces heat and may cause a fire or explosion. Stable compounds do not decompose under normal conditions.
Incompatibility: Certain chemicals should never be mixed because the mixture creates hazardous conditions. Incompatible chemicals should not be stored together where an accident could cause them to mix.
Hazardous Decomposition Products: Other chemical substances may be created when a chemical burns or decomposes.
Hazardous Polymerization: Some chemicals can undergo a type of chemical reaction (rapid polymerization) which may produce enough heat to cause containers to explode. Conditions to avoid are listed in this section.
SECTION VII - SPILL OR LEAK PROCEDURES
|Steps to be Taken in Case Material is Released or Spilled|
|Waste Disposal Method|
This section can provide specific information about how to clean up a spill of the chemical and how the chemical should be properly disposed.
SECTION VIII - SPECIAL PROTECTION INFORMATION
|Respiratory Protection (Specify type)|
|Protective Gloves||Eye protection|
|Other Protective clothing or Equipment|
This section gives information for any special protection that needs to be taken when handling this chemical including ventilation requirements and the type of personal protective equipment that should be worn.
SECTION IX - SPECIAL PRECAUTIONS
|Precautions to be Taken in Handling and Storing|
This section describes other precautionary measures that may need to be taken. Some of the precautions presented are intended for large-scale users and may not be necessary for use with small quantities of the chemical. Any questions about precautions or health effects should be referred to EHS at 607-255-8200.
Appendix I - Hazards Of Functional GroupsAppendix I - Hazards Of Functional Groups
The following information gives a basic overview of the hazards of functional groups. This information is not meant to replace material safety data sheets for the specific chemical(s) used in your experiments. While these functional groups are listed alphabetically for convenience, chemicals should be segregated and stored by hazard classes – see the EHS Segregation Scheme for more information.
- The lower aliphatic alcohols are low to moderately toxic and usually have low vapor pressures, therefore inhalation toxicity is low.
- Vapors may be an irritant to the eyes and mucous membranes.
- Ingestion and absorption of the liquids through the skin can be a major health hazard.
- Lower alcohols containing double or triple bonds exhibit a greater degree of toxicity and irritation.
- Fatty alcohols (derived from oils, fats, and waxes) are almost nontoxic.
- Lower alcohols are flammable or combustible liquids.
- Flammability decreases with an increase in the carbon number.
- Solubility of alcohols decrease with increase in carbon chain length.
- Toxicity tends to decrease with an increase in carbon number.
- Allyl alcohol
- 2-Propyn 1-ol
Aldehydes are intermediate products in the conversion of primary alcohols to carboxylic acids or vice versa.
- The low molecular weight aldehydes are more toxic than the higher ones.
- Toxicity decreases with increase in the carbon chain length.
- Aromatic aldehydes are less toxic than low molecular weight aliphatic aldehydes.
- Low molecular weight aldehydes are highly flammable, with flammability decreasing with increasing carbon chain length.
- Low aromatic aldehydes are combustible or nonflammable liquids.
The toxicity of most aliphatic amines may fall in the low to moderate category.
- The health hazard from amines arises primarily from their caustic nature.
- All lower aliphatic amines are severe irritants to the skin, eyes, and mucous membranes.
- All of these compounds have a strong to mild odor of ammonia and their vapors produce irritation of the nose and throat.
- Aliphatic amines, especially the lower ones, are highly flammable liquids, many which have flashpoints below 0 degrees Celsius.
- The vapors are heavier than air.
- They react vigorously with concentrated mineral acids.
- The flammability decreases with an increase in the carbon number.
- The reactivity of amines in general, is low.
Aliphatic and Aclicyclic Hydrocarbons:
Organic compounds composed solely of carbon and hydrogen.
- Hydrocarbons may be classified into 3 broad categories:
- Open-chain aliphatic compounds
- Cyclic or alicyclic compounds of naphthalene type
- Aromatic ring compounds
- Open chain aliphatic hydrocarbons constitute alkanes, alkenes, alkynes, and their isomers. Alkenes or olefins are unsaturated compounds, characterized by one or more double bonds between the carbon atoms. Alkynes or acetylenic hydrocarbons contain a triple bond in the molecule and are highly unsaturated. An alicyclic hydrocarbon is a cyclic ring compound of 3 or more carbon atoms. Aromatics are ring compounds too, but are characterized by a 6 carbon atom unsaturated benzenoid rings.
- The toxicities of aliphatic and alicyclic hydrocarbons in humans and animals are very low.
- The gaseous compounds are all nontoxic and are simple asphyxiants.
- Lower hydrocarbons are highly flammable substances, an increase in the carbon number causes a decrease in flammability.
- It is the flammable properties that make hydrocarbons hazardous.
- The reactivity of alkanes and cycloalkanes is very low.
- Alkenes and alkynes containing double and triple bonds are reactive.
Alkali and Other Reactive Metals:
Alkali metals constitute Group IA of the periodic table.
- Alkaline-earth metals constitute Group IIA and are less active than the alkali metals.
- These can be water and/or air reactive.
- Several of these metals are flammable, too, but only in finely divided state.
- Reactions with water produce strong bases.
Water-soluble bases, mostly the hydroxides of alkali- and alkaline-earth metals.
- Certain carbonates and bicarbonates also exhibit basic properties but are weak bases.
- These compounds react with acids to form salts and water.
- The health hazard from concentrated solutions of alkalies arises from their severe corrosive actions on tissues.
- These compounds are bitter to taste, corrosive to skin and a severe irritant to the eyes.
- The toxicity of alkalies is governed by the metal ions.
- Hydroxides and carbonates of alkali-and alkaline-earth are noncombustible.
- Strong caustic alkalies react exothermically with many substances, including water and concentrated acids, generating heat that can ignite flammable materials.
- Lithium hydroxide
- Potassium hydroxide
- Potassium carbonate
- Sodium hydroxide
Compounds that contain one or more amino groups attached to an aromatic ring.
- These amines are similar in many respects to aliphatic amines.
- These amines are basic, but the basicity is lower to aliphatic amines.
- The health hazard from aromatic amines may arise in two ways:
- Moderate to sever poisoning, with symptoms ranging from headache, dizziness, and ataxia to anemia, cyanosis, and reticulocytosis.
- Carcinogenic, especially cancer of the bladder.
- Many amines are proven or suspected human carcinogens, among aromatic amines, ortho-isomers generally exhibit stronger carcinogenic properties than those of the para- and meta-isomers.
- Unlike aliphatic amines, the aromatic amines do not cause severe skin burn or corneal injury.
