African Swine Fever Biological Agent Reference Sheet (BARS)
|Agent Type||Risk Group||Biosafety Level||Animal Housing Biosafety Level|
Risk Group: RG-1, not associated with disease in healthy adult
Agent Type: Virus
Description: African swine fever virus (ASFV) is a contagious hemorrhagic disease of wild and domestic pigs. The disease does not pose a risk to human health but is characterized by high morbidity and mortality rates in the swine. Clinical presentation of the virus can range from subclinical infection to severe hemorrhagic disease in pigs. ASFV is part of the Asfivirus genus of the family Asfarviridae and is an enveloped DNA virus. More than 20 genotypes have been found with potential multiple serotypes recently described. Direct contact, indirect contact, and vector-borne (ticks) are the three modes of transmission for ASFV. ASFV is endemic in sub-Saharan Africa and several outbreaks have been observed in Asia and Europe. The disease has never been reported in the United States, Canada, Australia, or New Zealand.
Host Range: All varieties of Sus scrofa (domestic and wild), African wild suid species, and ticks (genus Ornthodoros)
Host Shedding: Blood, feces, urine, direct contact, saliva
Route of Exposure to Humans: This disease does not pose a threat to human health
Infectious Dose: Not applicable
Incubation Period: In swine, 4-19 days for naturally acquired cases
This disease does not pose a threat to human health.
|Survival Outside Host||Disinfection||Inactivation|
|Remains viable for long periods in blood, faeces and tissues, especially uncooked or undercooked pork products. Able to multiply in vectors.||
Susceptible to ether and chloroform.
Inactivated by 8/1000 sodium hydroxide (30 minutes).
Hypochlorites – between 0.03% and 0.5% chlorine (30 minutes), 3/1000 formalin (30 minutes), 3% ortho-phenylphenol (30 minutes) and iodine compounds.
Heat inactivated by 56ºC/70 minutes; 60ºC/20 minutes.
pH inactivated <3.9 or >11.5 in serum-free medium.
For more guidance on disinfection see: disinfectant selection.
- High energy-creating activities (centrifugation, sonication, high pressure systems, vortexing, tube cap popping)
- Handling of sharps (needles, scalpels, microtome blades, broken glass, etc.)
- Splash/droplet-creating activities (shaking incubators, liquid culturing, mechanical pipetting)
- Equipment contamination
- Exposed skin/uncovered wounds
Laboratory Acquired Infection (LAI) History: Not applicable
Laboratory Handling Guidelines
Laboratory Biosafety Level (BSL): BSL-3
Attenuated Strain Alternatives: ASFV-G-Δ9GL/ΔUK and ASFV-G-ΔMGF
|Training||Lab Engineering Controls||Personal Protective Equipment|
Waste Management: Regulated Medical Waste (RMW)
Shipping Guidance: Refer to EHS Biological Materials Shipping
Animal Vivarium Guidance
Animal Housing Biosafety Level (ABSL): ABSL-3
Biosecurity Measures: fences, restricted visitor access, good hygiene, disinfection of footwear or the use of dedicated footwear, closed herds, quarantine of new animals, and other measures help prevent ASFV introduction onto farms of domesticated pigs. Contact with domestic pigs should be avoided, allowing a 5-day interval between hunting wild boars and being in contact with domestic pigs.
Exposure and Spill Procedures
Mucous Membranes: Flush eyes, mouth, or nose for 15 minutes at an eyewash station. See: responding to exposures.
Other Exposures: Wash with soap and water for 15 minutes (open wounds, sores, etc.) or a minimum of 20 seconds for areas with intact skin.
Small Spills: Notify others working in the lab. Don appropriate PPE. For spills involving fecal material, cover area of the spill with paper towels, working from the perimeter toward the center, use the paper towels to remove the spill and associated organic material. Discard contaminated paper towels. For spills involving fecal material and all other spills apply (or re-apply) 6% hydrogen peroxide on the spill site, Allow 20 minutes of contact time. After 20 minutes use paper towels to remove the 6% hydrogen peroxide. See: spill cleanup.
Large Spills: Request assistance from the EHS Spill Team by calling CUPD dispatch. Call 911 from a campus phone or 607-255-1111 from a mobile phone.
Incident Reporting: Immediately report the incident to supervisor and complete the EHS online injury/illness report as soon as possible.
- During Business Hours: Call Cornell Health at 607-255-5155 (24-hour phone consultation line)
- After Hours Care: Call the Cornell Health Services 24-hour phone consultation line or local urgent care as listed on the Cornell Health webpage.
- Emergencies: Call 911 from a campus phone or 607-255-1111 from a mobile phone.
- CDC. (2009). Biosafety in Microbiological and Biomedical Laboratories.
- Gallardo, C., Nieto, R., Soler, A., Pelayo, V., Fernández-Pinero, J., Markowska-Daniel, I., … Arias, M. (2015). Assessment of African swine fever diagnostic techniques as a response to the epidemic outbreaks in eastern european union countries: How to improve surveillance and control programs. Journal of Clinical Microbiology, 53(8), 2555–2565.
- OIE. (2019). CLASSICAL SWINE FEVER (hog cholera). Retrieved from http://www.oie.int/wahis/public.php?page=home]
- USDA. (2019). DISEASE RESPONSE STRATEGY AFRICAN SWINE FEVER. Retrieved from www.aphis.usda.gov/fadprep.