- The pure liquids (or solids) may produce mild to moderate irritation on the skin.
- Lower aromatic amines are combustible liquids and form explosive mixtures with air.
- Amines may react violently with strong oxidizing compounds.
Aromatics are a class of hydrocarbons having benzene-ring structures.
- Many polyaromatics are carcinogens.
- The acute toxicity of mononuclear aromatics is low.
- Inhalation of vapors at high concentrations in air may cause narcosis with symptoms of hallucination, excitement, euphoria, distorted perception, and headache.
- Benzene is the only mononuclear aromatic with possible human carcinogenicity and other severe chronic effects.
- With a greater degree of substitutions in the benzene ring and/or increase in the carbon chain length of the alkyl substituents, the flammability decreases.
Azides, Fulminates, Acetylides, and Related Compounds:
These compounds form highly explosive shock- and heat-sensitive salts with many metals.
- Structurally they differ from each other, but have similar detonating characteristics.
- While alkali metal azides are inert to shock, the salts for copper, silver, lead, and mercury are dangerously shock sensitive.
- Fulminates of heavy metals are powerful explosives.
- These compounds are highly sensitive to impact and heat.
- Acetylides of heavy metals are extremely shock sensitive when dry, whereas, the salts of alkali metals are fairly stable.
- Most azides, fulminates, acetylides, nitrides and related compounds are highly unstable and constitute an explosion hazard.
- Salts of Group IB and IIB metals are especially explosive.
- Azides of nonmetals, such as those of halogens or organic azides such as that of cyanogen, are also extremely shock sensitive.
- Some of these compounds may even explode on exposure to light.
- Cuprous acetylide
- Hydrazoic acid
- Lead azide
- Mercury fulminate
- Silver fulminate
- Silver nitride
- Sodium azide
Weak organic acids, their strength is much weaker than mineral acids.
- Toxicity of monocarboxylic acids is moderate to low and decreases with carbon chain length.
- Some of lower dicarboxylic acids are moderate to high toxicity, becoming less toxic with increasing carbon chain length.
- Low molecular weight carboxylic acids are combustible liquids.
- Aromatic acids are of low toxicity.
- Acetic acid
- Butyric acid
- Formic acid
- Methacrylic acid
- Oxalic acid
- Propionic acid
- Succinic acid
- Valeric acid
Epoxides, also called oxiranes and 1,2-epoxides.
- Exposure to epoxides can cause irritation of the skin, eyes, and respiratory tract.
- Low molecular weight epoxides are strong irritants and more toxic than higher ones.
- Inhalation can produce pulmonary edema and affect the lungs, central nervous system and liver.
- Many epoxy compounds have been found to cause cancer in animals.
- Lower epoxides are highly flammable.
- They also polymerize readily in the presence of strong acids and active catalysts, this reaction generates heat and pressure that may rupture closed containers.
- Therefore contact with anhydrous metal halides, strong bases, and readily oxidizable substances should be avoided.
- Butylene oxide
- Ethylene oxide
- Isopropyl glycidyl ether
Lower aliphatic esters have a pleasant fruity odor.
- The acute toxicity of esters is generally of low order, they are narcotic at high concentrations.
- Vapors are an irritant to the eyes and mucous membranes.
- Toxicity increases with an increase in the alkyl chain length.
- Lower aliphatic esters are flammable liquids, some have low flash points and may cause flashback to an open container.
- The vapors form explosive mixtures with air.
- The flash point increases with increase in the alkyl chain length.
- The reactivity of esters is low.
- Aromatic esters are similar in effects as aliphatic esters.
- Ethyl acetate
- Ethyl formate
- Methyl acrylate
- Methyl formate
- n-Propyl acetate
- (Aromatics) Methyl benzoate
- Methyl salicyate
Widely used as solvents.
- They have a high degree of flammability.
- They tend to form unstable peroxides, which can explode spontaneously or upon heating.
- The flash point decreases with increase in carbon chain.
- Lower aliphatic ethers are some of the most flammable organic compounds and can be ignited by static electricity or lightning.
- The vapor densities are heavier than air.
- They form explosive mixtures with air.
- Aromatic ethers are noncombustible liquids or solids and do not exhibit the flammable characteristics common to aliphatic ethers.
- Ethers react with oxygen to form unstable peroxides, this reaction is catalyzed by sunlight, when evaporated to dryness, the concentrations of such peroxides increase, resulting in violent explosions.
- The toxicity of ethers is low to very low, at high concentrations these compounds exhibit anesthetic effects.
- Butyl vinyl ether
- Ethyl ether
- Isopropyl ether
- Methyl propyl ether
- Vinyl ether
Also known by the name Cellosolve.
- The toxic effects are mild, however, moderate to severe poisoning can occur from excessive dosage.
- The routes of exposure are inhalation, ingestion, and absorption through the skin.
- Compounds with high molecular weights and low vapor pressures do not manifest an inhalation hazard.
- Low molecular weight alkyl ethers are flammable or combustible liquids forming explosive mixtures with air.
- The reactivity of glycol ethers is low.
- There is no report of any violent explosive reactions.
- The high molecular weight compounds are noncombustible.
- Ethylene glycol monobutyl ether
- Ethylene glycol monomethyl ether
Haloethers are ethers containing hydrogen atoms.
- Halogen substitutions make ether molecules less flammable or nonflammable.
- The explosion hazards of low aliphatic ethers due to peroxide formation are not manifested by the haloethers. The halogens inhibit the ether oxidation to peroxides.
- Inhalation of Fluoroethers can produce anesthesia similar to that of the lower aliphatic ethers. Lower aliphatic chloro-and bromoethers can be injurious to the lungs.
- Many of these are cancer causing to lungs in animals or humans.
- Aromatic chloroethers are toxic by inhalation, ingestion, and skin absorption only at high doses. These effects can be attributed to the chlorine content and to a lesser extent on the aromaticity of the molecule.
- 2-Chloroethyl vinyl ether
The flammability of these compounds shows a wide variation.
- Bromo compounds are less flammable than their Chloro- counterparts, the difference in flammability is not great though.
- An increase in the halosubstitutions in the molecule increases the flash point.
- The flammable hydrocarbons are stable compounds with low reactivity.
- These compounds, however, may react violently with alkali metals and their alloys or with finely divided metals.
- Violent reactions may occur with powerful oxidizers, especially upon heating.
- Volatile halocarbons may rupture glass containers due to simple pressure build up or to exothermic polymerization in a closed vessel.
- Halogenated hydrocarbons in general exhibit low acute toxicity.
- Inhalation toxicity is greater for gaseous or volatile liquid compounds.
- The health hazard from exposure to these compounds may be due to their anesthetic actions; damaging effects on liver and kidney; and in case of certain compounds, carcinogenicity.
- The toxic symptoms are drowsiness, lack of coordination, anesthesia, hepatitis, and necrosis of the liver.
- Vapors may cause irritation of the eyes and respiratory tract.
- Death may result from cardiac arrest due to prolonged exposure to high concentrations.
- Ingestion can produce nausea, vomiting, and liver injury.
- Fluorocarbons are less toxic than the chloro-, bromo-, and iodo- compounds, the toxicity increases with increase in the mass number of the halogen atoms.
- Some of the halogenated hydrocarbons cause cancer in humans.
- Benzyl chloride
- Carbon tetrachloride
- Ethyl bromide
- Methylene chloride
The single most hazardous property of hydrides is their high reactivity toward water.
- The reaction with water is violent and can be explosive with liberation of hydrogen.
- Many hydrides are flammable solids that may ignite spontaneously on exposure to moist air.
- Many ionic hydrides are strongly basic; their reactions with acids are violent and exothermic, which can cause ignition.
- Hydrides are also powerful reducing agents, they react violently with strong oxidizing substances, causing explosions.
- Covalent volatile hydrides such as arsine, silane, or germane are highly toxic.
- Ionic alkali metal hydrides are corrosive to skin, as they form caustic alkalies readily with moisture.
- Lithium aluminum hydride
- Potassium hydride
- Sodium borohydride
- Sodium hydride
The toxic effects of most of the solvents are of low order, chronic exposures or large doses can produce moderate to severe poisoning.
- Most organic solvents are flammable or combustible liquids, the vapors of which can form explosive mixtures with air.
- Many of the common solvents can cause flashback of the vapors, and some form peroxide on prolonged storage, especially those compounds containing an ether functional group, some also can form shock-sensitive solvated complexes with metal perchlorates.
- Carbon tetrachloride
- Methyl acetate
Inorganic cyanides are the metal salts of Hydrocyanic acid.
- Cyanides of alkali metals are extremely toxic.
- In addition to being extremely toxic by ingestion or skin absorption, most metal cyanides present a serious hazard of forming extremely toxic Hydrogen cyanide when they come into contact with acids.
- Barium cyanide
- Cyanogen chloride
- Cyanamide cyanogen
- Hydrogen cyanate
- Sodium cyanide
- Potassium cyanide
Similar to aldehydes.
- In general, the toxicity is much lower than that of other functional groups, such as cyanides or amines.
- Unlike aldehydes and alcohols, some of the simplest ketones are less toxic than the higher ones.
- Beyond 7 carbons, the higher ones are almost nontoxic.
- Substitution of other functional groups can alter toxicity significantly.
- The simplest ketones are highly flammable.
- The flammability decreases with increase in the carbon number.
- Mesityl oxide
- Methyl Ethyl Ketone
Acid strengths vary widely.
- Sour in taste.
- React with a base to form salt and water.
- Produce hydrogen when reacting with most common metals.
- Produce carbon dioxide when reacting with most carbonates.
- All mineral acids are corrosive.
- Noncombustible substances.
- Some are highly reactive to certain substances, causing fire and/or explosions.
- Hydrochloric acid
- Hydrofluoric acid
- Hydroiodic acid
- Phosphoric acid
- Nitric acid
- Sulfuric acid
Organic Cyanides (Nitriles):
These are organic derivatives of Hydrocyanic acid or the cyano-substituted organic compounds.
- Nitriles are highly reactive, the CN group reacts with a large number of reactants to form a wide variety of products, such as amides, amines, carboxylic acids, aldehydes, ketones, esters, thioamides, and other compounds.
- Nitriles are highly toxic compounds, some of them are as toxic as alkali metal cyanides.
- Lower aliphatic nitriles are flammable and form explosive mixtures with air. The explosive range narrows down with an increase in the carbon chain length.
Organic groups attached to the isocyanate group.
- These compounds are highly reactive due to the high unsaturation in the isocyanate functional group.
- Isocyanates in general are highly reactive toward compounds containing active hydrogen atoms.
- Most isocyanates are hazardous to health.
- They are lachrymators and irritants to the skin and mucous membranes.
- Skin contact can cause itching, eczema, and mild tanning.
- Inhalation if isocyanate vapors can produce asthma-like allergic reaction, with symptoms from difficulty in breathing to acute attacks and sudden loss of consciousness.
- Toxicities of isocyanates vary widely, in addition, health hazards differ significantly on the route of exposure but occur primarily via inhalation exposure.
- Most isocyanates have high flash points, therefore the fire hazard is low.
- However, closed containers can rupture due to the pressure built up from carbon dioxide, which is formed from reaction with moisture.
- n-Butyl isocyanate
- Hexamethylene diisocyanate
- Methyl isocyanate
- Phenyl isocyanate
Compounds containing the peroxide group bound to organic groups.
- In general the toxicity is low to moderate.
- Peroxides are a hazardous class of compounds, some of which are extremely dangerous to handle.
- The dangerous ones are highly reactive, powerful oxidizers, highly flammable and often form decomposition products, which are more flammable.
- Many organic peroxides can explode violently due to one or a combination of the follow factors:
- Mechanical shock, such as impact, jarring, or friction
- Chemical contact
- Short chain alkyl and acyl peroxides, hydroperoxides, peroxyesters, and peroxydicarbonates with low carbon numbers are of much greater hazard than the long chain peroxy compounds.
- The active oxygen content of peroxides is measured as the amount of active oxygen (from peroxide functional group) per 100 gm of the substance. The greater the percentage of active oxygen in formulation, the higher is its reactivity. An active oxygen content exceeding 9% is too dangerous for handling and shipping.
- Benzoyl peroxide
- Cumene hydroperoxide
- Diacetyl peroxide
- Diisopropyl peroxydicarbonate
Include certain classes of inorganic compounds that are strong oxidizing agents, evolving oxygen on decomposition.
- These substances are rich in oxygen and decompose violently on heating.
- The explosion hazard arises when these substances come into contact with easily oxidizable compounds such as organics, metals, or metal hydrides.
- When the solid substances are finely divided and combined, the risk of explosion is enhanced.
- The unstable intermediate products, so formed, are sensitive to heat, shock, and percussion.
- The health hazard from the substances arises due to their strong corrosive action on the skin and eyes.
- The toxicity depends on the metal ions in these molecules.
- Inorganic peroxides
There are 2 types: Peroxycarboxylic acids and Peroxysulfonic acids.
- Peroxycarboxylic acids are weaker acids than the corresponding carboxylic acids.
- Lower peroxy acids are volatile liquids, soluble in water.
- Higher acids with greater than 7 carbons are solids and insoluble in water.
- These compounds are highly unstable and can decompose violently on heating.
- May react dangerously with organic matter and readily oxidizable compounds.
- Among organic peroxides, peroxy acids are the most powerful oxidizing compounds.
- The lower acids are also shock sensitive, but less than some organic peroxides.
- Health hazard primarily due to their irritant actions.
- Peroxyacetic acid
- Peroxybenzoic acid
- Peroxyformic acid
Phenols are a class of organic compounds containing hydroxyl groups attached to aromatic rings.
- The hydroxyl group exhibits properties that are different from an alcoholic hydroxyl group.
- Phenols are weakly acidic, forming metal salts on reactions with caustic alkalies.
- In comparison, acid strengths of alcohols are negligibly small or several orders of magnitude lower than those of phenols.
- In comparison with many other classes of organic compounds, phenols show relatively greater toxicity.
These are esters of Phthalic acid.
- They are noncombustible liquids.
- Some are EPA-listed priority pollutants.
- The acute toxicity is very low.
- High doses may produce somnolence, weight loss, dyspnea, and cyanosis.
- The pure liquids are mild irritants to the skin.
- These are relatively harmless and are among the least toxic organic industrial products.
- Dibutyl phthalate
- Diethylhexyl Phthalate (DEHP)
Reference: Patnaik, Pradyot, A Comprehensive Guide to the Hazardous Properties of Chemical Substances, Van Nostrand Reinhold, 1992.
Appendix J - Peroxide Forming ChemicalsAppendix J - Peroxide Forming Chemicals
Peroxide Forming Chemicals
|SAFE STORAGE PERIODS FOR PEROXIDE FORMERS|
|Unopened chemicals from manufacturer||18 months or (expiration date)|
|Chemicals in Table A||3 months|
|Chemicals in Tables B and D||12 months|
|Uninhibited chemicals in Table C||24 hours|
|Inhibited chemicals in Table C (Do not store under an inert atmosphere)||12 months|
|A. Chemicals that form explosive levels of peroxides without concentration|
|Butadienea||Isopropyl ether||Sodium amide (sodamide)|
|Divinylacetylene||Potassium amide||Vinylidene chloride|
|B. Chemicals that form explosive levels of peroxides on concentration|
|Acetaldehyde||Diethylene glycol dimethyl ether (diglyme)||2-Pentanol|
|2-Butanol||Ethylene glycol dimethyl ether (glyme)||1-Phenylethanol|
|Dicyclopentadiene||Methyl isobutyl ketone||Other secondary alcohols|
|C. Chemicals that may autopolymerize as a result of peroxide accumulation|
|Acrylic acidb||Methyl methacrylateb||Vinyl chloride|
D. Chemicals that may form peroxides but cannot clearly be placed in sections A-C
|Allyl etherd||Cyclooctened||n-Hexyl ether|
|Allyl ethyl ether||Cyclopropyl methyl ether||o,p-Iodophenetole|
|Allyl phenyl ether||Diallyl etherd||Isoamyl benzyl etherd|
|p-(n-Amyloxy)benzoyl chloride||p-Di-n-butoxybenzene||Isoamyl etherd|
|n-Amyl ether||1,2-Dibenzyloxyethaned||Isobutyl vinyl ether|
|Benzyl n-butyl etherd||p-Dibenzyloxybenzened||Isophoroned|
|Benzyl etherd||1,2-Dichloroethyl ethyl Ether||B-Isopropoxypropionitriled|
|Benzyl ethyl etherd||2,4-Dichlorophenetole||Isopropyl 2,4,5-trichloro- phenoxy- acetate|
|Benzyl methyl ether||Diethoxymethaned||Limonene|
|Benzyl 1-napthyl etherd||2,2-Diethoxypropane||1,5-p-Methadiene|
|1,2-Bis(2-chloroethoxy) Ethane||Diethyl ethoxymethylene- Malonate||Methyl p-(n-amyloxy)- benzoate|
|Bis(2 ethoxyethyl)ether||Diethyl fumarated||4-Methyl-2-pentanone|
|Bis(2-(methoxyethoxy)- ethyl) ether||Diethyl acetald||n-Methylphenetole|
|Bis(2-methoxyethyl)- Carbonate||Dimethoxymethaned||3-Methoxyethyl acetate|
|Bis(2-methoxyethyl) ether||1,1-Dimethoxyethaned||2-Methoxyethyl vinyl ether|
|Bis(2-methoxyethyl) Phthalate||Dimethylketenef||Methonxy-1,3,5,7-cyclo- octa-tetraene|
|Bis(4-chlorobutyl) ether||Di(1-propynyl)etherf||Oxybis(2-ethyl acetate)|
|Bis(chloromethyl) ethere||Di(2-propynyl)ether||Oxybis(2-ethyl benzoate)|
|2-Bromomethyl ethyl ether||Di-n-propoxymethaned||B,B-oxydipropionitrile|
|o-Bromophenetole||1,2-Epoxy-3-phenoxy- propane||Phenoxyacetyl chloride|
|3-Bromopropyl phenyl ether||2-Ethoxyethyl acetate||Phenyl o-propyl ether|
|Buten-3-yne||1-(2-Ethoxyethoxy)ethyl acetate||n-Propyl ether|
|tert-Butyl ethyl ether||1-Ethoxynaphthalene||n-Propyl isopropyl ether|
|tert-Butyl methyl ether||o,p-Ethoxyphenyl isocyanate||Sodium 8,11,14-eicosa- tetraenoate|
|n-Butyl phenyl ether||1-Ethoxy-2-propyne||Sodium ethoxyacetylidef|
|n-Butyl vinyl ether||3-Ethoxyopropionitrile||Tetrahydropyran|
|Chloroacetaldehyde diethylacetald||2-Ethylacrylaldehyde oxime||Triethylene glycol diacetate|
|2-Chlorobutadiene||2-Ethylbutanol||Triethylene glycol diprop- ionate|
|1-(2-Chloroethoxy)-2- phen-oxyethane||Ethyl B-ethoxypropionate||1,3,3-Trimethoxypropened|
|Chloromethyl methyl ethere||Ethyl vinyl ether||4-Vinyl cyclohexene|
|NOTES: a When stored as a liquid monomer. b Although these chemicals form peroxides, no explosions involving these monomers have been reported. c When stored in liquid form, these chemicals form explosive levels of peroxides without concentration. They may also be stored as a gas in gas cylinders. When stored as a gas, these chemicals may autopolymerize as a result of peroxide accumulation. d These chemicals easily form peroxides and should probably be considered under Part B. e OSHA - regulated carcinogen. f Extremely reactive and unstable compound.|
References: Prudent Practices in the Laboratory, National Research Council, 1995.“Review of Safety Guidelines for Peroxidizable Organic Chemicals,” Chemical Healthand Safety, September/October 1996.
Appendix K - Incompatible ChemicalsAppendix K - Incompatible Chemicals
Substances in the left-hand column should be stored and handled so they cannot contact corresponding substances in the right-hand column. The following list contains some of the chemicals commonly found in laboratories, but it should not be considered exhaustive. Information for the specific chemical you are using can usually be found in the “REACTIVITY” or “INCOMPATIBILITIES” section of the Safety Data Sheet. EHS has a copy of Rapid Guide to Chemical Incompatibilities, by Pohanish and Greene, which lists the incompatibilities of hundreds of chemicals. You may come to our office at 395 Pine Tree Road, Suite 210 and use this valuable reference at any time.
|Chemical Class or Chemical Name||Incompatible Chemicals|
|Alkaline and alkaline earth metals, such as Sodium, Potassium, Cesium, Lithium, Magnesium, Calcium||Carbon dioxide, Carbon tetrachloride and other chlorinated hydrocarbons, any free acid or halogen. Do not use water, foam or dry chemical on fires involving these metals.|
|Acetic acid||Chromic acid, Nitric acid, hydroxyl compounds, Ethylene glycol, Perchloric acid, peroxides, permanganates.|
|Acetic anhydride||Chromic acid, Nitric acid, hydroxyl-containing compounds, Ethylene glycol, Perchloric acid, peroxides and permanganates.|
|Acetone||Concentrated Nitric and Sulfuric acid mixtures.|
|Acetylene||Copper, Silver, Mercury and halogens, Fluorine, Chlorine, Bromine.|
|Alkali & alkaline earth metals (such as powdered Aluminum or Magnesium, Calcium, Lithium, Sodium, Potassium)||Water, Carbon tetrachloride or other chlorinated hydrocarbons, Carbon dioxide, and halogens.|
|Aluminum alkyls||Halogenated hydrocarbons, water.|
|Ammonia (anhydrous)||Silver, Mercury, Chlorine, Calcium hypochlorite, Iodine, Bromine, Hydrogen fluoride, Chlorine dioxide, Hydrofluoric acid (anhydrous).|
|Ammonium nitrate||Acids, metal powders, flammable liquids, chlorates, nitrites, Sulfur, finely divided organics or combustibles.|
|Aniline||Nitric acid, Hydrogen peroxide.|
|Arsenical materials||Any reducing agent.|
|Benzoyl peroxide||Chloroform, organic materials.|
|Bromine||Ammonia, Acetylene, Butadiene, Butane and other petroleum gases, Sodium carbide, Turpentine, Benzene and finely divided metals, Methane, Propane, Hydrogen.|
|Calcium carbide||Water (see also Acetylene).|
|Calcium hypochlorite||Methyl carbitol, Phenol, Glycerol, Nitromethane, Iron oxide, Ammonia, activated carbon.|
|Carbon, activated||Calcium hypochlorite, all oxidizing agents.|
|Chlorates||Ammonium salts, acids, metal powders, Sulfur, finely divided organics or combustibles.|
|Chlorine||Ammonia, Acetylene, Butadiene, Butane, Propane, and other petroleum gases, Hydrogen, Sodium carbide, Turpentine, Benzene and finely divided metals, Methane.|
|Chlorine dioxide||Ammonia, Methane, Phosphine and Hydrogen sulfide.|
|Chlorosulfonic acid||Organic materials, water, powdered metals.|
|Chromic acid & Chromium trioxide||Acetic acid, Naphthalene, Camphor, Glycerin, Turpentine, alcohol and other flammable liquids, paper or cellulose.|
|Copper||Acetylene, Hydrogen peroxide, Ethylene oxide.|
|Cumene hydro peroxide||Acids, organic or mineral.|
|Ethylene oxide||Acids, bases, Copper, Magnesium perchlorate.|
|Flammable liquids||Ammonium nitrate, Chromic acid, Hydrogen peroxide, Nitric acid, Sodium peroxide, halogens.|
|Fluorine||Almost all oxidizable substances.|
|Hydrocarbons (such as Bromine, Butane)||Fluorine, Chlorine, Chromic acid, Sodium peroxide.|
|Hydrocyanic acid||Nitric acid, alkalis.|
|Hydrofluoric acid (anhydrous)||Ammonia (aqueous or anhydrous).|
|Hydrogen peroxide||Copper, Chromium, Iron, most metals or their salts, any flammable liquid, combustible materials, Aniline, Nitromethane, alcohols, Acetone, organic materials, Aniline.|
|Hydrides||Water, air, Carbon dioxide, chlorinated hydrocarbons.|
|Hydrofluoric acid, anhydrous (Hydrogen fluoride)||Ammonia (anhydrous or aqueous), organic peroxides.|
|Hydrogen sulfide||Fuming Nitric acid, oxidizing gases.|
|Hydrocarbons (Benzene, Butane, Propane, Gasoline, Turpentine, etc.)||Fluorine, Chlorine, Bromine, Chromic acid, Sodium peroxide, fuming Nitric acid.|
|Hydroxylamine||Barium oxide, Lead dioxide, Phosphorus pentachloride and trichloride, Zinc, Potassium dichromate.|
|Hypoch lorites||Acids, activated Carbon.|
|Iodine||Acetylene, Ammonia (anhydrous or aqueous), Hydrogen.|
|Maleic anhydride||Sodium hydroxide, Pyridine and other tertiary amines.|
|Mercury||Acetylene, Fulminic acid, Ammonia, Oxalic acid.|
|Nitrates||Acids, metal powders, flammable liquids, chlorates, sulfur, finely divided organics or combustibles, Sulfuric acid.|
|Nitric acid (concentrated)||Acetic acid, Aniline, Chromic acid, Hydrocyanic acid, Hydrogen sulfide, flammable liquids, flammable gases, nitratable substances, organic peroxides, chlorates, Copper, brass, any heavy metals.|
|Nitroparaffins||Inorganic bases, amines.|
|Oxygen||Oil, grease, Hydrogen, flammable liquids, solids, or gases.|
|Oxalic acid||Silver, mercury, organic peroxides.|
|Perchlo ric acid||Acetic anhydride, Bismuth and its alloys, alcohol, paper, wood, grease, oil, organic amines or antioxidants.|
|Peroxides, organic||Acids (organic or mineral); avoid friction, store cold.|
|Phosphorus (white)||Air, Oxygen, alkalis, reducing agents.|
|Phosphorus pentoxide||Propargyl alcohol.|
|Potassium||Carbon tetrachloride, Carbon dioxide, water.|
|Potassium chlorate||Acids, Sulfuric acid (see also chlorates).|
|Potassium perchlorate||Sulfuric & other acids (see also Perchloric acid, & chlorates).|
|Potassium permanganate||Glycerin, Ethylene glycol, Benzaldehyde, any free acid, Sulfuric acid.|
|Silver||Acetylene, Oxalic acid, Tartaric acid, Fulminic acid, ammonium compounds.|
|Sodium||Carbon tetrachloride, Carbon dioxide, water. See alkaline metals (above).|
|Sodium amide||Air, water.|
|Sodium nitrate||Ammonium nitrate and other ammonium salts.|
|Sodium oxide||Water, any free acid.|
|Sodium peroxide||Any oxidizable substance, such as Ethanol, Methanol, glacial Acetic acid, Acetic anhydride, Benzaldehyde, Carbon disulfide, Glycerine, Ethylene glycol, Ethyl acetate, Methyl acetate and Furfural.|
|Sulfuric acid||Chlorates, perchlorates, permanganates, organic peroxides. Potassium chlorate, Potassium perchlorate, Potassium permanganate (similar compounds of light metals, such as Sodium, Lithium).|
|UDMH (1,1-Dimethylhydrazine)||Oxidizing agents such as Hydrogen peroxide and fuming Nitric acid.|
|Zirconium||Prohibit water, Carbon tetrachloride, foam and dry chemical on zirconium fires.|
Appendix L - EHS Segregation SchemeAppendix L - EHS Segregation Scheme Key:
Hazard Class # – All Hazard Classes must be segregated from other Hazard Classes
- Class - must segregate from other Classes within Hazard Class
- Group - recommend to segregate from other groups within class
- Class - must segregate from other Classes within Hazard Class
Hazard Class 1 - Explosives (potentially explosive) Hazard Class 2 - Compressed Gases/Lecture Bottles
Class 2.1 - Flammable gases
- Class 2.2 - Non-Flammable gases
- Class 2.3 - Poisonous gases
- Oxidizing gases (separate from everything)
- Corrosive - acids
- Corrosive - bases
Hazard Class 3 - Flammable liquids
Combustible liquids (that do not have another hazard)
Hazard Class 4 - Flammable solids
Class 4.2 - Spontaneously combustible
- Class 4.3 - Dangerous When Wet
- Class 4.1 - Flammable solids
Hazard Class 5 - Oxidizers
Class 5.1 - Oxidizers
- Class 5.2 - Organic peroxides
Hazard Class 6 - Poisons
Class 6.1 - Poisons
- Reproductive hazards (Teratogens, Mutagens)
- Organic acids, solid
- Nonhazardous chemicals
- Poisonous Inhalation Hazards (PIH)
- Controlled substances
- Class 6.2 - Biohazards - Infectious agents
- CDC Select agents
Hazard Class 7 - Radioactives
Hazard Class 8 - Corrosives
- Oxidizing acids (Nitric acid and Perchloric acid)
- Hydrofluoric acid
- Organic acids, liquid (can store in flammable cabinet)
Appendix M - Sample Prior Approval FormAppendix M - Sample Prior Approval Form
Prior Approval for Highly Hazardous Operations
|PI or Supervisor:__________________________||Location:__________|
|Name of chemical(s) or operation:__________________________________|
Each person on this list should have permission from the lab supervisor or Principal Investigator to use the chemicals or conduct the operation above in this lab and have completed the following:
- Are aware of the hazards the chemical(s) or operation(s) pose?
- Has read the Standard Operating Procedures for this process?
- Knows the first aid procedure in case of an exposure?
- Knows what to do in the event of a spill or other emergency?
- Has received any specific training needed above the standard Lab Safety and Chemical Waste Disposal training?
Appendix N - Sample Prior Approval FormAppendix N - Sample Prior Approval Form
Link to: Laser Registration Form
Appendix O - EHS Reference LibraryAppendix O - EHS Reference Library
Chemical Safety References
There are two references that we recommend for use in determining best practices for managing laboratory chemical hazards.
For teaching laboratories, Safety in Academic Chemistry Laboratories, Volume 1 is written for student use and Volume 2 is written for managers of teaching labs.
For research laboratories, Prudent Practices in the Laboratory can be downloaded from the National Academic Press web site.
For help in interpreting these references, please contact us at askEHS@cornell.edu
Appendix P - Phenol First Aid Guide and PPEAppendix P - Phenol First Aid Guide and PPE
Focus on Phenol First Aid and Personal Protective Equipment
Phenol is a common chemical used for activities such as tissue preservation and DNA/RNA extraction. Phenol can be a component in a commercial reagent (e.g. QIAzol, TRIzol) or prepared as part of a mixture in the laboratory (e.g. chloroform: phenol). Because phenol solutions are an integral part of routine life science applications, their hazards may be taken for granted. Make no mistake about it, however. Phenol can be very dangerous and the hazards are not just those of a typical corrosive.
The hazards of phenol are 2-fold. It is both a corrosive (can cause severe burns) and toxic (absorbed phenol acts as a systemic toxin). In one case, death resulted from ingestion of as little as 15 mL. Liquid phenol can penetrate the skin with efficiency approximately equal to that of inhalation. Deaths have been reported for exposures of 25% or more of body surface area. Phenol has an anesthetic effect and can cause severe burns that may not be immediately painful or visible. The threshold concentration of human skin damage from phenol is 1.5%. It can cause permanent eye injury, blindness and scarring.
Recommendations are that laboratories working with phenol use polyethylene glycol 300 or 400 (PEG-300 or PEG-400), rather than water, for immediate first aid treatment of dermal exposures. In addition, all laboratory groups using phenol should take the need for appropriate personal protective equipment (PPE) seriously and require its use as part of the laboratory operations when using phenol and documented in the Standard Operating Procedures (SOPs). This document can be added as a supplement to existing phenol SOPs.
SYMPTOMS OF PHENOL EXPOSURE
The most common route of occupational exposure for phenol is skin contact and absorption. Phenol does not readily form a vapor at room temperature and is unlikely to pose an inhalation hazard unless it is heated or misted. Additionally, it has a distinct, sweet, acrid, odor that is detected by most people at levels well below the OSHA airborne permissible exposure limit (PEL).
Phenol burns and intoxications can be life-threatening. Symptoms include:
- Eye Contact: Severe irritation, permanent damage, blindness.
- Inhalation: Respiratory irritation, sore throat, headache, and shortness of breath.
- Ingestion: Phenol is very toxic; death can occur rapidly following ingestion. Symptoms include irritation, swelling, burns and damage to the mouth, throat and stomach, internal bleeding, vomiting, diarrhea, decreased blood pressure, shock, collapse, coma and death.
- Skin Contact: Initial exposure can cause numbness or slight tingling, so contact may not be immediately apparent. However, even minor contact can result in burns, blisters, permanent skin damage. Absorption of phenol through skin can result in phenol toxicity with symptoms including muscle weakness, tremors, loss of coordination, shock, sudden collapse, coma, convulsions, organ damage and death. When phenol contacts the skin, a white covering of precipitated protein forms. It soon turns red and eventually sloughs off, leaving the surface stained slightly brown. If phenol is left on the skin, it will penetrate rapidly and lead to cell death and gangrene.
Exposure Control and Personal Protective Equipment
- Emergency Showers and Eyewashes: Any laboratory using phenol (or any corrosive/caustic chemical) must have an emergency eyewash station accessible within 10 seconds and located in the same room the hazard is being used. Emergency showers must be accessible within 10 seconds and can be located within the room or in the hallway.
- Administrative Controls: Never work alone when using phenol. Procedures requiring the use of phenol should have written safety SOPs associated with them, including the location of emergency equipment.
- Engineering Controls: Phenol must be used in a fume hood when working with stock solutions and making formulations and dilutions. Even when working with small amounts of dilute phenol, the best practice is to work in a fume hood because of the splash protection the sash provides and the ability of the hood to contain emissions especially in the event of a spill.
- Eye/Face Protection: Eye protection is required when working with phenol. Chemical splash goggles are best practice and a face shield (in addition to goggles) may also be necessary, depending on concentration and amount. Safety glasses can be worn if working with small quantities of phenol in a fume hood with the sash properly positioned to provide splash, spray and mist protection. Consider that small facial burns caused by splatter may not be life threatening but can result in permanent scarring/disfiguration
- Skin Protection: Lab coat, long sleeves, impervious full foot/closed toe shoes and long pants at a minimum. If body splash potential exists, wear a butyl rubber or neoprene apron.
- Hand Protection: Hand protection needs to be selected based on projected use (concentration and exposure). For working with phenol at concentrations >70%, butyl rubber, Viton, Barrier and Silver Shield gloves provide good resistance. Neoprene and polyvinyl alcohol are suitable for short term work (resistance to breakthrough within 1-4 hours) but should be thicker than 0.3 mm (11.8 mil).
- Thin disposable gloves are generally for splash protection only and should immediately be removed if phenol gets on them. Ansell recommends “NeoTouch®” (neoprene) or DermaShield (proprietary poly-chloroprene blend) for splash protection when working with 10% phenol solutions. A good practice is to use a heavy weight disposable (0.2 mm; 8 mil) and double glove. In general, nitrile is not recommended as a material of choice when working with phenol.
- Chloroform and Phenol Mixtures. Phenol is often used in combination with chloroform in nucleic acid purification procedures. Unfortunately, chloroform rapidly degrades both neoprene and nitrile. Ansell has recently developed a relative thin glove (ChemTek® 380214T) consisting of a 4 mil. outer layer of Viton® rubber over a 4 mil. layer of butyl rubber which provides >90 minutes breakthrough resistance to chloroform/phenol solutions. The request to develop this glove was initiated by the University of California, San Francisco as a result of a phenol/chloroform laboratory accident.
- The gloves demonstrated below (Viton Butyl) may be purchased from VWR, and although they are more expensive than nitrile, they may be reused after incidental contact (always inspect them before use and discard if holes are evident or contamination is apparent).
Eye: Rapid and immediate decontamination is critical. Flush with copious amounts of water using the emergency eyewash for at least 15 minutes, lifting eyelids occasionally. Remove contact lenses if easily removable without additional trauma to the eye. Do not interrupt flushing. Get medical attention immediately
- Inhalation: Remove to fresh air. Get medical attention immediately.
- Ingestion: Do not induce vomiting. If victim is conscious and able to swallow, give 4-8 oz (1 c) of milk or water. Get medical attention immediately. Never give anything by mouth to an unconscious person or while in the laboratory.
- Skin Contact: Rapid and immediate skin decontamination is critical to minimize phenol absorption. Anyone assisting the victim should wear protective clothing and gloves.
- Small Exposures. Rapidly remove contaminated clothing (including anything leather like belts or watchbands) and either irrigate or wipe exposed areas immediately and repeatedly with low-molecular-weight polyethylene glycol (PEG 300 or PEG 400). Treatment should be continued until there is no detectable odor of phenol. If PEG is not available, a gycerine solution can be used instead. If neither of these are available, irrigation with a source of high-density drenching water (such as an emergency shower) will reduce phenol uptake, but lesser amounts of water will merely dilute the phenol and expand the area of exposures. If using the shower or other, shower for at least 15 min.
- Large Exposures. First aid treatment is similar to that for small exposures except the amount of surface area to be decontaminated must be considered. If the amount of phenol on the skin is more than can be quickly removed by swabbing or irrigating with PEG, then an emergency shower should be used and 911 should be called immediately. A high-density shower is preferable to reduce phenol uptake. Lesser amounts of water will merely dilute the phenol and expand the area of exposure. If possible, use PEG after the initial decontamination. Otherwise, the victim should stay in the shower until the emergency responders arrive to provide assistance.
For any exposure, double-bag contaminated clothing and personal belongings. Get medical attention. Even if the exposure is small, it is still important to be evaluated by a medical professional to determine if follow-up treatment is necessary.
Phenol First Aid Kit
Laboratories that use phenol are advised to assemble a kit for first aid treatment of dermal exposure. The kit should be located in a visible area where the phenol work is being done (for instance, in the fume hood), or nearby with the location clearly posted. The principal investigator or laboratory manager should train all persons working with phenol on how to respond to a phenol exposure and how to use the kit.
EHS has designed a simple kit that can fit in a plastic zip-lock bag that laboratories can assemble themselves. Included in the kit is a 500 mL bottle of PEG 300 or PEG 400. Pharmaceutical grade (USP or NF) PEG is recommended and available from most chemical suppliers. A 500 mL bottle is meant to treat small areas of exposure, such as might occur when using DNA/RNA extraction kits. For work involving larger amounts of phenol, contact EHS for help designing a kit and a response plan suitable for the amount of phenol being used.
The recommended contents of the kit and instructions for use are listed below (the list of the contents of the kit can be taped to the outside of the large zip lock bag while the instructions for use should be kept in the bag). Date the bag with the expiration date of the PEG and replace the bottle when it expires (use the opportunity to inspect the integrity of the other items in the kit, such as gloves, and replace as necessary).
Phenol Skin (Dermal) Exposure First Aid Kit:
- Pre-packaged gauze pads, 4” x 4” (~10)
- Polyethylene Glycol 300 or 400, 500 mL (~ 1 pint) USP or NF
- Silver Shield gloves (1-2 pairs)
- Instructions for use
- Small plastic bag (for collecting waste gauze pads)
- Large plastic zip lock bag big enough to hold items above
Instructions Phenol Skin (Dermal) Exposure First Aid
Instructions Phenol Skin (Dermal) Exposure First Aid
Emergency Numbers: 911 (Ithaca campus phone and remote facilities)
(607) 255-1111 (Ithaca cell phone)
NOTE: This procedure is NOT for eye exposure. For eye exposure, flush with water in an eyewash station for at least 15 minutes and call 911 immediately.
Remove contaminated clothing, including any leather items such as watch bands or belts.
- Put on safety glasses and silver shield gloves (but don’t put on the gloves if you are treating yourself and your hands are contaminated with phenol).
- Open up a few packages of gauze pads.
- Pour polyethylene glycol liberally on to one of the gauze pads.
- Gently wipe off excessive phenol on exposed area. Discard the gauze pad in the small plastic bag in the kit.
- Take a new gauze pad, add polyethylene glycol, and continue to clean off exposed area. Discard the pad. Repeat with a new gauze pad and application of polyethylene glycol until all visible traces of phenol have been removed from the skin.
- Continue to gently wipe skin (do not scrape or irritate the effected skin area) with polyethylene glycol soaked gauze pads until no odor of phenol remains, changing the gauze pad frequently.
8. Even if you feel it is unnecessary to call 911 and get emergency help, please go for evaluation to determine if follow-up treatment is necessary. Don’t forget to fill out an on-line incident report! (Incident Reporting)
9. Double bag any contaminated clothing. Label both the bag and the small zip-lock bag for collecting the used gauze wipes as “Hazardous Waste – Phenol Contamination”. Contact EHS for proper disposal. (Waste Pickups)
10. Replace the contents of the kit with new, unused items
Monteiro-Riviere, N. A., A. O. Inman, H. Jackson, B. Dunn and S. Dimond. 2001. Efficacy of topical phenol decontamination strategies on severity of acute phenol chemical burns and dermal absorption: in vitro and in vivo studies in pig skin and Industrial Health. Toxicology and Industrial Health. 17:95-104
Ruth A Lawrence Poison and Drug Information Center (serving the Finger Lakes Region): 1-800-222-1222 (or 1-585-275-3232).
Phenol SOPPhenol SOP
Phenol is a common chemical used for activities such as tissue preservation and DNA/RNA extraction. Phenol can be a component in a commercial reagent (e.g. QIAzol, TRIzol) or prepared as part of a mixture in the laboratory (e.g. chloroform: phenol). It is both a corrosive and toxic. Phenol has an anesthetic effect and can cause severe burns that may not be immediately painful or visible. It can cause permanent eye injury and blindness. The NIOSH Recommended Exposure Level for Phenol is 5 ppm.
2. SPECIFIC HAZARD CLASSIFICATIONS (this section is not a substitution for the review of the Safety Data Sheet - SDS)
|P201||Obtain special instructions before use.|
|P260||Do not breathe dust/fume.|
|P270||Do not eat, drink or smoke when using this product.|
|P271||Use only outdoors or in a well-ventilated area.|
|P280||Wear protective gloves/protective clothing/eye protection/face protection.|
|P281||Use personal protective equipment as required.|
|P273||Avoid release to the environment.|
|P301+P310||If Swallowed: Immediately call a Poison Center or doctor/physician.|
|P301+P330+P331||If Swallowed: Rinse mouth. Do Not induce vomiting.|
|P303+P361+P353||If On Skin (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.|
|P305+P351+P338||If In Eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.|
|P308+P313||If exposed or concerned: Get medical advice/attention.|
|P321||Specific treatment (see advice on this label).|
|P322||Specific measures (see advice on this label).|
|P363||Wash contaminated clothing before reuse.|
|P302+P352||If On Skin: Wash with plenty of water.|
|P304+P340||If Inhaled: Remove victim to fresh air and keep it at rest in a position comfortable for breathing.|
- EHS 2555 Laboratory Safety training
- EHS 2716 Chemical Waste Disposal Training
- EHS 2394 Cleaning Up Small Lab Spills
- Lab Specific – including SDS and SOP review with proficiency on procedure (include where this will be documented in the lab this can be a sign-off attached to the SOP)
4. Ordering OF Phenol and First Aid materials
Company, Catalogue number.
5. Storage and Use
- of reagent in the lab.
- of First Aid Kit
- of Spill Kit
Must be used in a fume hood.
6. Personal Protective Equipment (PPE)
See attached EHS Guidance Document: “Phenol First Aid and PPE”
7. Preparation of Solutions and Working Protocols
Research protocol specifics:
Describe how the samples will be transported to other buildings/rooms.
9. Waste Handling and Disposal
Phenol must be disposed of as hazardous waste – corrosive, toxic
Describe lab-specific process for labeling, storage and removal of hazardous waste.
For light contamination of small areas or items flush with plenty of water and wash items with soap or detergent and water.
Describe your clean up procedures to ensure there is no residue or transfer outside of the hood
- Secure and ventilate the area.
- For small spills wear appropriate PPE retrieve the spill kit from ______________.
- Absorb spill with a spill pad, absorbent material, or lab wipes. Bag spill cleanup materials in plastic bag and manage as hazardous waste.
- For larger spills e.g. over 500mL or if uncomfortable cleaning up:
- Ithaca: Use Emergency Phone or 607-255-1111 for EHS Spill Response. Include location of closest emergency equipment (safety showers, eyewashes, spill kit, First Aid guidance, etc.)
- Geneva specific procedures: call 911
ii. Personnel contamination or exposure: See the EHS Guidance Document: “Phenol First Aid Guide and PPE”
iii Complete incident report for any spill, injury, illness or exposure related to work at Cornell within 24 hours: Incident Reporting.